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Ten week-old male mice were subjected to r-mTBI once per day for 4\u00a0days followed by closed femoral fracture using a three-point bending technique, 1\u00a0week post impact and fracture healing phenotype evaluated at 20\u00a0weeks of age.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Micro-CT analysis of the fracture callus region at nine weeks post fracture revealed reduced bone volume (30%,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009\n                      <jats:italic>0.05<\/jats:italic>\n                      ) in the r-mTBI fracture group compared to the control-fracture group. The connectivity density of the fracture callus bone was reduced by 40% (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009\n                      <jats:italic>0.01)<\/jats:italic>\n                      in the r-mTBI fracture group. Finite element analysis of the fracture callus region showed reduced failure load (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009\n                      <jats:italic>0.08<\/jats:italic>\n                      ) in the r-mTBI group compared to control group. There was no residual cartilage in the fracture callus region of either the r-mTBI or control fracture group. The reduced fracture callus bone volume and mechanical strength of fracture callus in r-mTBI mice 9\u00a0weeks post fracture are consistent with negative effects of r-mTBI on fracture healing over a long-term resulting in decreased mechanical strength of the fracture callus.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13104-022-05906-7","type":"journal-article","created":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T13:02:37Z","timestamp":1643461357000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Repeated mild traumatic brain injury impairs fracture healing in male mice"],"prefix":"10.1186","volume":"15","author":[{"given":"Chandrasekhar","family":"Kesavan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Rundle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0063-986X","authenticated-orcid":false,"given":"Subburaman","family":"Mohan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,29]]},"reference":[{"key":"5906_CR1","volume":"2012","author":"CS Sahler","year":"2012","unstructured":"Sahler CS, Greenwald BD. 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