{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T10:42:18Z","timestamp":1785753738081,"version":"3.56.0"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T00:00:00Z","timestamp":1646265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Objective<\/jats:title>\n                <jats:p>Cholera is an intestinal infection caused by <jats:italic>Vibrio cholerae<\/jats:italic>, it is usually occurs in developing countries that lack of sanitation. In developing country including Indonesia, awareness importance of sanitation is still low. Unfortunately, research related to the detection of <jats:italic>V. cholerae<\/jats:italic> from fruit and vegetables in Indonesia is still rare. In this study, MPN method was used to determine the prevalence of <jats:italic>V. cholerae<\/jats:italic> followed by single and multiplex PCR to detect virulence genes, including <jats:italic>toxR, ctxA, tcpA<\/jats:italic>, <jats:italic>hlyA<\/jats:italic>, <jats:italic>ace<\/jats:italic>, <jats:italic>ompU<\/jats:italic>, and <jats:italic>zot<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We found 3 fruits and 2 vegetables positive for <jats:italic>toxR<\/jats:italic> gene. Fruit samples which were showed <jats:italic>toxR<\/jats:italic> positive found from East Jakarta while for vegetables, it was recovered from West Jakarta and Central Jakarta. Twenty-three isolates were recovered from <jats:italic>toxR<\/jats:italic> positive samples. The result of antibiotic resistance analysis showed that 4.35% of the isolates resistant to gentamicin, streptomycin (17.39%), trimethoprim (52.17%), ciprofloxacin (30.43%), ampicillin (13.04%), nalidixic acid (82.61%), and polymyxin B (91.30%). None of these isolates were resistant to kanamycin. Combination of MPN and Multiplex PCR method can be used to detect the prevalence and characterize the virulence properties of <jats:italic>V. cholerae<\/jats:italic>.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-022-05955-y","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T01:13:54Z","timestamp":1646270034000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prevalence and molecular characterization of Vibrio cholerae from fruits and salad vegetables sold in Jakarta, Indonesia, using most probable number and PCR"],"prefix":"10.1186","volume":"15","author":[{"given":"Andrea","family":"Budiman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Kurnia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6013-7979","authenticated-orcid":false,"given":"Diana E.","family":"Waturangi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,3]]},"reference":[{"issue":"6","key":"5955_CR1","doi-asserted-by":"publisher","first-page":"e0003832","DOI":"10.1371\/journal.pntd.0003832","volume":"9","author":"M Ali","year":"2015","unstructured":"Ali M, Nelson AR, Lopez AL, Sack DA. 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