{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T12:51:01Z","timestamp":1793019061845,"version":"4.5.0"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Spermatogenesis is the process of producing mature sperm from Spermatogonial stem cells (SSCs) and this process requires a complex cooperation of different types of somatic and germ cells. Undifferentiated spermatogonia initiate the spermatogenesis and Sertoli cells as the only somatic cells inside of the seminiferous tubule play a key role in providing chemical and physical requirements for normal spermatogenesis, here, we investigated the dysfunction of these cells in non-obstructive azoospermia.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Material and method<\/jats:title>\n                <jats:p>In this study, we analyzed the expression of sox9 and UTF1 in the non-obstructive human testis by immunohistochemistry. Moreover, we used the KEGG pathway and bioinformatics analysis to reveal the connection between our object genes and protein.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The immunohistochemistry analysis of the non-obstructive human seminiferous tubule showed low expression of Sox9 and UTF1 that was detected out of the main location of the responsible cells for these expressions. Our bioinformatics analysis clearly and strongly indicated the relation between UTF1 in undifferentiated spermatogonia and Sox9 in Sertoli cells mediated by POU5F1.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Generally, this study showed the negative effect of POU5F1 as a mediator between Sertoli cells as the somatic cells within seminiferous tubules and undifferentiated spermatogonia as the spermatogenesis initiator germ cells in non-obstructive conditions.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-024-06711-0","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T23:01:23Z","timestamp":1710457283000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sox9 downregulation in non-obstructive azoospermia by UTF1 and mediator role of POU5F1"],"prefix":"10.1186","volume":"17","author":[{"given":"Mehdi Mehdinezhad","family":"Roshan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Azizi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadreza Ashtari","family":"Majelan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amirreza Niazi","family":"Tabar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"issue":"3","key":"6711_CR1","first-page":"R95","volume":"157","author":"PA Parekh","year":"2019","unstructured":"Parekh PA, Garcia TX, Hofmann M-C. Regulation of GDNF expression in sertoli cells. Reproduction. 2019;157(3):R95\u2013R107.","journal-title":"Reproduction"},{"issue":"10","key":"6711_CR2","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.3390\/cells8101156","volume":"8","author":"S Lee","year":"2019","unstructured":"Lee S, et al. Differential Regulation of TLE3 in sertoli cells of the testes during postnatal development. Cells. 2019;8(10):1156.","journal-title":"Cells"},{"key":"6711_CR3","unstructured":"Azizi H et al. Investigation of VASA Gene and protein expression in neonate and adult testicular germ cells in mice in vivo and in Vitro. Cell J (Yakhteh), 2020. 22(2)."},{"issue":"1","key":"6711_CR4","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1111\/cen.14122","volume":"92","author":"C Pelusi","year":"2020","unstructured":"Pelusi C, et al. Effect of clomiphene citrate treatment on the sertoli cells of dysmetabolic obese men with low testosterone levels. Clin Endocrinol. 2020;92(1):38\u201345.","journal-title":"Clin Endocrinol"},{"key":"6711_CR5","doi-asserted-by":"crossref","unstructured":"Paduch DA et al. Aberrant gene expression by sertoli cells in infertile men with sertoli cell-only syndrome. PLoS ONE, 2019. 14(5).","DOI":"10.1371\/journal.pone.0216586"},{"key":"6711_CR6","doi-asserted-by":"crossref","unstructured":"Miller SR et al. Cell model for studying Nucleoside transporters, a key component of the blood-testis barrier. FASEB J, 2019. 33(1_supplement): p. 507.12-507.12.","DOI":"10.1096\/fasebj.2019.33.1_supplement.507.12"},{"issue":"4","key":"6711_CR7","doi-asserted-by":"publisher","first-page":"e1008509","DOI":"10.1371\/journal.ppat.1008509","volume":"16","author":"L Hui","year":"2020","unstructured":"Hui L, et al. Matrix metalloproteinase 9 facilitates Zika virus invasion of the testis by modulating the integrity of the blood-testis barrier. PLoS Pathog. 2020;16(4):e1008509.","journal-title":"PLoS Pathog"},{"key":"6711_CR8","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/j.ecoenv.2019.01.007","volume":"171","author":"S Tao","year":"2019","unstructured":"Tao S, et al. Adverse effects of bisphenol A on sertoli cell blood-testis barrier in rare minnow Gobiocypris rarus. Ecotoxicol Environ Saf. 2019;171:475\u201383.","journal-title":"Ecotoxicol Environ Saf"},{"key":"6711_CR9","doi-asserted-by":"crossref","unstructured":"Su L, et al. Testin regulates the blood-testis barrier via disturbing occludin\/ZO\u20101 association and actin organization. Journal of Cellular Physiology; 2020.","DOI":"10.1002\/jcp.29541"},{"issue":"7","key":"6711_CR10","doi-asserted-by":"publisher","first-page":"1393","DOI":"10.1111\/febs.14779","volume":"286","author":"RL Bernardino","year":"2019","unstructured":"Bernardino RL, et al. Carbonic anhydrases are involved in mitochondrial biogenesis and control the production of lactate by human sertoli cells. FEBS J. 2019;286(7):1393\u2013406.","journal-title":"FEBS J"},{"issue":"8","key":"6711_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41419-019-1782-z","volume":"10","author":"F-D Ni","year":"2019","unstructured":"Ni F-D, Hao S-L, Yang W-X. Multiple signaling pathways in sertoli cells: recent findings in spermatogenesis. Cell Death Dis. 2019;10(8):1\u201315.","journal-title":"Cell Death Dis"},{"issue":"1","key":"6711_CR12","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.devcel.2006.05.010","volume":"11","author":"K Nayernia","year":"2006","unstructured":"Nayernia K, et al. In vitro-differentiated embryonic stem cells give rise to male gametes that can generate offspring mice. 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All methods were performed in accordance with the relevant guidelines and regulations.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"77"}}