{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:59:28Z","timestamp":1780091968776,"version":"3.54.0"},"reference-count":7,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T00:00:00Z","timestamp":1569283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T00:00:00Z","timestamp":1569283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18fk0108019"],"award-info":[{"award-number":["JP18fk0108019"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Med Case Reports"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n              <jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Torque teno virus-induced aseptic meningitis has not been documented, although torque teno virus infections still remain under consideration for etiological agents. This study identified a torque teno virus sequence using next generation sequencing and immunoglobulin M response to the torque teno virus antigen, therefore, that would be a comprehensive diagnosis for torque teno virus infection.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Case presentation<\/jats:title>\n                <jats:p>A 2-month-old Japanese boy was brought to our hospital because he was irritable, drowsy, and lethargic. He was admitted based on his test results which indicated the possibility of septic meningitis. He was started on treatment with high-dose antibiotics and steroids. On the third day of hospitalization, he became afebrile with improvement in his general status and was discharged on the sixth day. He had no developmental problems for up to 1 year after discharge. Metagenomic ribonucleic acid-Seq pathogen detection using next generation sequencing of a sample of his cerebrospinal fluid, which was collected at admission, revealed three short reads homologous to those in torque teno virus out of a total of 1,708,516 reads. This finding indicated that our patient was positive compared to the torque teno virus-negative cerebrospinal fluid samples (controls) from 13 other patients. The torque teno virus has been shown to have a whole genome sequence of 2810\u2009nt by polymerase chain reaction. We prepared a recombinant GP2 antigen from torque teno virus and used it to study our patient\u2019s anti-torque teno virus immune response. An anti-GP2 serum immunoglobulin M response was detected, providing further supportive evidence of torque teno virus infection.<\/jats:p>\n              <\/jats:sec>\n              <jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>This case speculates that torque teno virus-induced aseptic meningitis has a good course. New technologies like next generation sequencing can help in the identification of such cases, and an accumulation of future cases is expected.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13256-019-2233-2","type":"journal-article","created":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T00:02:31Z","timestamp":1569283351000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Aseptic meningitis caused by torque teno virus in an infant: a case report"],"prefix":"10.1186","volume":"13","author":[{"given":"Yoji","family":"Ikuta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiro","family":"Oba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emina","family":"Nai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuo","family":"Katori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Hashino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuba","family":"Inamine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoko","family":"Matsunaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaro","family":"Yamaoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Sekizuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihide","family":"Ryo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0487-4405","authenticated-orcid":false,"given":"Makoto","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,24]]},"reference":[{"issue":"1","key":"2233_CR1","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1006\/bbrc.1997.7765","volume":"241","author":"T Nishizawa","year":"1997","unstructured":"Nishizawa T, Okamoto H, Konishi K, et al. A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochem Biophys Res Commun. 1997;241(1):92\u20137. \n                    https:\/\/doi.org\/10.1006\/bbrc.1997.7765\n                    \n                  . PubMed PMID: 9405239","journal-title":"Biochem Biophys Res Commun"},{"issue":"4","key":"2233_CR2","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1007\/s00705-015-2363-9","volume":"160","author":"S Spandole","year":"2015","unstructured":"Spandole S, Cimponeriu D, Berca LM, et al. Human anelloviruses: an update of molecular, epidemiological and clinical aspects. Arch Virol. 2015;160(4):893\u2013908. \n                    https:\/\/doi.org\/10.1007\/s00705-015-2363-9\n                    \n                  . PubMed PMID: 25680568","journal-title":"Arch Virol"},{"key":"2233_CR3","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.jcv.2016.03.010","volume":"78","author":"M Sato","year":"2016","unstructured":"Sato M, Kuroda M, Kasai M, et al. Acute encephalopathy in an immunocompromised boy with astrovirus-MLB1 infection detected by next generation sequencing. J Clin Virol. 2016;78:66\u201370. \n                    https:\/\/doi.org\/10.1016\/j.jcv.2016.03.010\n                    \n                  . PubMed PMID: 26991054","journal-title":"J Clin Virol"},{"issue":"12","key":"2233_CR4","doi-asserted-by":"publisher","first-page":"2176","DOI":"10.1002\/jmv.23707","volume":"85","author":"R Mancuso","year":"2013","unstructured":"Mancuso R, Saresella M, Hernis A, et al. Torque teno virus (TTV) in multiple sclerosis patients with different patterns of disease. J Med Virol. 2013;85(12):2176\u201383. \n                    https:\/\/doi.org\/10.1002\/jmv.23707\n                    \n                  . PubMed PMID: 23960011","journal-title":"J Med Virol"},{"issue":"8","key":"2233_CR5","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1080\/08880010290097396","volume":"19","author":"B Shiramizu","year":"2002","unstructured":"Shiramizu B, Yu Q, Hu N, et al. Investigation of TT virus in the etiology of pediatric acute lymphoblastic leukemia. Pediatr Hematol Oncol. 2002;19(8):543\u201351. \n                    https:\/\/doi.org\/10.1080\/08880010290097396\n                    \n                  . PubMed PMID: 12487829","journal-title":"Pediatr Hematol Oncol"},{"key":"2233_CR6","doi-asserted-by":"publisher","first-page":"33452","DOI":"10.1038\/srep33452","volume":"6","author":"J Kawada","year":"2016","unstructured":"Kawada J, Okuno Y, Torii Y, et al. Identification of Viruses in Cases of Pediatric Acute Encephalitis and Encephalopathy Using Next-Generation Sequencing. Sci Rep. 2016;6:33452. \n                    https:\/\/doi.org\/10.1038\/srep33452\n                    \n                  . PubMed PMID: 27625312; PubMed Central PMCID: PMCPMC5022051","journal-title":"Sci Rep"},{"issue":"24","key":"2233_CR7","doi-asserted-by":"publisher","first-page":"2327","DOI":"10.1056\/NEJMoa1803396","volume":"380","author":"MR Wilson","year":"2019","unstructured":"Wilson MR, Sample HA, Zorn KC, et al. Clinical Metagenomic Sequencing for Diagnosis of Meningitis and Encephalitis. N Engl J Med. 2019;380(24):2327\u201340. \n                    https:\/\/doi.org\/10.1056\/NEJMoa1803396\n                    \n                  . PMID: 31189036","journal-title":"N Engl J Med"}],"container-title":["Journal of Medical Case Reports"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13256-019-2233-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13256-019-2233-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13256-019-2233-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,22]],"date-time":"2020-09-22T23:11:07Z","timestamp":1600816267000},"score":1,"resource":{"primary":{"URL":"https:\/\/jmedicalcasereports.biomedcentral.com\/articles\/10.1186\/s13256-019-2233-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,24]]},"references-count":7,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["2233"],"URL":"https:\/\/doi.org\/10.1186\/s13256-019-2233-2","relation":{},"ISSN":["1752-1947"],"issn-type":[{"value":"1752-1947","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,24]]},"assertion":[{"value":"26 September 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 August 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 September 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study protocol was approved by the National Institute of Infectious Diseases in Japan (Approval No. 677) and was conducted according to the Declaration of Helsinki Principles.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consent was obtained from the patient\u2019s legal guardian for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors report no conflicts of interest. A funding agency had no role in the study design, data collection, and analysis, decision to publish, or preparation of the manuscript. No potential conflict of interest was reported by the authors.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"302"}}