{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:23:22Z","timestamp":1740158602760,"version":"3.37.3"},"reference-count":64,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T00:00:00Z","timestamp":1470268800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T00:00:00Z","timestamp":1470268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["106-YS.06-2013.37."],"award-info":[{"award-number":["106-YS.06-2013.37."]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Endothelial progenitor cell (EPC) transplantation is a promising therapy for ischemic diseases such as ischemic myocardial infarction and hindlimb ischemia. However, limitation of EPC sources remains a major obstacle. Direct reprogramming has become a powerful tool to produce EPCs from fibroblasts. Some recent efforts successfully directly reprogrammed human fibroblasts into functional EPCs; however, the procedure efficacy was low. This study therefore aimed to improve the efficacy of direct reprogramming of human fibroblasts to functional EPCs.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Human fibroblasts isolated from foreskin were directly reprogrammed into EPCs by viral ETV2 transduction. Reprogramming efficacy was improved by culturing transduced fibroblasts in hypoxia conditions (5\u00a0% oxygen). Phenotype analyses confirmed that single-factor ETV2 transduction successfully reprogrammed dermal fibroblasts into functional EPCs.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Hypoxia treatment during the reprogramming procedure increased the efficacy of reprogramming from 1.21\u2009\u00b1\u20090.61\u00a0% in normoxia conditions to 7.52\u2009\u00b1\u20092.31\u00a0% in hypoxia conditions. Induced EPCs in hypoxia conditions exhibited functional EPC phenotypes similar to those in normoxia conditions, such as expression of CD31 and VEGFR2, and expressed endothelial gene profiles similar to human umbilical vascular endothelial cells. These cells also formed capillary-like networks in vitro.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our study demonstrates a new simple method to increase the reprogramming efficacy of human fibroblasts to EPCs using ETV2 and hypoxia.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13287-016-0368-2","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T00:44:42Z","timestamp":1470271482000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Significant improvement of direct reprogramming efficacy of fibroblasts into progenitor endothelial cells by ETV2 and hypoxia"],"prefix":"10.1186","volume":"7","author":[{"given":"Phuc","family":"Van Pham","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngoc Bich","family":"Vu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoa Trong","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oanh Thuy","family":"Huynh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai Thi-Hoang","family":"Truong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,8,4]]},"reference":[{"issue":"7","key":"368_CR1","doi-asserted-by":"publisher","first-page":"625","DOI":"10.2217\/bmm.15.34","volume":"9","author":"T Bogoslovsky","year":"2015","unstructured":"Bogoslovsky T, et al. Preservation and enumeration of endothelial progenitor and endothelial cells from peripheral blood for clinical trials. Biomark Med. 2015;9(7):625\u201337.","journal-title":"Biomark Med"},{"issue":"2","key":"368_CR2","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1002\/cyto.b.20085","volume":"70","author":"RG Shaffer","year":"2006","unstructured":"Shaffer RG, et al. Flow cytometric measurement of circulating endothelial cells: the effect of age and peripheral arterial disease on baseline levels of mature and progenitor populations. Cytometry B Clin Cytom. 2006;70(2):56\u201362.","journal-title":"Cytometry B Clin Cytom"},{"issue":"6","key":"368_CR3","doi-asserted-by":"publisher","first-page":"e0129935","DOI":"10.1371\/journal.pone.0129935","volume":"10","author":"D Tasev","year":"2015","unstructured":"Tasev D, et al. Long-term expansion in platelet lysate increases growth of peripheral blood-derived endothelial-colony forming cells and their growth factor-induced sprouting capacity. PLoS One. 2015;10(6):e0129935.","journal-title":"PLoS One"},{"issue":"6","key":"368_CR4","doi-asserted-by":"publisher","first-page":"e66734","DOI":"10.1371\/journal.pone.0066734","volume":"8","author":"E Colombo","year":"2013","unstructured":"Colombo E, et al. Comparison of fibronectin and collagen in supporting the isolation and expansion of endothelial progenitor cells from human adult peripheral blood. PLoS One. 2013;8(6):e66734.","journal-title":"PLoS One"},{"issue":"5302","key":"368_CR5","doi-asserted-by":"publisher","first-page":"964","DOI":"10.1126\/science.275.5302.964","volume":"275","author":"T Asahara","year":"1997","unstructured":"Asahara T, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997;275(5302):964\u20136.","journal-title":"Science"},{"key":"368_CR6","unstructured":"D\u2019Avola D, et al. Phase 1\u20132 pilot clinical trial in patients with decompensated liver cirrhosis treated with bone marrow-derived endothelial progenitor cells. Transl Res. 2016;S1931-5244(16)00063-3."},{"issue":"4","key":"368_CR7","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1159\/000438956","volume":"22","author":"P Donndorf","year":"2015","unstructured":"Donndorf P, et al. Mobilization of bone marrow-derived endothelial progenitor cells following Finnish sauna: a pilot study. Forsch Komplementmed. 2015;22(4):246\u201350.","journal-title":"Forsch Komplementmed"},{"issue":"11","key":"368_CR8","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1111\/cns.12447","volume":"21","author":"YY Bai","year":"2015","unstructured":"Bai YY, et al. Bone marrow endothelial progenitor cell transplantation after ischemic stroke: an investigation into its possible mechanism. CNS Neurosci Ther. 2015;21(11):877\u201386.","journal-title":"CNS Neurosci Ther"},{"key":"368_CR9","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1016\/j.ijcard.2015.10.110","volume":"203","author":"SW Kim","year":"2016","unstructured":"Kim SW, et al. Therapeutic effects of late outgrowth endothelial progenitor cells or mesenchymal stem cells derived from human umbilical cord blood on infarct repair. Int J Cardiol. 2016;203:498\u2013507.","journal-title":"Int J Cardiol."},{"issue":"9","key":"368_CR10","doi-asserted-by":"publisher","first-page":"2020","DOI":"10.1161\/ATVBAHA.115.306142","volume":"35","author":"J Kim","year":"2015","unstructured":"Kim J, et al. BMP9 induces cord blood-derived endothelial progenitor cell differentiation and ischemic neovascularization via ALK1. Arterioscler Thromb Vasc Biol. 2015;35(9):2020\u201331.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"10","key":"368_CR11","doi-asserted-by":"publisher","first-page":"2552","DOI":"10.1007\/s10439-015-1301-z","volume":"43","author":"EB Peters","year":"2015","unstructured":"Peters EB, et al. Umbilical cord blood-derived mononuclear cells exhibit pericyte-like phenotype and support network formation of endothelial progenitor cells in vitro. Ann Biomed Eng. 2015;43(10):2552\u201368.","journal-title":"Ann Biomed Eng"},{"issue":"2","key":"368_CR12","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1007\/s10561-011-9262-4","volume":"13","author":"PV Phuc","year":"2012","unstructured":"Phuc PV, et al. Isolation of three important types of stem cells from the same samples of banked umbilical cord blood. Cell Tissue Bank. 2012;13(2):341\u201351.","journal-title":"Cell Tissue Bank"},{"issue":"5","key":"368_CR13","first-page":"548","volume":"122","author":"CH Hu","year":"2009","unstructured":"Hu CH, et al. Human umbilical cord-derived endothelial progenitor cells promote growth cytokines-mediated neorevascularization in rat myocardial infarction. Chin Med J (Engl). 2009;122(5):548\u201355.","journal-title":"Chin Med J (Engl)"},{"issue":"5\u20136","key":"368_CR14","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1111\/j.1440-1681.2010.05347.x","volume":"37","author":"CH Hu","year":"2010","unstructured":"Hu CH, et al. Expanded human cord blood-derived endothelial progenitor cells salvage infarcted myocardium in rats with acute myocardial infarction. Clin Exp Pharmacol Physiol. 2010;37(5\u20136):551\u20136.","journal-title":"Clin Exp Pharmacol Physiol"},{"issue":"1","key":"368_CR15","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/s00395-007-0685-9","volume":"103","author":"A Schuh","year":"2008","unstructured":"Schuh A, et al. Transplantation of endothelial progenitor cells improves neovascularization and left ventricular function after myocardial infarction in a rat model. Basic Res Cardiol. 2008;103(1):69\u201377.","journal-title":"Basic Res Cardiol"},{"issue":"7","key":"368_CR16","doi-asserted-by":"publisher","first-page":"3422","DOI":"10.1073\/pnas.97.7.3422","volume":"97","author":"C Kalka","year":"2000","unstructured":"Kalka C, et al. Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci U S A. 2000;97(7):3422\u20137.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"368_CR17","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1161\/01.CIR.0000046450.89986.50","volume":"107","author":"A Kawamoto","year":"2003","unstructured":"Kawamoto A, et al. Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia. Circulation. 2003;107(3):461\u20138.","journal-title":"Circulation"},{"issue":"10","key":"368_CR18","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1634\/stemcells.2004-0340","volume":"23","author":"W Suh","year":"2005","unstructured":"Suh W, et al. Transplantation of endothelial progenitor cells accelerates dermal wound healing with increased recruitment of monocytes\/macrophages and neovascularization. Stem Cells. 2005;23(10):1571\u20138.","journal-title":"Stem Cells"},{"issue":"7","key":"368_CR19","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1177\/1933719113518983","volume":"21","author":"SK Cha","year":"2014","unstructured":"Cha SK, et al. Effect of human endothelial progenitor cell (EPC)- or mouse vascular endothelial growth factor-derived vessel formation on the survival of vitrified\/warmed mouse ovarian grafts. Reprod Sci. 2014;21(7):859\u201368.","journal-title":"Reprod Sci"},{"issue":"1","key":"368_CR20","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1089\/nat.2014.0499","volume":"25","author":"C Haller","year":"2015","unstructured":"Haller C, et al. One-staged aptamer-based isolation and application of endothelial progenitor cells in a porcine myocardial infarction model. Nucleic Acid Ther. 2015;25(1):20\u20136.","journal-title":"Nucleic Acid Ther"},{"issue":"25","key":"368_CR21","doi-asserted-by":"publisher","first-page":"3165","DOI":"10.1161\/CIRCULATIONAHA.106.687376","volume":"115","author":"DW Losordo","year":"2007","unstructured":"Losordo DW, et al. Intramyocardial transplantation of autologous CD34+ stem cells for intractable angina: a phase I\/IIa double-blind, randomized controlled trial. Circulation. 2007;115(25):3165\u201372.","journal-title":"Circulation"},{"issue":"4","key":"368_CR22","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1161\/CIRCRESAHA.111.245993","volume":"109","author":"DW Losordo","year":"2011","unstructured":"Losordo DW, et al. Intramyocardial, autologous CD34+ cell therapy for refractory angina. Circ Res. 2011;109(4):428\u201336.","journal-title":"Circ Res"},{"key":"368_CR23","doi-asserted-by":"crossref","unstructured":"Povsic TJ, et al. A phase 3, randomized, double-blinded, active-controlled, unblinded standard of care study assessing the efficacy and safety of intramyocardial autologous CD34+ cell administration in patients with refractory angina: design of the RENEW study. Am Heart J. 2013;165(6):854\u201361. e2.","DOI":"10.1016\/j.ahj.2013.03.003"},{"issue":"6","key":"368_CR24","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1161\/CIRCINTERVENTIONS.112.968321","volume":"5","author":"DW Losordo","year":"2012","unstructured":"Losordo DW, et al. A randomized, controlled pilot study of autologous CD34+ cell therapy for critical limb ischemia. Circ Cardiovasc Interv. 2012;5(6):821\u201330.","journal-title":"Circ Cardiovasc Interv"},{"issue":"8","key":"368_CR25","first-page":"14447","volume":"8","author":"SH Li","year":"2015","unstructured":"Li SH, et al. Exogenous hTERT gene transfected endothelial progenitor cells from bone marrow promoted angiogenesis in ischemic myocardium of rats. Int J Clin Exp Med. 2015;8(8):14447\u201353.","journal-title":"Int J Clin Exp Med"},{"issue":"6","key":"368_CR26","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.3892\/mmr.2012.1112","volume":"6","author":"Q She","year":"2012","unstructured":"She Q, et al. Angiogenesis in a rat model following myocardial infarction induced by hypoxic regulation of VEGF(1)(6)(5) gene-transfected EPCs. Mol Med Rep. 2012;6(6):1281\u20137.","journal-title":"Mol Med Rep"},{"key":"368_CR27","doi-asserted-by":"crossref","unstructured":"Long J, et al. The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization of rat hindlimb ischemia model. J Vasc Surg. 2013;58(3):756\u201365. e2.","DOI":"10.1016\/j.jvs.2012.11.096"},{"issue":"3","key":"368_CR28","doi-asserted-by":"publisher","first-page":"608","DOI":"10.1016\/j.jvs.2009.05.049","volume":"50","author":"JX Yu","year":"2009","unstructured":"Yu JX, et al. Combination of stromal-derived factor-1alpha and vascular endothelial growth factor gene-modified endothelial progenitor cells is more effective for ischemic neovascularization. J Vasc Surg. 2009;50(3):608\u201316.","journal-title":"J Vasc Surg"},{"key":"368_CR29","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.thromres.2016.03.012","volume":"141","author":"A Flex","year":"2016","unstructured":"Flex A, et al. Human cord blood endothelial progenitors promote post-ischemic angiogenesis in immunocompetent mouse model. Thromb Res. 2016;141:106\u201311.","journal-title":"Thromb Res."},{"issue":"6","key":"368_CR30","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1038\/sj.gt.3301142","volume":"7","author":"T Asahara","year":"2000","unstructured":"Asahara T, Kalka C, Isner JM. Stem cell therapy and gene transfer for regeneration. Gene Ther. 2000;7(6):451\u20137.","journal-title":"Gene Ther"},{"issue":"3","key":"368_CR31","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1097\/MOH.0000000000000140","volume":"22","author":"MC Yoder","year":"2015","unstructured":"Yoder MC. Differentiation of pluripotent stem cells into endothelial cells. Curr Opin Hematol. 2015;22(3):252\u20137.","journal-title":"Curr Opin Hematol"},{"issue":"1","key":"368_CR32","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1002\/stem.1267","volume":"31","author":"MP White","year":"2013","unstructured":"White MP, et al. Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells. Stem Cells. 2013;31(1):92\u2013103.","journal-title":"Stem Cells"},{"issue":"2","key":"368_CR33","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1007\/s12015-014-9582-4","volume":"11","author":"L Wang","year":"2015","unstructured":"Wang L, et al. Gene and microRNA profiling of human induced pluripotent stem cell-derived endothelial cells. Stem Cell Rev. 2015;11(2):219\u201327.","journal-title":"Stem Cell Rev"},{"issue":"4","key":"368_CR34","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1016\/j.biomaterials.2012.10.039","volume":"34","author":"SJ Park","year":"2013","unstructured":"Park SJ, et al. A comparison of human cord blood- and embryonic stem cell-derived endothelial progenitor cells in the treatment of chronic wounds. Biomaterials. 2013;34(4):995\u20131003.","journal-title":"Biomaterials"},{"issue":"10","key":"368_CR35","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1038\/nrd4738","volume":"14","author":"EA Kimbrel","year":"2015","unstructured":"Kimbrel EA, Lanza R. Current status of pluripotent stem cells: moving the first therapies to the clinic. Nat Rev Drug Discov. 2015;14(10):681\u201392.","journal-title":"Nat Rev Drug Discov"},{"issue":"5","key":"368_CR36","doi-asserted-by":"publisher","first-page":"370","DOI":"10.3109\/07853890.2015.1051579","volume":"47","author":"OE Simonson","year":"2015","unstructured":"Simonson OE, et al. The safety of human pluripotent stem cells in clinical treatment. Ann Med. 2015;47(5):370\u201380.","journal-title":"Ann Med"},{"issue":"34","key":"368_CR37","doi-asserted-by":"publisher","first-page":"13793","DOI":"10.1073\/pnas.1205526109","volume":"109","author":"A Margariti","year":"2012","unstructured":"Margariti A, et al. Direct reprogramming of fibroblasts into endothelial cells capable of angiogenesis and reendothelialization in tissue-engineered vessels. Proc Natl Acad Sci U S A. 2012;109(34):13793\u20138.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"6","key":"368_CR38","doi-asserted-by":"publisher","first-page":"1366","DOI":"10.1161\/ATVBAHA.112.301167","volume":"33","author":"J Li","year":"2013","unstructured":"Li J, et al. Conversion of human fibroblasts to functional endothelial cells by defined factors. Arterioscler Thromb Vasc Biol. 2013;33(6):1366\u201375.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"14","key":"368_CR39","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1161\/CIRCULATIONAHA.113.007727","volume":"130","author":"JK Han","year":"2014","unstructured":"Han JK, et al. Direct conversion of adult skin fibroblasts to endothelial cells by defined factors. Circulation. 2014;130(14):1168\u201378.","journal-title":"Circulation"},{"key":"368_CR40","unstructured":"Lee S, et al. Direct reprogramming of human dermal fibroblasts into endothelial cells using a single transcription factor. Circulation. 2014;130 Suppl 2:A18205."},{"issue":"1","key":"368_CR41","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1073\/pnas.1413234112","volume":"112","author":"R Morita","year":"2015","unstructured":"Morita R, et al. ETS transcription factor ETV2 directly converts human fibroblasts into functional endothelial cells. Proc Natl Acad Sci U S A. 2015;112(1):160\u20135.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"368_CR42","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1016\/j.cell.2012.09.032","volume":"151","author":"M Ginsberg","year":"2012","unstructured":"Ginsberg M, et al. Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFbeta suppression. Cell. 2012;151(3):559\u201375.","journal-title":"Cell"},{"issue":"2","key":"368_CR43","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.ydbio.2014.02.018","volume":"389","author":"X Shi","year":"2014","unstructured":"Shi X, et al. Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages. Dev Biol. 2014;389(2):208\u201318.","journal-title":"Dev Biol"},{"issue":"1","key":"368_CR44","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.ydbio.2014.06.019","volume":"393","author":"MO Schupp","year":"2014","unstructured":"Schupp MO, et al. Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development. Dev Biol. 2014;393(1):71\u201383.","journal-title":"Dev Biol"},{"issue":"1","key":"368_CR45","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1161\/ATVBAHA.115.306430","volume":"36","author":"C Park","year":"2016","unstructured":"Park C, et al. Injury-mediated vascular regeneration requires endothelial ER71\/ETV2. Arterioscler Thromb Vasc Biol. 2016;36(1):86\u201396.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"1","key":"368_CR46","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1089\/cell.2012.0037","volume":"15","author":"S Foja","year":"2013","unstructured":"Foja S, et al. Hypoxia supports reprogramming of mesenchymal stromal cells via induction of embryonic stem cell-specific microRNA-302 cluster and pluripotency-associated genes. Cell Reprogram. 2013;15(1):68\u201379.","journal-title":"Cell Reprogram"},{"issue":"2","key":"368_CR47","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1016\/j.bbrc.2011.11.111","volume":"417","author":"H Shimada","year":"2012","unstructured":"Shimada H, et al. Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia. Biochem Biophys Res Commun. 2012;417(2):659\u201364.","journal-title":"Biochem Biophys Res Commun"},{"issue":"3","key":"368_CR48","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.stem.2009.08.001","volume":"5","author":"Y Yoshida","year":"2009","unstructured":"Yoshida Y, et al. Hypoxia enhances the generation of induced pluripotent stem cells. Cell Stem Cell. 2009;5(3):237\u201341.","journal-title":"Cell Stem Cell"},{"issue":"10","key":"368_CR49","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1177\/0022034513502204","volume":"92","author":"K Iida","year":"2013","unstructured":"Iida K, et al. Hypoxia-enhanced derivation of iPSCs from human dental pulp cells. J Dent Res. 2013;92(10):905\u201310.","journal-title":"J Dent Res"},{"issue":"2","key":"368_CR50","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/S0923-1811(01)00157-8","volume":"28","author":"WK Nahm","year":"2002","unstructured":"Nahm WK, Zhou L, Falanga V. Sustained ability for fibroblast outgrowth from stored neonatal foreskin: a model for studying mechanisms of fibroblast outgrowth. J Dermatol Sci. 2002;28(2):152\u20138.","journal-title":"J Dermatol Sci"},{"issue":"5","key":"368_CR51","doi-asserted-by":"publisher","first-page":"654","DOI":"10.15252\/embr.201439939","volume":"16","author":"F Liu","year":"2015","unstructured":"Liu F, et al. Induction of hematopoietic and endothelial cell program orchestrated by ETS transcription factor ER71\/ETV2. EMBO Rep. 2015;16(5):654\u201369.","journal-title":"EMBO Rep"},{"issue":"17","key":"368_CR52","doi-asserted-by":"publisher","first-page":"2004","DOI":"10.1089\/scd.2013.0525","volume":"23","author":"AN Behrens","year":"2014","unstructured":"Behrens AN, et al. Sox7 is regulated by ETV2 during cardiovascular development. Stem Cells Dev. 2014;23(17):2004\u201313.","journal-title":"Stem Cells Dev"},{"key":"368_CR53","doi-asserted-by":"publisher","first-page":"4372","DOI":"10.1038\/ncomms5372","volume":"5","author":"I Elcheva","year":"2014","unstructured":"Elcheva I, et al. Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators. Nat Commun. 2014;5:4372.","journal-title":"Nat Commun."},{"issue":"1","key":"368_CR54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13619-014-0014-3","volume":"4","author":"AG Lindgren","year":"2015","unstructured":"Lindgren AG, Veldman MB, Lin S. ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells. Cell Regen (Lond). 2015;4(1):1.","journal-title":"Cell Regen (Lond)"},{"issue":"4","key":"368_CR55","doi-asserted-by":"publisher","first-page":"865","DOI":"10.1161\/ATVBAHA.114.304768","volume":"35","author":"MP Craig","year":"2015","unstructured":"Craig MP, et al. Etv2 and fli1b function together as key regulators of vasculogenesis and angiogenesis. Arterioscler Thromb Vasc Biol. 2015;35(4):865\u201376.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"4","key":"368_CR56","first-page":"283","volume":"30","author":"Z Zhang","year":"2014","unstructured":"Zhang Z, et al. The effects of conditioned medium from keloid fibroblasts under hypoxia on angiogenesis. Zhonghua Zheng Xing Wai Ke Za Zhi. 2014;30(4):283\u20138.","journal-title":"Zhonghua Zheng Xing Wai Ke Za Zhi"},{"issue":"7","key":"368_CR57","first-page":"1253","volume":"24","author":"J Jackson","year":"1997","unstructured":"Jackson J, et al. Expression of vascular endothelial growth factor in synovial fibroblasts is induced by hypoxia and interleukin 1beta. J Rheumatol. 1997;24(7):1253\u20139.","journal-title":"J Rheumatol"},{"issue":"3","key":"368_CR58","first-page":"374","volume":"71","author":"A Minchenko","year":"1994","unstructured":"Minchenko A, et al. Hypoxic stimulation of vascular endothelial growth factor expression in vitro and in vivo. Lab Invest. 1994;71(3):374\u20139.","journal-title":"Lab Invest"},{"issue":"10","key":"368_CR59","doi-asserted-by":"publisher","first-page":"2587","DOI":"10.1002\/art.10520","volume":"46","author":"C Hitchon","year":"2002","unstructured":"Hitchon C, et al. Hypoxia-induced production of stromal cell\u2013derived factor 1 (CXCL12) and vascular endothelial growth factor by synovial fibroblasts. Arthritis Rheum. 2002;46(10):2587\u201397.","journal-title":"Arthritis Rheum"},{"issue":"2","key":"368_CR60","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1006\/jsre.1999.5627","volume":"84","author":"DS Steinbrech","year":"1999","unstructured":"Steinbrech DS, et al. Fibroblast response to hypoxia: the relationship between angiogenesis and matrix regulation. J Surg Res. 1999;84(2):127\u201333.","journal-title":"J Surg Res"},{"issue":"5","key":"368_CR61","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1097\/00000637-199905000-00009","volume":"42","author":"DS Steinbrech","year":"1999","unstructured":"Steinbrech DS, et al. Hypoxia upregulates VEGF production in keloid fibroblasts. Ann Plast Surg. 1999;42(5):514\u201320.","journal-title":"Ann Plast Surg"},{"issue":"4","key":"368_CR62","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1111\/j.1524-475X.2009.00499.x","volume":"17","author":"EY Lee","year":"2009","unstructured":"Lee EY, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen. 2009;17(4):540\u20137.","journal-title":"Wound Repair Regen"},{"issue":"10","key":"368_CR63","doi-asserted-by":"publisher","first-page":"1292","DOI":"10.1161\/01.CIR.0000121425.42966.F1","volume":"109","author":"J Rehman","year":"2004","unstructured":"Rehman J, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004;109(10):1292\u20138.","journal-title":"Circulation"},{"key":"368_CR64","doi-asserted-by":"crossref","unstructured":"Van Pham P, et al. Hypoxia condition promoted the adipose derived stem cell proliferation via VEGF production. Biomed Res Ther. 2016;3(1):476-82.","DOI":"10.7603\/s40730-016-0004-x"}],"container-title":["Stem Cell Research &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-016-0368-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13287-016-0368-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-016-0368-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-016-0368-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T06:16:32Z","timestamp":1637043392000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-016-0368-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,4]]},"references-count":64,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["368"],"URL":"https:\/\/doi.org\/10.1186\/s13287-016-0368-2","relation":{},"ISSN":["1757-6512"],"issn-type":[{"type":"electronic","value":"1757-6512"}],"subject":[],"published":{"date-parts":[[2016,8,4]]},"assertion":[{"value":"27 April 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 July 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 August 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"104"}}