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Thus, BCG vaccination induces both specific immunity against mycobacteria and non-specific \u201ctrained immunity\u201d against various pathogens. To understand the fundamental mechanisms of \u201ctrained\u201d immunity, studies of transcriptome changes occurring during BCG vaccination in innate immunity cells, as well as in their precursors, are necessary. Furthermore, this data possesses important significance for practical applications associated with the development of recombinant BCG strains aimed to enhance innate immunity against diverse infectious agents.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Data description<\/jats:title>\n                <jats:p>We performed RNA sequencing of innate immune cells derived from murine bone marrow and spleen three days after subcutaneous BCG vaccination. Using fluorescence-activated cell sorting we obtained three cell populations for each mouse from both control and BCG vaccinated groups: bone marrow monocytes and neutrophils and splenic NK-cells. Then double-indexed cDNA libraries for Illumina sequencing from the collected samples were prepared, the resulting cDNA library mix was subjected to NovaSeq 6000 sequencing. This paper describes the collection of 24 RNA sequencing samples comprising 4 sets of immune cell populations obtained from subcutaneously BCG-vaccinated and control mice<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-024-06901-w","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T20:24:37Z","timestamp":1725913477000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Early transcriptomic response of innate immune cells to subcutaneous BCG vaccination of mice"],"prefix":"10.1186","volume":"17","author":[{"given":"Liya","family":"Kondratyeva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexey","family":"Kuzmich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Linge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Pleshkan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Rakitina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Kondratieva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene","family":"Snezhkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Sass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Alekseenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,9,9]]},"reference":[{"issue":"1","key":"6901_CR1","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1093\/trstmh\/tru168","volume":"109","author":"J Kleinnijenhuis","year":"2015","unstructured":"Kleinnijenhuis J, van Crevel R, Netea MG. Trained immunity: consequences for the heterologous effects of BCG vaccination. Trans R Soc Trop Med Hyg. 2015;109(1):29\u201335.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"6901_CR2","doi-asserted-by":"publisher","first-page":"2631","DOI":"10.3389\/fimmu.2019.02631","volume":"10","author":"B Agrawal","year":"2019","unstructured":"Agrawal B. Heterologous immunity: role in Natural and Vaccine-Induced Resistance to infections. Front Immunol. 2019;10:2631.","journal-title":"Front Immunol"},{"key":"6901_CR3","doi-asserted-by":"crossref","unstructured":"Trunk G, Davidovic M, Bohlius J. Non-specific effects of Bacillus Calmette-Guerin: a systematic review and Meta-analysis of Randomized controlled trials. Vaccines (Basel) 2023, 11(1).","DOI":"10.3390\/vaccines11010121"},{"issue":"1","key":"6901_CR4","doi-asserted-by":"publisher","first-page":"152052","DOI":"10.1016\/j.imbio.2020.152052","volume":"226","author":"AJ Moulson","year":"2021","unstructured":"Moulson AJ, Av-Gay Y. BCG immunomodulation: from the \u2018hygiene hypothesis\u2019 to COVID-19. Immunobiology. 2021;226(1):152052.","journal-title":"Immunobiology"},{"issue":"7","key":"6901_CR5","doi-asserted-by":"publisher","first-page":"108387","DOI":"10.1016\/j.celrep.2020.108387","volume":"33","author":"S Moorlag","year":"2020","unstructured":"Moorlag S, Rodriguez-Rosales YA, Gillard J, Fanucchi S, Theunissen K, Novakovic B, de Bont CM, Negishi Y, Fok ET, Kalafati L, et al. BCG Vaccination induces long-term functional reprogramming of human neutrophils. Cell Rep. 2020;33(7):108387.","journal-title":"Cell Rep"},{"issue":"7274","key":"6901_CR6","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1136\/bmj.321.7274.1435","volume":"321","author":"I Kristensen","year":"2000","unstructured":"Kristensen I, Aaby P, Jensen H. Routine vaccinations and child survival: follow up study in Guinea-Bissau, West Africa. 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The studies using mice were reviewed and approved by the Animal Care and Use Committee of the Central Institute for Tuberculosis, Moscow, Russia (protocols 1 and 6 from 03 March 2022). All animal manipulations were performed according to the recommendations of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes, Council of Europe (ETS 123).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"253"}}