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Phage \u03d51207.3 of <jats:italic>Streptococcus pyogenes<\/jats:italic>, carrying the <jats:italic>mef<\/jats:italic>(A)\u2013<jats:italic>msr<\/jats:italic>(D) gene pair, is able to transfer the macrolide efflux phenotype to <jats:italic>Streptococcus pneumoniae<\/jats:italic>. Deletion of <jats:italic>mef<\/jats:italic>(A) in pneumococcal \u03d51207.3-carrying strains did not affect erythromycin efflux. In order to identify candidate genes likely involved in complementation of <jats:italic>mef(<\/jats:italic>A) deletion, the Mef(A) amino acid sequence was used as probe for database searching.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In silico analysis identified 3 putative candidates in the <jats:italic>S. pneumoniae<\/jats:italic> R6 genome, namely <jats:italic>spr0971<\/jats:italic>, <jats:italic>spr1023<\/jats:italic> and <jats:italic>spr1932<\/jats:italic>. Isogenic deletion mutants of each candidate gene were constructed and used in erythromycin sensitivity assays to investigate their contribution to <jats:italic>mef<\/jats:italic>(A) complementation. Since no change in erythromycin sensitivity was observed compared to the parental strain, we produced double and triple mutants to assess the potential synergic activity of the selected genes. Also these mutants did not complement the <jats:italic>mef<\/jats:italic>(A) function.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-021-05856-6","type":"journal-article","created":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T18:32:03Z","timestamp":1638297123000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Predicted transmembrane proteins with homology to Mef(A) are not responsible for complementing mef(A) deletion in the mef(A)\u2013msr(D) macrolide efflux system in Streptococcus pneumoniae"],"prefix":"10.1186","volume":"14","author":[{"given":"Valeria","family":"Fox","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Santoro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianni","family":"Pozzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5064-1496","authenticated-orcid":false,"given":"Francesco","family":"Iannelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"issue":"3","key":"5856_CR1","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1128\/AAC.39.3.577","volume":"39","author":"B Weisblum","year":"1995","unstructured":"Weisblum B. 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