{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:02:47Z","timestamp":1773100967961,"version":"3.50.1"},"reference-count":8,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T00:00:00Z","timestamp":1770336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":31,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"name":"National Institute of Allergies and Infectious Diseases, National Institutes of Health","award":["AI148787"],"award-info":[{"award-number":["AI148787"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"DOI":"10.1186\/s13104-026-07652-6","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T09:09:18Z","timestamp":1770368958000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["SSA-like and MMEJ-like recombination events in a transgenic construct with in-cis programmed self-eliminating components in different E. coli strains"],"prefix":"10.1186","volume":"19","author":[{"given":"Christian E.","family":"Ogaugwu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zach N.","family":"Adelman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,6]]},"reference":[{"issue":"1","key":"7652_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-06348-6","volume":"7","author":"K Chae","year":"2024","unstructured":"Chae K, Contreras B, Romanowski JS, Dawson C, Myles KM, Adelman ZN. Transgene removal using an in cis programmed homing endonuclease via single-strand annealing in the mosquito Aedes aegypti. Commun Biol. 2024;7(1):660. https:\/\/doi.org\/10.1038\/s42003-024-06348-6.","journal-title":"Commun Biol"},{"issue":"11","key":"7652_CR2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011450","volume":"20","author":"PR Chennuri","year":"2024","unstructured":"Chennuri PR, Zapletal J, Monfardini RD, Ndeffo-Mbah ML, Adelman ZN, Myles KM. Repeat mediated excision of gene drive elements for restoring wild-type populations. PLoS Genet. 2024;20(11):e1011450. https:\/\/doi.org\/10.1371\/journal.pgen.1011450.","journal-title":"PLoS Genet"},{"issue":"2","key":"7652_CR3","doi-asserted-by":"publisher","DOI":"10.1093\/pnasnexus\/pgac037","volume":"1","author":"K Chae","year":"2022","unstructured":"Chae K, Dawson C, Valentin C, Contreras B, Zapletal J, Myles KM, et al. Engineering a self-eliminating transgene in the yellow fever mosquito, Aedes aegypti. PNAS Nexus. 2022;1(2):pgac037. https:\/\/doi.org\/10.1093\/pnasnexus\/pgac037.","journal-title":"PNAS Nexus"},{"issue":"1","key":"7652_CR4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008103","volume":"16","author":"A Buchman","year":"2020","unstructured":"Buchman A, Gamez S, Li M, Anotsheckhkin I, Li H-H, Wang H-W, et al. Broad dengue neutralization in mosquitoes expressing an engineered antibody. PLoS Pathog. 2020;16(1):e1008103. https:\/\/doi.org\/10.1371\/journal.ppat.1008103.","journal-title":"PLoS Pathog"},{"issue":"37","key":"7652_CR5","doi-asserted-by":"publisher","first-page":"22805","DOI":"10.1073\/pnas.2010214117","volume":"117","author":"R Carballar-Lejarazu","year":"2020","unstructured":"Carballar-Lejarazu R, Ogaugwu C, Tushar T, Kelsey A, Pham TB, Murphy J, et al. Next-generation gene drive for population modification of the malaria vector mosquito,\u00a0Anopheles gambiae. Proc Natl Acad Sci U S A. 2020;117(37):22805\u201314. https:\/\/doi.org\/10.1073\/pnas.2010214117.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"29","key":"7652_CR6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2221118120","volume":"120","author":"R Carballar-Lejarazu","year":"2023","unstructured":"Carballar-Lejarazu R, Dong Y, Pham TB, Tushar T, Corder RM, Mondal A, et al. Dual effector population modification gene-drive strains of the African malaria mosquitoes,\u00a0Anopheles gambiae\u00a0and\u00a0Anopheles coluzzii. PNAS. 2023;120(29):e2221118120. https:\/\/doi.org\/10.1073\/pnas.2221118120.","journal-title":"PNAS"},{"issue":"5","key":"7652_CR7","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1038\/nmeth.1318","volume":"6","author":"DG Gibson","year":"2009","unstructured":"Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009;6(5):343\u20135. https:\/\/doi.org\/10.1038\/nmeth.1318.","journal-title":"Nat Methods"},{"issue":"18","key":"7652_CR8","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21186461","volume":"21","author":"H Yang","year":"2020","unstructured":"Yang H, Ren S, Yu S, Pan H, Li T, Ge S, et al. Methods favouring homology-directed repair choice in response to CRISPR\/Cas9 induced-double strand breaks. Int J Mol Sci. 2020;21(18):6461. https:\/\/doi.org\/10.3390\/ijms21186461.","journal-title":"Int J Mol Sci"}],"container-title":["BMC Research Notes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13104-026-07652-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13104-026-07652-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13104-026-07652-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T13:16:10Z","timestamp":1773062170000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s13104-026-07652-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,6]]},"references-count":8,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["7652"],"URL":"https:\/\/doi.org\/10.1186\/s13104-026-07652-6","relation":{},"ISSN":["1756-0500"],"issn-type":[{"value":"1756-0500","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,6]]},"assertion":[{"value":"5 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"110"}}