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By combining electric cell-substrate impedance sensing, with next-generation-sequencing technology, we showed that mRNA gene expression profiles and related pathways can depend on the growth phase of IPEC-J2 cells. Our investigative approach welcomes scientists to reproduce or modify our protocols and endorses putting their gene expression data in the context of the respective growth phase of the cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Three time points are presented: (TP1) 1\u00a0h after medium change (=\u20096\u00a0h after seeding of cells), (TP2) the time point of the first derivative maximum of the cell growth curve, and a third point at the beginning of the plateau phase (TP3). Significantly outstanding at TP1 compared to TP2 was upregulated PLEKHN1, further FOSB and DEGS2 were significantly downregulated at TP2 compared to TP3. Any provided data can be used to improve next-generation experiments with IPEC-J2 cells.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-024-06812-w","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T12:12:57Z","timestamp":1717589577000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Growth behavior and mRNA expression profiling during growth of IPEC-J2 cells"],"prefix":"10.1186","volume":"17","author":[{"given":"A. 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