{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T05:43:37Z","timestamp":1778910217812,"version":"3.51.4"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T00:00:00Z","timestamp":1437436800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T00:00:00Z","timestamp":1437436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Introduction<\/jats:title>\n                <jats:p>Stem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of implantation and track the migration of stem cells <jats:italic>in vivo<\/jats:italic> hampers the development of stem cell therapy and its clinical application.<\/jats:p>\n                <jats:p>In this report, we present data that demonstrate the feasibility of using the human sodium iodide symporter (<jats:italic>hNIS<\/jats:italic>) as a reporter gene for tracking neural stem cells (NSCs) after transplantation in the brain by using single-photon emission tomography\/computed tomography (SPECT\/CT) imaging.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>NSCs were isolated from the hippocampus of adult rats (Hipp-NSCs) and transduced with a lentiviral vector containing the hNIS gene. Hipp-NSCs expressing the hNIS (NIS-Hipp-NSCs) were characterized <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> after transplantation in the rat brain and imaged by using technetium-99m (<jats:sup>99m<\/jats:sup>Tc) and a small rodent SPECT\/CT apparatus. Comparisons were made between Hipp-NSCs and NIS-Hipp-NSCs, and statistical analysis was performed by using two-tailed Student\u2019s <jats:italic>t<\/jats:italic> test.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Our results show that the expression of the hNIS allows the repeated visualization of NSCs <jats:italic>in vivo<\/jats:italic> in the brain by using SPECT\/CT imaging and does not affect the ability of Hipp-NSCs to generate neuronal and glial cells <jats:italic>in vitro<\/jats:italic> and<jats:italic> in vivo<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>These data support the use of the hNIS as a reporter gene for non-invasive imaging of NSCs in the brain. The repeated, non-invasive tracking of implanted cells will accelerate the development of effective stem cell therapies for traumatic brain injury and other types of central nervous system injury.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13287-015-0129-7","type":"journal-article","created":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T16:48:02Z","timestamp":1437410882000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of a novel imaging system for cell therapy in the brain"],"prefix":"10.1186","volume":"6","author":[{"given":"Maria-Adelaide","family":"Micci","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debbie R.","family":"Boone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret A.","family":"Parsley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingna","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Patrikeev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massoud","family":"Motamedi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen L.","family":"Hellmich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,7,21]]},"reference":[{"key":"129_CR1","doi-asserted-by":"publisher","first-page":"14069","DOI":"10.1073\/pnas.0507063102","volume":"102","author":"BJ Cummings","year":"2005","unstructured":"Cummings BJ, Uchida N, Tamaki SJ, Salazar DL, Hooshmand M, Summers R, et al. 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