{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T14:41:08Z","timestamp":1776696068851,"version":"3.51.2"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:00:00Z","timestamp":1721174400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Med Case Reports"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Escherichia coli is<\/jats:italic> a major human pathogen responsible for a broad range of clinical illnesses. It has been linked to endemic and epidemic nosocomial diseases caused by multidrug-resistant pathogens in Sudan as well as throughout the globe.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Case presentation<\/jats:title>\n                <jats:p>A 76-year-old African woman arrived at Saad Rashwan Medical Centre complaining of backaches and discomfort during urination. Throughout the preceding 5 years, the patient had recurrent urinary tract infections. Following overnight incubation at 37\u00a0\u00b0C, <jats:italic>Escherichia coli<\/jats:italic> was found in her midstream urine specimen on cysteine lactose electrolyte deficient agar media. Minimum inhibitory concentration (colorimetric\/turbidimetric method) was employed to test a wide range of antimicrobial drugs against this bacterial strain, and the results revealed significant multidrug resistance. QIAamp<jats:sup>\u00ae<\/jats:sup> DNA Mini Kit was used to obtain DNA Template from the purified <jats:italic>Escherichia coli<\/jats:italic> (Qiagen, Hilden, Germany). The bacterial whole-genome sequence was done by Novogene company (Hong Kong) using Illumina HiSeq 2500 (Illumina, San Diego, CA, USA), followed by whole genome reconstructions, and identification of antibiotic-resistant genes. Phylogenetic analysis revealed that our strain was related to the <jats:italic>Escherichia coli<\/jats:italic> DSM30083 ( genome sequence ID: CP033092.2) from the USA. Our strain possessed the following antimicrobial-resistant genes: aminoglycoside (<jats:italic>kdpE, baeR, cpxA, aadA5<\/jats:italic>), nitroimidazole (<jats:italic>msbA<\/jats:italic>), phosphonic acid (<jats:italic>mdtG<\/jats:italic>), tetracycline (<jats:italic>emrY<\/jats:italic>), macrolide, penam, tetracycline, (<jats:italic>evgA, TolC, H-NS<\/jats:italic>), fluoroquinolone, cephalosporin, glycylcycline, penam, tetracycline, rifamycin, phenicol antibiotic, disinfecting agents and antiseptics (<jats:italic>acrB; marA<\/jats:italic>), sulfonamide (<jats:italic>sul1<\/jats:italic>), macrolide (<jats:italic>Mrx<\/jats:italic>), cephalosporin, penam (<jats:italic>CTX-M-15<\/jats:italic>), carbapenem, cephalosporin, and penam (<jats:italic>OXA-1<\/jats:italic>).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study found that the isolated <jats:italic>Escherichia coli<\/jats:italic> strain had varied antimicrobial resistance genes on the basis of whole-genome sequencing and phenotypic resistance analyses. Whole-genome sequencing is critical for control and preventative methods to battle the growing threat of antimicrobial resistance. A larger investigation is recommended for improved generalization of results.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13256-024-04663-4","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T00:02:29Z","timestamp":1721174549000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Whole-genome sequencing of multidrug-resistant Escherichia coli causing urinary tract infection in an immunocompromised patient: a case report"],"prefix":"10.1186","volume":"18","author":[{"given":"Mohammed Yahya","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Babbiker Mohammed Taher","family":"Gorish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esraa Mohammed","family":"Alhaj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayasir Abd Elmoniem Abd","family":"Elrhim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shimaa Saifaldeen","family":"Siddig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisham N.","family":"Altayb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,17]]},"reference":[{"issue":"1","key":"4663_CR1","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1080\/1120009X.2017.1380395","volume":"29","author":"A Mazzariol","year":"2017","unstructured":"Mazzariol A, Bazaj A, Cornaglia G. 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Written informed consent was obtained from the patient for publication of this case report and any accompanying images, and a copy of the letter of consent is available to review by the editor-in-chief of this journal. All procedures in this study were carried out in conformity accordance with the rules and regulations specified in the Declaration of Helsinki that were applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publications"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"326"}}