{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T23:03:08Z","timestamp":1772751788551,"version":"3.50.1"},"reference-count":60,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T00:00:00Z","timestamp":1441756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Introduction<\/jats:title>\n                <jats:p>Due to their immunomodulatory properties, mesenchymal stromal cells (MSCs) have been used for auto-immune disease treatment. Crohn disease (CD) and ulcerative colitis are two major inflammatory bowel diseases (IBDs), resulting from pathological immune responses to environmental or microbial antigens. Preclinical and clinical studies have suggested that MSC-based cellular therapy hold promising potential for IBD treatment. However, open issues include the selection of the proper cell dose, the source and the optimal route of administration of MSCs for more effective results. Platelet lysate has gained clinical interest due to its efficacy in accelerating wound healing. Thus, we propose to combine the administration of MSCs with a human umbilical cord blood-derived platelet lysate (hCBPL) as a novel strategy to improve MSC-based therapy for IBD resolution.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Colitis was induced in 8-week-old C57BL\/6J mice by daily oral administration of dextran sulphate sodium (DSS) (1.5 % w\/v in tap water) for 9 days. MSCs were isolated from adipose tissue of CD patients (adCD-MSCs), expanded in proliferation medium, resuspended in hCBPL or PBS and administrated via enema for three times (1 \u00d7 10<jats:sup>6<\/jats:sup> cells\/mouse\/time) every other day starting on day +7 from DSS induction. The colitis evolution was evaluated by daily monitoring of body weight, stool consistency and bleeding. Histopathological analysis was performed. Inflammatory cytokine plasma levels were determined. adCD-MSCs stained with lipophilic membrane dye Nile Red, were injected in DSS mice as described above. Colon section of mice sacrificed 24 hours after last cell administration, were analyzed by confocal microscopy.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We found that adCD-MSCs could be easily isolated and expanded from CD patients. Upon injection, adCD-MSCs exerted a therapeutic effect on DSS-induced colitis. Moreover, hCBPL increased adCD-MSCs efficacy by significantly reducing colitis scores, extension of the colon inflamed area and plasma levels of inflammatory mediators. Finally, Nile Red staining of MSCs is very efficient, stable and does not impair their vitality and function. Nile Red-labelling was clearly detected in the colitic area of adCD-MSCs injected mice and it was significantly brighter in the colon sections of mice that had received adCD-MSCs\/hCBPL.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>In summary, with this study we propose a novel and promising adCD-MSC\/hCBPL-based therapy for refractory IBDs.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13287-015-0166-2","type":"journal-article","created":{"date-parts":[[2015,9,10]],"date-time":"2015-09-10T12:09:20Z","timestamp":1441886960000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Human cord blood-derived platelet lysate enhances the therapeutic activity of adipose-derived mesenchymal stromal cells isolated from Crohn\u2019s disease patients in a mouse model of colitis"],"prefix":"10.1186","volume":"6","author":[{"given":"Dorian","family":"Forte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena","family":"Ciciarello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Chiara","family":"Valerii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigia","family":"De Fazio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Cavazza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosaria","family":"Giordano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Parazzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenza","family":"Lazzari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio","family":"Laureti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Rizzello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Cavo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Curti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto M.","family":"Lemoli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enzo","family":"Spisni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Catani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,9,9]]},"reference":[{"key":"166_CR1","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1002\/jor.1100090504","volume":"9","author":"AI Caplan","year":"1991","unstructured":"Caplan AI. Mesenchymal stem cells. J Orthop Res. 1991;9:641\u201350.","journal-title":"J Orthop Res."},{"key":"166_CR2","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1126\/science.284.5411.143","volume":"284","author":"MF Pittenger","year":"1999","unstructured":"Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143\u20137.","journal-title":"Science."},{"key":"166_CR3","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/nature00870","volume":"418","author":"Y Jiang","year":"2002","unstructured":"Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 2002;418:41\u20139.","journal-title":"Nature."},{"key":"166_CR4","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1080\/14653240310003611","volume":"5","author":"K Le Blanc","year":"2003","unstructured":"Le Blanc K. Immunomodulatory effects of fetal and adult mesenchymal stem cells. Cytotherapy. 2003;5:485\u20139.","journal-title":"Cytotherapy."},{"key":"166_CR5","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1196\/annals.1392.025","volume":"1106","author":"WE Fibbe","year":"2007","unstructured":"Fibbe WE, Nauta AJ, Roelofs H. Modulation of immune responses by mesenchymal stem cells. Ann N Y Acad Sci. 2007;1106:272\u20138.","journal-title":"Ann N Y Acad Sci."},{"key":"166_CR6","doi-asserted-by":"publisher","first-page":"3722","DOI":"10.1182\/blood-2002-07-2104","volume":"101","author":"M Krampera","year":"2003","unstructured":"Krampera M, Glennie S, Dyson J, Scott D, Laylor R, Simpson E, et al. Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-specific T cells to their cognate peptide. Blood. 2003;101:3722\u20139.","journal-title":"Blood."},{"key":"166_CR7","doi-asserted-by":"publisher","first-page":"2566","DOI":"10.1002\/eji.200636416","volume":"36","author":"A Uccelli","year":"2006","unstructured":"Uccelli A, Moretta L, Pistoia V. Immunoregulatory function of mesenchymal stem cells. Eur J Immunol. 2006;36:2566\u201373.","journal-title":"Eur J Immunol."},{"key":"166_CR8","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1002\/jcp.21200","volume":"213","author":"AI Caplan","year":"2007","unstructured":"Caplan AI. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol. 2007;213:341\u20137.","journal-title":"J Cell Physiol."},{"key":"166_CR9","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1080\/14653240903080367","volume":"11","author":"JA Kode","year":"2009","unstructured":"Kode JA, Mukherjee S, Joglekar MV, Hardikar AA. Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy. 2009;11:377\u201391.","journal-title":"Cytotherapy."},{"key":"166_CR10","doi-asserted-by":"publisher","first-page":"576","DOI":"10.3109\/14653249.2010.507330","volume":"12","author":"DJ Prockop","year":"2010","unstructured":"Prockop DJ, Brenner M, Fibbe WE, Horwitz E, Le Blanc K, Phinney DG, et al. Defining the risks of mesenchymal stromal cell therapy. Cytotherapy. 2010;12:576\u20138.","journal-title":"Cytotherapy."},{"key":"166_CR11","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1002\/stem.459","volume":"28","author":"J Tolar","year":"2010","unstructured":"Tolar J, Le Blanc K, Keating A, Blazar BR. Concise review: hitting the right spot with mesenchymal stromal cells. Stem Cells. 2010;28:1446\u201355.","journal-title":"Stem Cells."},{"key":"166_CR12","doi-asserted-by":"publisher","first-page":"1579","DOI":"10.1016\/S0140-6736(08)60690-X","volume":"371","author":"K Le Blanc","year":"2008","unstructured":"Le Blanc K, Frassoni F, Ball L, Locatelli F, Roelofs H, Lewis I, et al. Developmental Committee of the European Group for Blood and Marrow Transplantation. Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study. Lancet. 2008;371:1579\u201386.","journal-title":"Lancet"},{"key":"166_CR13","doi-asserted-by":"publisher","first-page":"1439","DOI":"10.1016\/S0140-6736(04)16104-7","volume":"363","author":"K Le Blanc","year":"2004","unstructured":"Le Blanc K, Rasmusson I, Sundberg B, G\u00f6therstr\u00f6m C, Hassan M, Uzunel M, et al. Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells. Lancet. 2004;363:1439\u201341.","journal-title":"Lancet."},{"key":"166_CR14","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1016\/j.bbmt.2011.09.003","volume":"18","author":"F Baron","year":"2012","unstructured":"Baron F, Storb R. Mesenchymal stromal cells: a new tool against graft-versus-host disease? Biol Blood Marrow Transplant. 2012;18:822\u201340.","journal-title":"Biol Blood Marrow Transplant."},{"key":"166_CR15","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.beha.2011.01.002","volume":"24","author":"F Dazzi","year":"2011","unstructured":"Dazzi F, Krampera M. Mesenchymal stem cells and autoimmune diseases. Best Pract Res Clin Haematol. 2011;24:49\u201357.","journal-title":"Best Pract Res Clin Haematol."},{"key":"166_CR16","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1111\/bjh.12545","volume":"163","author":"LM Ball","year":"2013","unstructured":"Ball LM, Bernardo ME, Roelofs H, van Tol MJ, Contoli B, Zwaginga JJ, et al. Multiple infusions of mesenchymal stromal cells induce sustained remission in children with steroid-refractory, grade III-IV acute graft-versus-host disease. Br J Haematol. 2013;163:501\u20139.","journal-title":"Br J Haematol."},{"key":"166_CR17","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1038\/nri2340","volume":"8","author":"JH Cho","year":"2008","unstructured":"Cho JH. The genetics and immunopathogenesis of inflammatory bowel disease. Nat Rev Immunol. 2008;8:458\u201366.","journal-title":"Nat Rev Immunol."},{"key":"166_CR18","doi-asserted-by":"publisher","first-page":"1398","DOI":"10.1056\/NEJM199905063401804","volume":"340","author":"DH Present","year":"1999","unstructured":"Present DH, Rutgeerts P, Targan S, Hanauer SB, Mayer L, van Hogezand RA, et al. Infliximab for the treatment of fistulas in patients with Crohn\u2019s disease. N Engl J Med. 1999;340:1398\u2013405.","journal-title":"N Engl J Med."},{"key":"166_CR19","doi-asserted-by":"publisher","first-page":"876","DOI":"10.1056\/NEJMoa030815","volume":"350","author":"BE Sands","year":"2004","unstructured":"Sands BE, Anderson FH, Bernstein CN, Chey WY, Feagan BG, Fedorak RN, et al. Infliximab maintenance therapy for fistulizing Crohn\u2019s disease. N Engl J Med. 2004;350:876\u201385.","journal-title":"N Engl J Med."},{"key":"166_CR20","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1053\/j.gastro.2008.11.041","volume":"136","author":"MA Gonz\u00e1lez","year":"2009","unstructured":"Gonz\u00e1lez MA, Gonzalez-Rey E, Rico L, B\u00fcscher D, Delgado M. Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. Gastroenterology. 2009;136:978\u201389.","journal-title":"Gastroenterology."},{"key":"166_CR21","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1136\/gut.2008.168534","volume":"58","author":"E Gonzalez-Rey","year":"2009","unstructured":"Gonzalez-Rey E, Anderson P, Gonz\u00e1lez MA, Rico L, B\u00fcscher D, Delgado M. Human adult stem cells derived from adipose tissue protect against experimental colitis and sepsis. Gut. 2009;58:929\u201339.","journal-title":"Gut."},{"key":"166_CR22","doi-asserted-by":"publisher","first-page":"7787","DOI":"10.4049\/jimmunol.0902318","volume":"183","author":"Q Zhang","year":"2009","unstructured":"Zhang Q, Shi S, Liu Y, Uyanne J, Shi Y, Shi S, et al. Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis. J Immunol. 2009;183:7787\u201398.","journal-title":"J Immunol."},{"key":"166_CR23","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.3727\/096368910X557245","volume":"20","author":"L Liang","year":"2011","unstructured":"Liang L, Dong C, Chen X, Fang Z, Xu J, Liu M, et al. Human umbilical cord mesenchymal stem cells ameliorate mice trinitrobenzene sulfonic acid (TNBS)-induced colitis. Cell Transplant. 2011;20:1395\u2013408.","journal-title":"Cell Transplant."},{"key":"166_CR24","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1002\/stem.698","volume":"29","author":"M Duijvestein","year":"2011","unstructured":"Duijvestein M, Wildenberg ME, Welling MM, Hennink S, Molendijk I, van Zuylen VL, et al. Pretreatment with interferon-\u03b3 enhances the therapeutic activity of mesenchymal stromal cells in animal models of colitis. Stem Cells. 2011;29:1549\u201358.","journal-title":"Stem Cells."},{"key":"166_CR25","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1038\/cmi.2012.40","volume":"9","author":"H Fan","year":"2012","unstructured":"Fan H, Zhao G, Liu L, Liu F, Gong W, Liu X, et al. Pre-treatment with IL-1\u03b2 enhances the efficacy of MSC transplantation in DSS-induced colitis. Cell Mol Immunol. 2012;9:473\u201381.","journal-title":"Cell Mol Immunol."},{"key":"166_CR26","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1007\/s00384-003-0490-3","volume":"18","author":"D Garc\u00eda-Olmo","year":"2003","unstructured":"Garc\u00eda-Olmo D, Garc\u00eda-Arranz M, Garc\u00eda LG, Cuellar ES, Blanco IF, Prianes LA, et al. Autologous stem cell transplantation for treatment of rectovaginal fistula in perianal Crohn\u2019s disease: a new cell-based therapy. Int J Colorectal Dis. 2003;18:451\u20134.","journal-title":"Int J Colorectal Dis."},{"key":"166_CR27","doi-asserted-by":"publisher","first-page":"1416","DOI":"10.1007\/s10350-005-0052-6","volume":"48","author":"D Garc\u00eda-Olmo","year":"2005","unstructured":"Garc\u00eda-Olmo D, Garc\u00eda-Arranz M, Herreros D, Pascual I, Peiro C, Rodr\u00edguez-Montes JA. A phase I clinical trial of the treatment of Crohn\u2019s fistula by adipose mesenchymal stem cell transplantation. Dis Colon Rectum. 2005;48:1416\u201323.","journal-title":"Dis Colon Rectum."},{"key":"166_CR28","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1007\/DCR.0b013e3181973487","volume":"52","author":"D Garcia-Olmo","year":"2009","unstructured":"Garcia-Olmo D, Herreros D, Pascual I, Pascual JA, Del-Valle E, Zorrilla J, et al. Expanded adipose-derived stem cells for the treatment of complex perianal fistula: a phase II clinical trial. Dis Colon Rectum. 2009;52:79\u201386.","journal-title":"Dis Colon Rectum."},{"key":"166_CR29","doi-asserted-by":"publisher","first-page":"1662","DOI":"10.1136\/gut.2010.215152","volume":"59","author":"M Duijvestein","year":"2010","unstructured":"Duijvestein M, Vos AC, Roelofs H, Wildenberg ME, Wendrich BB, Verspaget HW, et al. Autologous bone marrow-derived mesenchymal stromal cell treatment for refractory luminal Crohn\u2019s disease: results of a phase I study. Gut. 2010;59:1662\u20139.","journal-title":"Gut."},{"key":"166_CR30","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1136\/gut.2010.214841","volume":"60","author":"R Ciccocioppo","year":"2011","unstructured":"Ciccocioppo R, Bernardo ME, Sgarella A, Maccario R, Avanzini MA, Ubezio C, et al. Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn\u2019s disease. Gut. 2011;60:788\u201398.","journal-title":"Gut."},{"key":"166_CR31","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.cgh.2013.06.021","volume":"12","author":"GM Forbes","year":"2014","unstructured":"Forbes GM, Sturm MJ, Leong RW, Sparrow MP, Segarajasingam D, Cummins AG, et al. A phase 2 study of allogeneic mesenchymal stromal cells for luminal Crohn\u2019s disease refractory to biologic therapy. Clin Gastroenterol Hepatol. 2014;12:64\u201371.","journal-title":"Clin Gastroenterol Hepatol."},{"key":"166_CR32","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1517\/14712598.6.6.567","volume":"6","author":"AM Parker","year":"2006","unstructured":"Parker AM, Katz AJ. Adipose-derived stem cells for the regeneration of damaged tissues. Expert Opin Biol Ther. 2006;6:567\u201378.","journal-title":"Expert Opin Biol Ther."},{"key":"166_CR33","doi-asserted-by":"crossref","unstructured":"Puissant B, Barreau C, Bourin P, Clavel C, Corre J, Bousquet C, et al. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells. Br J Haematol. 2005;129:118\u201329.","DOI":"10.1111\/j.1365-2141.2005.05409.x"},{"key":"166_CR34","doi-asserted-by":"crossref","first-page":"695","DOI":"10.3892\/ijmm.2014.1821","volume":"34","author":"X Li","year":"2014","unstructured":"Li X, Bai J, Ji X, Li R, Xuan Y, Wang Y. Comprehensive characterization of four different populations of human mesenchymal stem cells as regards their immune properties, proliferation and differentiation. Int J Mol Med. 2014;34:695\u2013704.","journal-title":"Int J Mol Med."},{"key":"166_CR35","doi-asserted-by":"publisher","first-page":"455","DOI":"10.5966\/sctm.2012-0184","volume":"2","author":"SM Melief","year":"2013","unstructured":"Melief SM, Zwaginga JJ, Fibbe WE, Roelofs H. Adipose tissue-derived multipotent stromal cells have a higher immunomodulatory capacity than their bone marrow-derived counterparts. Stem Cells Transl Med. 2013;2:455\u201363.","journal-title":"Stem Cells Transl Med."},{"key":"166_CR36","doi-asserted-by":"publisher","first-page":"2582","DOI":"10.1634\/stemcells.2006-0228","volume":"24","author":"R Ya\u00f1ez","year":"2006","unstructured":"Ya\u00f1ez R, Lamana ML, Garc\u00eda-Castro J, Colmenero I, Ram\u00edrez M, Bueren JA. Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the graft-versus-host disease. Stem Cells. 2006;24:2582\u201391.","journal-title":"Stem Cells."},{"key":"166_CR37","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/scrt97","volume":"3","author":"N Ben Azouna","year":"2012","unstructured":"Ben Azouna N, Jenhani F, Regaya Z, Berraeis L, Ben Othman T, Ducrocq E, et al. Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum. Stem Cell Res Ther. 2012;3:6\u201318.","journal-title":"Stem Cell Res Ther."},{"key":"166_CR38","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1002\/jcp.20911","volume":"211","author":"ME Bernardo","year":"2007","unstructured":"Bernardo ME, Avanzini MA, Perotti C, Cometa AM, Moretta A, Lenta E, et al. Optimization of in vitro expansion of human multipotent mesenchymal stromal cells for cell-therapy approaches: further insights in the search for a fetal calf serum substitute. J Cell Physiol. 2007;211:121\u201330.","journal-title":"J Cell Physiol."},{"key":"166_CR39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068984","volume":"8","author":"SM Jonsdottir-Buch","year":"2013","unstructured":"Jonsdottir-Buch SM, Lieder R, Sigurjonsson OE. Platelet lysates produced from expired platelet concentrates support growth and osteogenic differentiation of mesenchymal stem cells. PLoS One. 2013;8, e68984.","journal-title":"PLoS One."},{"key":"166_CR40","doi-asserted-by":"publisher","first-page":"638","DOI":"10.1016\/S1079-2104(98)90029-4","volume":"85","author":"RE Marx","year":"1998","unstructured":"Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:638\u201346.","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol Endod."},{"key":"166_CR41","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.joms.2003.12.003","volume":"62","author":"RE Marx","year":"2004","unstructured":"Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg. 2004;62:489\u201396.","journal-title":"J Oral Maxillofac Surg."},{"key":"166_CR42","doi-asserted-by":"publisher","first-page":"1502","DOI":"10.1097\/01.PRS.0000138251.07040.51","volume":"114","author":"BL Eppley","year":"2004","unstructured":"Eppley BL, Woodell JE, Higgins J. Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg. 2004;114:1502\u20138.","journal-title":"Plast Reconstr Surg."},{"key":"166_CR43","doi-asserted-by":"publisher","first-page":"2051","DOI":"10.3748\/wjg.v20.i8.2051","volume":"20","author":"L De Fazio","year":"2014","unstructured":"De Fazio L, Cavazza E, Spisni E, Strillacci A, Centanni M, Candela M, et al. Longitudinal analysis of inflammation and microbiota dynamics in a model of mild chronic dextran sulfate sodium-induced colitis in mice. World J Gastroenterol. 2014;20:2051\u201361.","journal-title":"World J Gastroenterol."},{"key":"166_CR44","first-page":"1","volume":"20","author":"E Montelatici","year":"2014","unstructured":"Montelatici E, Baluce B, Ragni E, Lavazza C, Parazzi V, Mazzola R, et al. Defining the identity of human adipose derived mesenchymal stem cells. Biochem Cell Biol. 2014;20:1\u20139.","journal-title":"Biochem Cell Biol."},{"key":"166_CR45","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.1089\/scd.2012.0432","volume":"22","author":"M Ciciarello","year":"2013","unstructured":"Ciciarello M, Zini R, Rossi L, Salvestrini V, Ferrari D, Manfredini R, et al. Extracellular purines promote the differentiation of human bone marrow-derived mesenchymal stem cells to the osteogenic and adipogenic lineages. Stem Cells Dev. 2013;22:1097\u2013111.","journal-title":"Stem Cells Dev."},{"key":"166_CR46","first-page":"843","volume":"21","author":"G Sirchia","year":"1998","unstructured":"Sirchia G, Rebulla P, Mozzi F, Lecchi L, Lazzari L, Ratti I. A quality system for placental blood banking. Bone Marrow Transplant. 1998;21:843\u20137.","journal-title":"Bone Marrow Transplant."},{"key":"166_CR47","doi-asserted-by":"publisher","first-page":"549","DOI":"10.3109\/09537104.2010.514626","volume":"21","author":"V Parazzi","year":"2010","unstructured":"Parazzi V, Lazzari L, Rebulla P. Platelet gel from cord blood: a novel tool for tissue engineering. Platelets. 2010;21:549\u201354.","journal-title":"Platelets."},{"key":"166_CR48","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1046\/j.1365-2249.1999.00878.x","volume":"116","author":"F Obermeier","year":"1999","unstructured":"Obermeier F, Kojouharoff G, Hans W, Scholmerich J, Gross V, Falk W. Interferon-gamma (IFNgamma)-and tumour necrosis factor (TNF)-induced nitric oxide as toxic effector molecule in chronic dextran sulphate sodium (DSS)-induced colitis in mice. Clin Exp Immunol. 1999;116:238\u201345.","journal-title":"Clin Exp Immunol."},{"key":"166_CR49","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.jcyt.2013.02.006","volume":"15","author":"P Bourin","year":"2013","unstructured":"Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal\/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013;15:641\u20138.","journal-title":"Cytotherapy."},{"key":"166_CR50","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1083\/jcb.100.3.965","volume":"100","author":"P Greenspan","year":"1985","unstructured":"Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985;100:965\u201373.","journal-title":"J Cell Biol."},{"key":"166_CR51","first-page":"s235","volume":"12","author":"L Rosso","year":"2014","unstructured":"Rosso L, Parazzi V, Damarco F, Righi I, Santambrogio L, Rebulla P, et al. Pleural tissue repair with cord blood platelet gel. Blood Transfus. 2014;12:s235\u201342.","journal-title":"Blood Transfus."},{"key":"166_CR52","doi-asserted-by":"publisher","first-page":"1858","DOI":"10.1089\/scd.2013.0567","volume":"23","author":"V Ulivi","year":"2014","unstructured":"Ulivi V, Tasso R, Cancedda R, Descalzi F. Mesenchymal stem cells paracrine activity is modulated by platelet lysate: induction of an inflammatory response and secretion of factors maintaining macrophages in a pro-inflammatory phenotype. Stem Cells Dev. 2014;23:1858\u201369.","journal-title":"Stem Cells Dev."},{"key":"166_CR53","doi-asserted-by":"publisher","first-page":"916","DOI":"10.5966\/sctm.2013-0030","volume":"2","author":"C Linard","year":"2013","unstructured":"Linard C, Busson E, Holler V, Strup-Perrot C, Lacave-Lapalun JV, Lhomme B, et al. Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs. Stem Cells Transl Med. 2013;2:916\u201327.","journal-title":"Stem Cells Transl Med."},{"key":"166_CR54","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1002\/ibd.20753","volume":"15","author":"P Alex","year":"2009","unstructured":"Alex P, Zachos NC, Nguyen T, Gonzales L, Chen TE, Conklin LS, et al. Distinct cytokine patterns identified from multiplex profiles of murine DSS and TNBS-induced colitis. Inflamm Bowel Dis. 2009;15:341\u201352.","journal-title":"Inflamm Bowel Dis."},{"key":"166_CR55","doi-asserted-by":"publisher","first-page":"5308","DOI":"10.1016\/j.biomaterials.2012.04.007","volume":"33","author":"MB Murphy","year":"2012","unstructured":"Murphy MB, Blashki D, Buchanan RM, Yazdi IK, Ferrari M, Simmons PJ, et al. Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation. Biomaterials. 2012;33:5308\u201316.","journal-title":"Biomaterials."},{"key":"166_CR56","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1016\/j.lfs.2008.09.016","volume":"83","author":"F Tanaka","year":"2008","unstructured":"Tanaka F, Tominaga K, Ochi M, Tanigawa T, Watanabe T, Fujiwara Y, et al. Exogenous administration of mesenchymal stem cells ameliorates dextran sulfate sodium-induced colitis via anti-inflammatory action in damaged tissue in rats. Life Sci. 2008;83:771\u20139.","journal-title":"Life Sci."},{"key":"166_CR57","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1097\/TP.0b013e3181a9f0a7","volume":"88","author":"Y Wei","year":"2009","unstructured":"Wei Y, Nie Y, Lai J, Wan YJ, Li Y. Comparison of the population capacity of hematopoietic and mesenchymal stem cells in experimental colitis rat model. Transplantation. 2009;88:42\u20138.","journal-title":"Transplantation."},{"key":"166_CR58","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033360","volume":"7","author":"MT Castelo-Branco","year":"2012","unstructured":"Castelo-Branco MT, Soares ID, Lopes DV, Buongusto F, Martinusso CA, Do Rosario Jr A, et al. Intraperitoneal but not intravenous cryopreserved mesenchymal stromal cells home to the inflamed colon and ameliorate experimental colitis. PLoS One. 2012;7:e33360.","journal-title":"PLoS One."},{"key":"166_CR59","doi-asserted-by":"publisher","first-page":"4977","DOI":"10.1007\/s11033-014-3364-6","volume":"41","author":"C Wang","year":"2014","unstructured":"Wang C, Chen J, Sun L, Liu Y. TGF-beta signaling-dependent alleviation of dextran sulfate sodium-induced colitis by mesenchymal stem cell transplantation. Mol Biol Rep. 2014;41:4977\u201383.","journal-title":"Mol Biol Rep."},{"key":"166_CR60","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/j.intimp.2014.07.030","volume":"22","author":"AG Abdel Salam","year":"2014","unstructured":"Abdel Salam AG, Ata HM, Salman TM, Rashed LA, Sabry D, Schaalan MF. Potential therapeutic utility of mesenchymal stem cells in inflammatory bowel disease in mice. Int Immunopharmacol. 2014;22:515\u201321.","journal-title":"Int Immunopharmacol."}],"container-title":["Stem Cell Research &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-015-0166-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13287-015-0166-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-015-0166-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-015-0166-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T06:20:24Z","timestamp":1637043624000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-015-0166-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,9]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["166"],"URL":"https:\/\/doi.org\/10.1186\/s13287-015-0166-2","relation":{},"ISSN":["1757-6512"],"issn-type":[{"value":"1757-6512","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,9]]},"assertion":[{"value":"30 March 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 April 2015","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 August 2015","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 September 2015","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"170"}}