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Rural populations in Egypt lack access to clean water. They obtain their water supplies from different drains and canals, which are frequently exposed to contamination by human activities and untreated agricultural waste, introducing <jats:italic>Cryptosporidium<\/jats:italic> infection to unprotected waterways. A total of (72) canal water samples served for the molecular detection of <jats:italic>Cryptosporidium<\/jats:italic> species by PCR amplification and sequencing.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Only one sample 1.4% (1\/72) belonging to the village of Al-Aziziyyah was PCR-positive for <jats:italic>Cryptosporidium<\/jats:italic> contamination. Based on the (<jats:italic>COWP<\/jats:italic>) gene sequencing, this species was identified as <jats:italic>Cryptosporidium parvum (C. parvum).<\/jats:italic> The current work\u2019s findings marked the first report on the molecular characterization of <jats:italic>Cryptosporidium<\/jats:italic> species in the canal water of Sharqia Province. Our results suggested that the source of <jats:italic>C. parvum<\/jats:italic> contamination could be of human and\/or animal origin. Therefore, further studies are warranted to track the source of human infection and mitigate contamination for improved water quality.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s13104-025-07360-7","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T15:20:00Z","timestamp":1752592800000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt"],"prefix":"10.1186","volume":"18","author":[{"given":"Marwa","family":"Omar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samia E.","family":"Etewa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahani I.","family":"Farag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samar Abd","family":"EL-Nabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heba","family":"Abdelal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"7360_CR1","doi-asserted-by":"publisher","first-page":"1789","DOI":"10.1007\/s00436-016-4917-5","volume":"115","author":"J Trivi\u00f1o-Valencia","year":"2016","unstructured":"Trivi\u00f1o-Valencia J, Lora F, Zuluaga JD, Gomez-Marin JE. 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