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To control the transmission of HVL, early and accurate detection of infected dogs is paramount importance despite it remains a confronting challenge. Herein, we evaluated the performance of direct agglutination test (DAT) against gold standard nested polymerase chain reaction (nested-PCR) for CVL diagnosis in symptomatic and asymptomatic domestic dogs from endemic areas of Iran.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Venous blood samples were collected from dogs without clinical signs (n \u2009=\u2009 30) and with clinical signs (n \u2009=\u2009 35) suggestive of <jats:italic>Leishmania infantum<\/jats:italic> infection. Among 65 samples examined, <jats:italic>Leishmania<\/jats:italic> DNA was detected by nested-PCR in 89.23% (58\/65). Furthermore, 86.15% (56\/65) nested-PCR positive samples were also DAT positive. The results of the DAT sensitivity test were 96.43% and 96.67% in symptomatic and asymptomatic dogs, respectively, while the specificity was 100.00% and 60.00% in symptomatic and asymptomatic dogs, respectively. The results of this study also pointed out substantial concordance between DAT test and nested-PCR method in both symptomatic dogs (\u039a \u2009=\u2009 0.783; P \u2009&lt;\u2009 0.001) and asymptomatic dogs (\u039a \u2009=\u2009 0.618; P \u2009&lt;\u2009 0.001). Thus, DAT represents as a simple and economic tool for initial diagnosis of CVL particularly in endemic areas of the disease.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-021-05654-0","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T11:10:29Z","timestamp":1626174629000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A comparative study of nested-PCR and direct agglutination test (DAT) for the detection of Leishmania infantum infection in symptomatic and asymptomatic domestic dogs"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5873-0239","authenticated-orcid":false,"given":"Yonas","family":"Yimam Ayene","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Homa","family":"Hajjaran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Behnaz","family":"Akhoundi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saeedeh","family":"Shojaee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbas","family":"Rahimi-Foroushani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Javad Abbaszadeh","family":"Afshar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Zarei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,7,13]]},"reference":[{"issue":"11\u201312","key":"5654_CR1","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1016\/j.ijpara.2005.07.001","volume":"35","author":"M Gramiccia","year":"2005","unstructured":"Gramiccia M, Gradoni L. 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Dog owners were informed of the study objectives and voluntary verbal and written consent were obtained.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"270"}}