{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T11:46:05Z","timestamp":1768823165072,"version":"3.49.0"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Objective<\/jats:title>\n                <jats:p><jats:italic>Bartonella<\/jats:italic> are emerging bacterial zoonotic pathogens. Utilization of clotted blood samples for surveillance of these bacteria in wildlife has begun to supersede the use of tissues; however, the efficacy of these samples has not been fully investigated. Our objective was to compare the efficacy of spleen and blood samples for DNA extraction and direct detection of <jats:italic>Bartonella<\/jats:italic> spp. via qPCR. In addition, we present a protocol for improved DNA extraction from clotted, pelleted (i.e., centrifuged) blood samples obtained from wild small mammals.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>DNA concentrations from kit-extracted blood clot samples were low and A260\/A280 absorbance ratios indicated high impurity. Kit-based DNA extraction of spleen samples was efficient and produced ample DNA concentrations of good quality. We developed an in-house extraction method for the blood clots which resulted in apposite DNA quality when compared to spleen samples extracted via MagMAX DNA Ultra 2.0 kit. We detected <jats:italic>Bartonella<\/jats:italic> in 9\/30 (30.0%) kit-extracted spleen DNA samples and 11\/30 (36.7%) in-house-extracted blood clot samples using PCR. Our results suggest that kit-based methods may be less suitable for DNA extraction from blood clots, and that blood clot samples may be superior to tissues for <jats:italic>Bartonella<\/jats:italic> detection.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-024-06841-5","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T14:03:41Z","timestamp":1719929021000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Using clotted, pelleted blood samples for direct molecular detection of Bartonella spp. in small mammal wildlife surveillance studies"],"prefix":"10.1186","volume":"17","author":[{"given":"Simon P.","family":"Jeeves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Champika","family":"Fernando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathon D.","family":"Kotwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"Mubareka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet E.","family":"Hill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire M.","family":"Jardine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,2]]},"reference":[{"key":"6841_CR1","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1007\/978-4-431-55120-1_6","volume-title":"Confronting emerging zoonoses","author":"EL Pultorak","year":"2014","unstructured":"Pultorak EL, Maggi RG, Breitschwerdt EB. Bartonellosis: a One Health perspective. In: Yamada A, Kahn LH, Kaplan B, Monath TP, Woodall J, Conti L, editors. Confronting emerging zoonoses. Tokyo: Springer Japan; 2014. pp. 113\u201349."},{"key":"6841_CR2","doi-asserted-by":"publisher","first-page":"5607","DOI":"10.1099\/ijsem.0.004332","volume":"70","author":"AC Parte","year":"2020","unstructured":"Parte AC, Sard\u00e0 Carbasse J, Meier-Kolthoff JP, Reimer LC, G\u00f6ker M. List of prokaryotic names with standing in nomenclature (LPSN) moves to the DSMZ. Int J Syst Evol Microbiol. 2020;70:5607\u201312.","journal-title":"Int J Syst Evol Microbiol"},{"key":"6841_CR3","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1017\/S0031182017001263","volume":"145","author":"M Kosoy","year":"2018","unstructured":"Kosoy M, McKee C, Albayrak L, Fofanov Y. Genotyping of Bartonella bacteria and their animal hosts: current status and perspectives. Parasitology. 2018;145:543\u201362.","journal-title":"Parasitology"},{"key":"6841_CR4","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/s13071-022-05162-5","volume":"15","author":"M Kr\u00fcgel","year":"2022","unstructured":"Kr\u00fcgel M, Kr\u00f3l N, Kempf VAJ, Pfeffer M, Obiegala A. Emerging rodent-associated Bartonella: a threat for human health? Parasit Vectors. 2022;15:113.","journal-title":"Parasit Vectors"},{"key":"6841_CR5","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1089\/vbz.2016.1956","volume":"17","author":"R Guti\u00e9rrez","year":"2017","unstructured":"Guti\u00e9rrez R, Vayssier-Taussat M, Buffet J-P, Harrus S. Guidelines for the isolation, molecular detection, and characterization of Bartonella species. 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