{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:05:44Z","timestamp":1789416344564,"version":"build-2803163510"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2015R1A2A2A01006490"],"award-info":[{"award-number":["2015R1A2A2A01006490"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s13287-017-0725-9","type":"journal-article","created":{"date-parts":[[2017,12,6]],"date-time":"2017-12-06T01:14:27Z","timestamp":1512522867000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models"],"prefix":"10.1186","volume":"8","author":[{"given":"Jin-Sun","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EunJi","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonggu","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Lhi","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9227-8303","authenticated-orcid":false,"given":"Jung Sun","family":"Heo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,12,6]]},"reference":[{"key":"725_CR1","doi-asserted-by":"crossref","unstructured":"Du J, Li M. Functions of Periostin in dental tissues and its role in periodontal tissues' regeneration. Cell Mol Life Sci. 2017;74(23):4279\u20134286.","DOI":"10.1007\/s00018-017-2645-3"},{"issue":"1","key":"725_CR2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.dental.2016.10.003","volume":"33","author":"SB Qasim","year":"2017","unstructured":"Qasim SB, Najeeb S, Delaine-Smith RM, Rawlinson A, Ur RI. Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration. Dent Mater. 2017;33(1):71\u201383.","journal-title":"Dent Mater"},{"issue":"1","key":"725_CR3","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.bbrc.2017.01.065","volume":"484","author":"K Sakaguchi","year":"2017","unstructured":"Sakaguchi K, Katagiri W, Osugi M, Kawai T, Sugimura-Wakayama Y, Hibi H. Periodontal tissue regeneration using the cytokine cocktail mimicking secretomes in the conditioned media from human mesenchymal stem cells. Biochem Biophys Res Commun. 2017;484(1):100\u20136.","journal-title":"Biochem Biophys Res Commun"},{"issue":"2","key":"725_CR4","doi-asserted-by":"crossref","first-page":"123","DOI":"10.15171\/joddd.2017.022","volume":"11","author":"V Kl","year":"2017","unstructured":"Kl V, Ryana H, Dalvi PJ. Autologous periodontal stem cell assistance in periodontal regeneration technique (SAI-PRT) in the treatment of periodontal intrabony defects: a case report with one-year follow-up. J Dent Res Dent Clin Dent Prospects. 2017;11(2):123\u20136.","journal-title":"J Dent Res Dent Clin Dent Prospects"},{"issue":"6","key":"725_CR5","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1080\/03008207.2016.1191478","volume":"57","author":"H Cho","year":"2016","unstructured":"Cho H, Tarafder S, Fogge M, Kao K, Lee CH. Periodontal ligament stem\/progenitor cells with protein-releasing scaffolds for cementum formation and integration on dentin surface. Connect Tissue Res. 2016;57(6):488\u201395.","journal-title":"Connect Tissue Res"},{"key":"725_CR6","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1159\/000366381","volume":"34","author":"JS Lee","year":"2014","unstructured":"Lee JS, Yi JK, An SY, Heo JS. Increased osteogenic differentiation of periodontal ligament stem cells on polydopamine film occurs via activation of integrin and PI3K signaling pathways. Cell Physiol Biochem. 2014;34:1824\u201334.","journal-title":"Cell Physiol Biochem"},{"issue":"6","key":"725_CR7","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/jre.12356","volume":"51","author":"U Ripamonti","year":"2016","unstructured":"Ripamonti U. Redefining the induction of periodontal tissue regeneration in primates by the osteogenic proteins of the transforming growth factor-\u03b2 supergene family. J Periodontal Res. 2016;51(6):699\u2013715.","journal-title":"J Periodontal Res"},{"issue":"1","key":"725_CR8","doi-asserted-by":"crossref","first-page":"6373","DOI":"10.1038\/s41598-017-06548-7","volume":"7","author":"L Nie","year":"2017","unstructured":"Nie L, Yang X, Duan L, Huang E, Pengfei Z, Luo W, Zhang Y, Zeng X, Qiu Y, Cai T, Li C. The healing of alveolar bone defects with novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds. Sci Rep. 2017;7(1):6373.","journal-title":"Sci Rep"},{"issue":"10","key":"725_CR9","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1097\/BRS.0b013e31827eca09","volume":"38","author":"Y Tan","year":"2013","unstructured":"Tan Y, Montgomery SR, Aghdasi BG, Inoue H, Kaner T, Tian H, Terrell R, Zhang X, Wang JC, Daubs MD. The effect of corticosteroid administration on soft-tissue inflammation associated with rhBMP-2 use in a rodent model of inflammation. Spine. 2013;38(10):806\u201313.","journal-title":"Spine"},{"issue":"6","key":"725_CR10","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.spinee.2010.05.016","volume":"11","author":"SD Glassman","year":"2011","unstructured":"Glassman SD, Gum JL, Crawford 3rd CH, Shields CB, Carreon LY. Complications with recombinant human bone morphogenetic protein-2 in posterolateral spine fusion associated with a dural tear. Spine J. 2011;11(6):522\u20136.","journal-title":"Spine J"},{"key":"725_CR11","first-page":"561","volume":"2015","author":"IK Kwon","year":"1853","unstructured":"Kwon IK, Lee SC, Hwang YS, Heo JS. Mitochondrial function contributes to oxysterol-induced osteogenic differentiation in mouse embryonic stem cells. Biochim Biophys Acta. 1853;2015:561\u201372.","journal-title":"Biochim Biophys Acta"},{"key":"725_CR12","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.redox.2017.07.014","volume":"13","author":"F Luchetti","year":"2017","unstructured":"Luchetti F, Crinelli R, Cesarini E, Canonico B, Guidi L, Zerbinati C, Di Sario G, Zamai L, Magnani M, Papa S, Iuliano L. Endothelial cells, endoplasmic reticulum stress and oxysterols. Redox Biol. 2017;13:581\u20137.","journal-title":"Redox Biol"},{"key":"725_CR13","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1002\/jor.20437","volume":"25","author":"TL Aghaloo","year":"2007","unstructured":"Aghaloo TL, Amantea CM, Cowan CM, Richardson JA, Wu BM, Parhami F, et al. Oxysterols enhance osteoblast differentiation in vitro and bone healing in vivo. J Orthop Res. 2007;25:1488\u201397.","journal-title":"J Orthop Res"},{"key":"725_CR14","doi-asserted-by":"crossref","unstructured":"Buser Z, Drapeau S, Stappenbeck F, Pereira RC, Parhami F, Wang JC. Effect of Oxy133, an osteogenic oxysterol, on new bone formation in rat two-level posterolateral fusion model. Eur Spine J. 2017;26(11):2763\u20132772.","DOI":"10.1007\/s00586-017-5149-9"},{"key":"725_CR15","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1002\/jcb.23082","volume":"112","author":"JS Johnson","year":"2011","unstructured":"Johnson JS, Meliton V, Kim WK, Lee KB, Wang JC, Nguyen K, et al. Novel oxysterols have pro-osteogenic and anti-adipogenic effects in vitro and induce spinal fusion in vivo. J Cell Biochem. 2011;112:1673\u201384.","journal-title":"J Cell Biochem"},{"key":"725_CR16","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1359\/jbmr.091024","volume":"25","author":"WK Kim","year":"2010","unstructured":"Kim WK, Meliton V, Tetradis S, Weinmaster G, Hahn TJ, Carlson M, et al. Osteogenic oxysterol, 20(S)-hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells. J Bone Miner Res. 2010;25:782\u201395.","journal-title":"J Bone Miner Res"},{"key":"725_CR17","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/jcb.21840","volume":"105","author":"CM Amantea","year":"2008","unstructured":"Amantea CM, Kim WK, Meliton V, Tetradis S, Parhami F. Oxysterol-induced osteogenic differentiation of marrow stromal cells is regulated by Dkk-1 inhibitable and PI3-kinase mediated signaling. J Cell Biochem. 2008;105:424\u201336.","journal-title":"J Cell Biochem"},{"key":"725_CR18","doi-asserted-by":"crossref","first-page":"8959","DOI":"10.1074\/jbc.M611741200","volume":"282","author":"JR Dwyer","year":"2007","unstructured":"Dwyer JR, Sever N, Carlson M, Nelson SF, Beachy PA, Parhami F. Oxysterols are novel activators of the hedgehog signaling pathway in pluripotent mesenchymal cells. J Biol Chem. 2007;282:8959\u201368.","journal-title":"J Biol Chem"},{"key":"725_CR19","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1359\/jbmr.040115","volume":"19","author":"HT Kha","year":"2004","unstructured":"Kha HT, Basseri B, Shouhed D, Richardson J, Tetradis S, Hahn TJ, et al. Oxysterols regulate differentiation of mesenchymal stem cells: pro-bone and anti-fat. J Bone Miner Res. 2004;19:830\u201340.","journal-title":"J Bone Miner Res"},{"key":"725_CR20","first-page":"146","volume":"364","author":"BM Seo","year":"2004","unstructured":"Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004;364:146\u201355.","journal-title":"Lancet"},{"key":"725_CR21","doi-asserted-by":"crossref","first-page":"e67504","DOI":"10.1371\/journal.pone.0067504","volume":"28","author":"SY Kim","year":"2013","unstructured":"Kim SY, Lee JY, Park YD, Kang KL, Lee JC, Heo JS. Hesperetin alleviates the inhibitory effects of high glucose on the osteoblastic differentiation of periodontal ligament stem cells. PLoS One. 2013;28:e67504.","journal-title":"PLoS One"},{"key":"725_CR22","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248\u201354.","journal-title":"Anal Biochem"},{"key":"725_CR23","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s10059-011-0107-6","volume":"32","author":"LJ Qiao","year":"2011","unstructured":"Qiao LJ, Kang KL, Heo JS. Simvastatin promotes osteogenic differentiation of mouse embryonic stem cells via canonical Wnt\/\u03b2-catenin signaling. Mol Cells. 2011;32:437\u201344.","journal-title":"Mol Cells"},{"key":"725_CR24","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.steroids.2016.12.003","volume":"118","author":"E\u00d6 Viktorsson","year":"2017","unstructured":"Viktorsson E\u00d6, Gabrielsen M, Kumarachandran N, Sylte I, Rongved P, \u00c5strand OA, Kase ET. Regulation of liver X receptor target genes by 22-functionalized oxysterols. Synthesis, in silico and in vitro evaluations. Steroids. 2017;118:119\u201327.","journal-title":"Steroids"},{"issue":"8","key":"725_CR25","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/j.bbagrm.2017.05.007","volume":"1860","author":"M Kanaki","year":"2017","unstructured":"Kanaki M, Tiniakou I, Thymiakou E, Kardassis D. Physical and functional interactions between nuclear receptor LXR\u03b1 and the forkhead box transcription factor FOXA2 regulate the response of the human lipoprotein lipase gene to oxysterols in hepatic cells. Biochim Biophys Acta. 2017;1860(8):848\u201360.","journal-title":"Biochim Biophys Acta"},{"issue":"8","key":"725_CR26","doi-asserted-by":"crossref","first-page":"2810","DOI":"10.1210\/en.2013-2143","volume":"155","author":"T Sugizaki","year":"2014","unstructured":"Sugizaki T, Watanabe M, Horai Y, Kaneko-Iwasaki N, Arita E, Miyazaki T, Morimoto K, Honda A, Irie J, Itoh H. Decreased liver X receptor (LXR) activity in liver of obese male mice. Endocrinology. 2014;155(8):2810\u20139.","journal-title":"Endocrinology"},{"key":"725_CR27","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1210\/me.2008-0453","volume":"23","author":"WK Kim","year":"2009","unstructured":"Kim WK, Meliton V, Park KW, Hong C, Tontonoz P, Niewiadomski P, et al. Negative regulation of Hedgehog signaling by liver X receptors. Mol Endocrinol. 2009;23:1532\u201343.","journal-title":"Mol Endocrinol"},{"key":"725_CR28","doi-asserted-by":"crossref","first-page":"4691","DOI":"10.1210\/en.2011-1298","volume":"152","author":"ER Nelson","year":"2011","unstructured":"Nelson ER, DuSell CD, Wang X, Howe MK, Evans G, Michalek RD, et al. The oxysterol, 27-hydroxycholesterol, links cholesterol metabolism to bone homeostasis through its actions on the estrogen and liver X receptors. Endocrinology. 2011;152:4691\u2013705.","journal-title":"Endocrinology"},{"key":"725_CR29","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.bone.2010.08.018","volume":"48","author":"J Prawitt","year":"2011","unstructured":"Prawitt J, Beil FT, Marshall RP, Bartelt A, Ruether W, Heeren J, et al. Short-term activation of liver X receptors inhibits osteoblasts but long-term activation does not have an impact on murine bone in vivo. Bone. 2011;48:339\u201346.","journal-title":"Bone"},{"key":"725_CR30","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.bone.2017.07.017","volume":"103","author":"X Liu","year":"2017","unstructured":"Liu X, McKenzie JA, Maschhoff CW, Gardner MJ, Silva MJ. Exogenous hedgehog antagonist delays but does not prevent fracture healing in young mice. Bone. 2017;103:241\u201351.","journal-title":"Bone"},{"key":"725_CR31","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.spinee.2014.11.014","volume":"15","author":"TP Scott","year":"2015","unstructured":"Scott TP, Phan KH, Tian H, Suzuki A, Montgomery SR, Johnson JS, et al. Comparison of a novel oxysterol molecule and rhBMP2 fusion rates in a rabbit posterolateral lumbar spine model. Spine J. 2015;15:733\u201342.","journal-title":"Spine J"},{"key":"725_CR32","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1002\/term.1799","volume":"10","author":"A Hokugo","year":"2016","unstructured":"Hokugo A, Sorice S, Parhami F, Yalom A, Li A, Zuk P, et al. A novel oxysterol promotes bone regeneration in rabbit cranial bone defects. J Tissue Eng Regen Med. 2016;10:591\u20139.","journal-title":"J Tissue Eng Regen Med"},{"key":"725_CR33","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1227\/01.NEU.0000369517.21018.F2","volume":"66","author":"MP Garrett","year":"2010","unstructured":"Garrett MP, Kakarla UK, Porter RW, Sonntag VK. Formation of painful seroma and edema after the use of recombinant human bone morphogenetic protein-2 in posterolateral lumbar spine fusions. Neurosurgery. 2010;66:1044\u20139.","journal-title":"Neurosurgery"},{"key":"725_CR34","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4103\/2152-7806.76147","volume":"2","author":"NE Epstein","year":"2011","unstructured":"Epstein NE. Pros, cons, and costs of INFUSE in spinal surgery. Surg Neurol Int. 2011;2:10.","journal-title":"Surg Neurol Int"}],"container-title":["Stem Cell Research & Therapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-017-0725-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T12:51:43Z","timestamp":1719665503000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-017-0725-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["725"],"URL":"https:\/\/doi.org\/10.1186\/s13287-017-0725-9","relation":{},"ISSN":["1757-6512"],"issn-type":[{"value":"1757-6512","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12]]},"article-number":"276"}}