{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:43:26Z","timestamp":1790315006434,"version":"4.1.0"},"reference-count":64,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T00:00:00Z","timestamp":1552867200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000341","name":"Arthritis Research UK","doi-asserted-by":"publisher","award":["19425"],"award-info":[{"award-number":["19425"]}],"id":[{"id":"10.13039\/501100000341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000341","name":"Arthritis Research UK","doi-asserted-by":"publisher","award":["20253"],"award-info":[{"award-number":["20253"]}],"id":[{"id":"10.13039\/501100000341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000341","name":"Arthritis Research UK","doi-asserted-by":"publisher","award":["21156"],"award-info":[{"award-number":["21156"]}],"id":[{"id":"10.13039\/501100000341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MC_PC_16049"],"award-info":[{"award-number":["MC_PC_16049"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s13287-019-1202-4","type":"journal-article","created":{"date-parts":[[2019,3,18]],"date-time":"2019-03-18T08:03:16Z","timestamp":1552896196000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells"],"prefix":"10.1186","volume":"10","author":[{"given":"Claire","family":"Mennan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Hulme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,3,18]]},"reference":[{"key":"1202_CR1","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1097\/00007890-196803000-00009","volume":"6","author":"AJ Friedenstein","year":"1968","unstructured":"Friedenstein AJ, Petrakova KV, Kurolesova AI, Frolova GP. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968;6:230\u201347.","journal-title":"Transplantation."},{"key":"1202_CR2","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1002\/jor.1100090504","volume":"9","author":"AI Caplan","year":"1991","unstructured":"Caplan AI. Mesenchymal stem cells. J Orthop Res. 1991;9:641\u201350.","journal-title":"J Orthop Res"},{"key":"1202_CR3","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1126\/science.284.5411.143","volume":"284","author":"MF Pittenger","year":"1999","unstructured":"Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143\u20137.","journal-title":"Science."},{"key":"1202_CR4","doi-asserted-by":"crossref","first-page":"24295","DOI":"10.1038\/srep24295","volume":"6","author":"J Garcia","year":"2016","unstructured":"Garcia J, Wright K, Roberts S, Kuiper JH, Mangham C, Richardson J, et al. Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: the influence of tissue source and inflammatory stimulus. Sci Rep. 2016;6:24295.","journal-title":"Sci Rep"},{"key":"1202_CR5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/916136","volume":"2013","author":"C Mennan","year":"2013","unstructured":"Mennan C, Wright K, Bhattacharjee A, Balain B, Richardson J, Roberts S. Isolation and characterisation of mesenchymal stem cells from different regions of the human umbilical cord. Biomed Res Int. 2013;2013:1\u20138.","journal-title":"Biomed Res Int"},{"key":"1202_CR6","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1080\/14653240600855905","volume":"8","author":"M Dominici","year":"2006","unstructured":"Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8:315\u20137.","journal-title":"Cytotherapy."},{"key":"1202_CR7","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.jcyt.2013.02.006","volume":"15","author":"P Bourin","year":"2013","unstructured":"Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal\/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013;15:641\u20138.","journal-title":"Cytotherapy"},{"key":"1202_CR8","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1111\/j.1365-2249.2007.03422.x","volume":"149","author":"JM Ryan","year":"2007","unstructured":"Ryan JM, Barry F, Murphy JM, Mahon BP. Interferon-\u03b3 does not break, but promotes the immunosuppressive capacity of adult human mesenchymal stem cells. Clin Exp Immunol. 2007;149:353\u201363.","journal-title":"Clin Exp Immunol"},{"key":"1202_CR9","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/j.jcyt.2013.02.010","volume":"15","author":"M Krampera","year":"2013","unstructured":"Krampera M, Galipeau J, Shi Y, Tarte K, Sensebe L. Immunological characterization of multipotent mesenchymal stromal cells--the International Society for Cellular Therapy (ISCT) working proposal. Cytotherapy. 2013;15:1054\u201361.","journal-title":"Cytotherapy"},{"key":"1202_CR10","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1634\/stemcells.2005-0008","volume":"24","author":"M Krampera","year":"2006","unstructured":"Krampera M, Cosmi L, Angeli R, Pasini A, Liotta F, Andreini A, et al. Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Stem Cells. 2006;24:386\u201398.","journal-title":"Stem Cells"},{"key":"1202_CR11","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/mt.2011.189","volume":"20","author":"M Fran\u00e7ois","year":"2012","unstructured":"Fran\u00e7ois M, Romieu-Mourez R, Li M, Galipeau J. Human MSC suppression correlates with cytokine induction of indoleamine 2,3-dioxygenase and bystander M2 macrophage differentiation. Mol Ther. 2012;20:187\u201395.","journal-title":"Mol Ther"},{"key":"1202_CR12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/ncomms15040","volume":"8","author":"AJ Roelofs","year":"2017","unstructured":"Roelofs AJ, Zupan J, Riemen AHK, Kania K, Ansboro S, White N, et al. Joint morphogenetic cells in the adult synovium. Nat Commun. 2017;8:1\u201314.","journal-title":"Nat Commun"},{"key":"1202_CR13","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1002\/stem.118","volume":"27","author":"G Ren","year":"2009","unstructured":"Ren G, Su J, Zhang L, Zhao X, Ling W, L\u2019Huillie A, et al. Species variation in the mechanisms of mesenchymal stem cell-mediated immunosuppression. Stem Cells. 2009;27:1954\u201362.","journal-title":"Stem Cells"},{"key":"1202_CR14","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1016\/j.arthro.2013.09.074","volume":"29","author":"KL Wong","year":"2013","unstructured":"Wong KL, Lee KBL, Tai BC, Law P, Lee EH, Hui JHP. Injectable cultured bone marrow\u2013derived mesenchymal stem cells in Varus knees with cartilage defects undergoing high tibial osteotomy: a prospective, randomized controlled clinical trial with 2 years\u2019 follow-up. Arthroscopy. 2013;29:2020\u20138.","journal-title":"Arthroscopy"},{"key":"1202_CR15","doi-asserted-by":"crossref","first-page":"4209","DOI":"10.3892\/mmr.2016.5745","volume":"14","author":"C Chen","year":"2016","unstructured":"Chen C, Qu Z, Yin X, Shang C, Ao Q, Gu Y, et al. Efficacy of umbilical cord-derived mesenchymal stem cell-based therapy for osteonecrosis of the femoral head: a three-year follow-up study. Mol Med Rep. 2016;14:4209\u201315.","journal-title":"Mol Med Rep"},{"key":"1202_CR16","first-page":"4209","volume":"14","author":"JM Lamo-Espinosa","year":"2016","unstructured":"Lamo-Espinosa JM, Mora G, Blanco JF, Granero-Molt\u00f3 F, Nu\u00f1ez-C\u00f3rdoba JM, S\u00e1nchez-Echenique C, et al. Intra-articular injection of two different doses of autologous bone marrow mesenchymal stem cells versus hyaluronic acid in the treatment of knee osteoarthritis: multicenter randomized controlled clinical trial (phase I\/II). J Transl Med. 2016;14:4209\u201315.","journal-title":"J Transl Med"},{"key":"1202_CR17","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/s13018-017-0689-6","volume":"12","author":"M Al-Najar","year":"2017","unstructured":"Al-Najar M, Khalil H, Al-Ajlouni J, Al-Antary E, Hamdan M, Rahmeh R, et al. Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I\/II study. J Orthop Surg Res. 2017;12:190.","journal-title":"J Orthop Surg Res"},{"key":"1202_CR18","doi-asserted-by":"crossref","first-page":"493","DOI":"10.2217\/rme-2017-0032","volume":"12","author":"JB Richardson","year":"2017","unstructured":"Richardson JB, Wright KT, Wales J, Kuiper JH, McCarthy HS, Gallacher P, et al. Efficacy and safety of autologous cell therapies for knee cartilage defects (autologous stem cells, chondrocytes or the two): randomized controlled trial design. Regen Med. 2017;12:493\u2013501.","journal-title":"Regen Med"},{"key":"1202_CR19","doi-asserted-by":"crossref","first-page":"595","DOI":"10.3727\/000000004783983747","volume":"13","author":"S Wakitani","year":"2004","unstructured":"Wakitani S, Mitsuoka T, Nakamura N, Toritsuka Y, Nakamura Y, Horibe S. Autologous bone marrow stromal cell transplantation for repair of full-thickness articular cartilage defects in human patellae: two case reports. Cell Transplant. 2004;13:595\u2013600.","journal-title":"Cell Transplant"},{"key":"1202_CR20","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1186\/1479-5876-11-143","volume":"11","author":"PK Gupta","year":"2013","unstructured":"Gupta PK, Chullikana A, Parakh R, Desai S, Das A, Gottipamula S, et al. A double blind randomized placebo controlled phase I\/II study assessing the safety and efficacy of allogeneic bone marrow derived mesenchymal stem cell in critical limb ischemia. J Transl Med. 2013;11:143.","journal-title":"J Transl Med"},{"key":"1202_CR21","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1097\/TP.0000000000000678","volume":"99","author":"A Vega","year":"2015","unstructured":"Vega A, Mart\u00edn-Ferrero MA, Del Canto F, Alberca M, Garc\u00eda V, Munar A, et al. Treatment of knee osteoarthritis with allogeneic bone marrow mesenchymal stem cells: a randomized controlled trial. Transplantation. 2015;99:1681\u201390.","journal-title":"Transplantation."},{"key":"1202_CR22","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1136\/annrheumdis-2017-211073","volume":"76","author":"D Deng","year":"2017","unstructured":"Deng D, Zhang P, Guo Y, Lim TO. A randomised double-blind, placebo-controlled trial of allogeneic umbilical cord-derived mesenchymal stem cell for lupus nephritis. Ann Rheum Dis. 2017;76:1436\u20139.","journal-title":"Ann Rheum Dis"},{"key":"1202_CR23","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jcyt.2015.10.001","volume":"18","author":"I Oliver-vila","year":"2016","unstructured":"Oliver-vila I, Coca MI, Grau-vorster M, Pujals-fonts N, Caminal M, Casamayor-genesc\u00e0 A, et al. Evaluation of a cell-banking strategy for the production of clinical grade mesenchymal stromal cells from Wharton\u2019s jelly. Cytotherapy. 2016;18:25\u201335.","journal-title":"Cytotherapy."},{"key":"1202_CR24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/scrt477","volume":"5","author":"P Swamynathan","year":"2014","unstructured":"Swamynathan P, Venugopal P, Kannan S, Thej C, Kolkundar U. Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton\u2019 s jelly derived mesenchymal stem cells ? A comparative study. Stem Cell Res Ther. 2014;5:1\u201317.","journal-title":"Stem Cell Res Ther"},{"key":"1202_CR25","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.scr.2010.08.005","volume":"5","author":"U Nekanti","year":"2010","unstructured":"Nekanti U, Mohanty L, Venugopal P. Optimization and scale-up of Wharton\u2019 s jelly-derived mesenchymal stem cells for clinical applications. Stem Cell Res. 2010;5:244\u201354.","journal-title":"Stem Cell Res"},{"key":"1202_CR26","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1016\/j.jcyt.2013.05.024","volume":"15","author":"M Jones","year":"2013","unstructured":"Jones M, Varella-Garcia M, Skokan M, Bryce S, Schowinsky J, Peters R, et al. Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System. Cytotherapy. 2013;15:1323\u201339.","journal-title":"Cytotherapy."},{"key":"1202_CR27","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.bbrc.2012.11.001","volume":"430","author":"P Nold","year":"2013","unstructured":"Nold P, Brendel C, Neubauer A, Bein G, Hackstein H. Good manufacturing practice-compliant animal-free expansion of human bone marrow derived mesenchymal stroma cells in a closed hollow-fiber-based bioreactor. Biochem Biophys Res Commun. 2013;430:325\u201330.","journal-title":"Biochem Biophys Res Commun"},{"key":"1202_CR28","first-page":"1","volume":"0","author":"M Haack-S\u00f8rensen","year":"2018","unstructured":"Haack-S\u00f8rensen M, Juhl M, Follin B, Harary S\u00f8ndergaard R, Kirchhoff M, Kastrup J, et al. Development of large-scale manufacturing of adipose-derived stromal cells for clinical applications using bioreactors and human platelet lysate. Scand J Clin Lab Invest. 2018;0:1\u20138.","journal-title":"Scand J Clin Lab Invest"},{"key":"1202_CR29","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.3727\/096368912X657990","volume":"22","author":"MT Rojewski","year":"2013","unstructured":"Rojewski MT, Fekete N, Baila S, Nguyen K, F\u00fcrst D, Antwiler D, et al. GMP-compliant isolation and expansion of bone marrow-derived MSCs in the closed, automated device quantum cell expansion system. Cell Transplant. 2013;22:1981\u20132000.","journal-title":"Cell Transplant"},{"key":"1202_CR30","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.jcyt.2014.01.417","volume":"16","author":"PJ Hanley","year":"2014","unstructured":"Hanley PJ, Mei Z, Durett AG, da Graca C-HM, Klis M, Li W, et al. Efficient manufacturing of therapeutic mesenchymal stromal cells with the use of the quantum cell expansion system. Cytotherapy. 2014;16:1048\u201358.","journal-title":"Cytotherapy"},{"key":"1202_CR31","doi-asserted-by":"publisher","unstructured":"Lechanteur C, Baila S, Janssens ME, Giet O, Briquet A, et al. Large-scale clinical expansion of mesenchymal stem cells in the GMP-compliant, closed automated Quantum\u00ae cell expansion system: comparison with expansion in traditional T-flasks. J Stem Cell Res Ther. 2014;4. \n                    https:\/\/doi.org\/10.4172\/2157-7633.1000222\n                    \n                  .","DOI":"10.4172\/2157-7633.1000222"},{"key":"1202_CR32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12967-016-1080-9","volume":"14","author":"M Haack-S\u00f8rensen","year":"2016","unstructured":"Haack-S\u00f8rensen M, Follin B, Juhl M, Brorsen SK, S\u00f8ndergaard RH, Kastrup J, et al. Culture expansion of adipose derived stromal cells. A closed automated quantum cell expansion system compared with manual flask-based culture. J Transl Med. 2016;14:1\u201311.","journal-title":"J Transl Med"},{"key":"1202_CR33","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/j.jcyt.2016.05.013","volume":"18","author":"T Lambrechts","year":"2016","unstructured":"Lambrechts T, Papantoniou I, Rice B, Schrooten J, Luyten FP, Aerts JM. Large-scale progenitor cell expansion for multiple donors in a monitored hollow fibre bioreactor. Cytotherapy. 2016;18:1219\u201333.","journal-title":"Cytotherapy"},{"key":"1202_CR34","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1002\/sctm.17-0040","volume":"6","author":"J Kastrup","year":"2017","unstructured":"Kastrup J, Haack-Sorensen M, Juhl M, Sondergaard R, Follin B, Lund L, et al. Cryopreserved off-the-shelf allogeneic adipose- derived stromal cells for therapy in patients with ischemic heart disease and heart failure \u2014 a safety study. Stem Cells Transl Med. 2017;6:1963\u201371.","journal-title":"Stem Cells Transl Med"},{"key":"1202_CR35","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/j.jcyt.2017.07.004","volume":"19","author":"I Kozanoglu","year":"2017","unstructured":"Kozanoglu I, Maytalman E, Gereklioglu C, Yeral M, Buyukkurt N, Aytan P, et al. Quantum cell expansion system: safe and rapid expansion. Cytotherapy. 2017;19:1246\u20137.","journal-title":"Cytotherapy"},{"key":"1202_CR36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15761\/JSCRM.1000123","volume":"2","author":"C Pirrone","year":"2017","unstructured":"Pirrone C, Gobbetti A, Caprara C, Bernardini G, Gornati R, Soldati G. Chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor. J Stem Cell Res Med. 2017;2:1\u201310.","journal-title":"J Stem Cell Res Med"},{"key":"1202_CR37","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1089\/scd.2008.0291","volume":"18","author":"M Petrini","year":"2009","unstructured":"Petrini M, Pacini S, Trombi L, Fazzi R, Montali M, Ikehara S, et al. Identification and purification of mesodermal progenitor cells from human adult bone marrow. Stem Cells Dev. 2009;18:857\u201366.","journal-title":"Stem Cells Dev"},{"key":"1202_CR38","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1097\/01.shk.0000235087.45798.93","volume":"26","author":"PR Crisostomo","year":"2006","unstructured":"Crisostomo PR, Wang M, Wairiuko GM, Morrell ED, Terrell AM, Seshadri P, et al. High passage number of stem cells adversely affects stem cell activation and myocardial protection. Shock. 2006;26:575\u201380.","journal-title":"Shock."},{"key":"1202_CR39","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1002\/2211-5463.12104","volume":"6","author":"C Mennan","year":"2016","unstructured":"Mennan C, Brown S, McCarthy H, Mavrogonatou E, Kletsas D, Garcia J, et al. Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton\u2019s jelly and bone marrow. FEBS Open Bio. 2016;6:1054\u201366.","journal-title":"FEBS Open Bio"},{"key":"1202_CR40","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1159\/000058758","volume":"74","author":"F Stelter","year":"2000","unstructured":"Stelter F. Structure\/function relationships of CD14. Chem Immunol. 2000;74:25\u201341.","journal-title":"Chem Immunol"},{"key":"1202_CR41","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/j.1365-2249.1997.507-ce1404.x","volume":"110","author":"J Rugtveit","year":"1997","unstructured":"Rugtveit J, Bakka A, Brandtzaeg P. Differential distribution of B7.1 (CD80) and B7.2 (CD86) costimulatory molecules on mucosal macrophage subsets in human inflammatory bowel disease (IBD). Clin Exp Immunol. 1997;110:104\u201313.","journal-title":"Clin Exp Immunol"},{"key":"1202_CR42","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.imbio.2005.05.010","volume":"210","author":"BO Fabriek","year":"2005","unstructured":"Fabriek BO, Dijkstra CD, van den Berg TK. The macrophage scavenger receptor CD163. Immunobiology. 2005;210:153\u201360.","journal-title":"Immunobiology."},{"key":"1202_CR43","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.imbio.2008.11.004","volume":"214","author":"U Gazi","year":"2009","unstructured":"Gazi U, Martinez-Pomares L. Influence of the mannose receptor in host immune responses. Immunobiology. 2009;214:554\u201361.","journal-title":"Immunobiology"},{"key":"1202_CR44","doi-asserted-by":"crossref","first-page":"655","DOI":"10.3727\/096368910X536473","volume":"20","author":"T Deuse","year":"2011","unstructured":"Deuse T, Stubbendorff M, Tang-Quan K, Phillips N, Kay MA, Eiermann T, et al. Immunogenicity and immunomodulatory properties of umbilical cord lining mesenchymal stem cells. Cell Transplant. 2011;20:655\u201367.","journal-title":"Cell Transplant"},{"key":"1202_CR45","volume-title":"Multilineage potential of adult human mesenchymal stem cells","author":"MF Pittenger","year":"1999","unstructured":"Pittenger MF, Mackay AM, Beck SC, Jaiswal RK. Multilineage potential of adult human mesenchymal stem cells; 1999."},{"key":"1202_CR46","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.0.CO;2-I","volume":"64","author":"N Jaiswal","year":"1997","unstructured":"Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem. 1997;64:295\u2013312.","journal-title":"J Cell Biochem"},{"key":"1202_CR47","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(\u2212Delta Delta C(T)) method. Methods. 2001;25:402\u20138.","journal-title":"Methods."},{"key":"1202_CR48","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.omtm.2018.05.006","volume":"10","author":"R Tirughana","year":"2018","unstructured":"Tirughana R, Metz MZ, Li Z, Hall C, Hsu D, Beltzer J, et al. GMP production and scale-up of adherent neural stem cells with a quantum cell expansion system. Mol Ther Methods Clin Dev. 2018;10:48\u201356.","journal-title":"Mol Ther Methods Clin Dev"},{"key":"1202_CR49","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1471-2121-7-14","volume":"7","author":"M Bonab","year":"2006","unstructured":"Bonab M, Alimoghaddam K, Talebian F, Ghaffari S, Ghavamzadeh A, Nikbin B. Aging of mesenchymal stem cell in vitro. BMC Cell Biol. 2006;7:14.","journal-title":"BMC Cell Biol"},{"key":"1202_CR50","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1093\/cvr\/cvs202","volume":"96","author":"J Muller-Ehmsen","year":"2012","unstructured":"Muller-Ehmsen J. The problem is obvious, the solution is not: numbers do matter in cardiac cell therapy! Cardiovasc Res. 2012;96:208\u20139.","journal-title":"Cardiovasc Res"},{"key":"1202_CR51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/732742","volume":"2013","author":"TJ Kean","year":"2013","unstructured":"Kean TJ, Lin P, Caplan AI, Dennis JE. MSCs: delivery routes and engraftment, cell-targeting strategies, and immune modulation. Stem Cells Int. 2013;2013:1\u201313.","journal-title":"Stem Cells Int"},{"key":"1202_CR52","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1089\/107632702760240535","volume":"8","author":"PJ Brugge ter","year":"2002","unstructured":"ter Brugge PJ, Torensma R, De Ruijter JE, Figdor CG, Jansen JA. Modulation of integrin expression on rat bone marrow cells by substrates with different surface characteristics. Tissue Eng. 2002;8:615\u201326.","journal-title":"Tissue Eng"},{"key":"1202_CR53","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1083\/jcb.142.2.587","volume":"142","author":"A Pozzi","year":"1998","unstructured":"Pozzi A, Wary KK, Giancotti FG, Gardner HA. Integrin alpha1beta1 mediates a unique collagen-dependent proliferation pathway in vivo. J Cell Biol. 1998;142:587\u201394.","journal-title":"J Cell Biol"},{"key":"1202_CR54","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/S0002-9440(10)64751-7","volume":"156","author":"G Hou","year":"2000","unstructured":"Hou G, Mulholland D, Gronska MA, Bendeck MP. Type VIII collagen stimulates smooth muscle cell migration and matrix metalloproteinase synthesis after arterial injury. Am J Pathol. 2000;156:467\u201376.","journal-title":"Am J Pathol"},{"key":"1202_CR55","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0002-9440(10)64363-5","volume":"160","author":"DR Senger","year":"2002","unstructured":"Senger DR, Perruzzi CA, Streit M, Koteliansky VE, de Fougerolles AR, Detmar M. The alpha(1)beta(1) and alpha(2)beta(1) integrins provide critical support for vascular endothelial growth factor signaling, endothelial cell migration, and tumor angiogenesis. Am J Pathol. 2002;160:195\u2013204.","journal-title":"Am J Pathol"},{"key":"1202_CR56","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1016\/S0002-9440(10)61124-8","volume":"160","author":"E Ekholm","year":"2002","unstructured":"Ekholm E, Hankenson KD, Uusitalo H, Hiltunen A, Gardner H, Heino J, et al. Diminished callus size and cartilage synthesis in alpha 1 beta 1 integrin-deficient mice during bone fracture healing. Am J Pathol. 2002;160:1779\u201385.","journal-title":"Am J Pathol"},{"key":"1202_CR57","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1111\/j.1365-2567.2005.02224.x","volume":"116","author":"S Rond Le","year":"2005","unstructured":"Le Rond S, Gonzalez A, Gonzalez ASL, Carosella ED, Rouas-Freiss N. Indoleamine 2,3 dioxygenase and human leucocyte antigen-G inhibit the T-cell alloproliferative response through two independent pathways. Immunology. 2005;116:297\u2013307.","journal-title":"Immunology."},{"key":"1202_CR58","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1002\/cbin.10023","volume":"37","author":"K Hao","year":"2013","unstructured":"Hao K, Zhou Q, Chen W, Jia W, Zheng J, Kang J, et al. Possible role of the \u2018IDO-AhR axis\u2019 in maternal-foetal tolerance. Cell Biol Int. 2013;37:105\u20138.","journal-title":"Cell Biol Int"},{"key":"1202_CR59","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.jcyt.2017.12.013","volume":"20","author":"S Savelli","year":"2018","unstructured":"Savelli S, Trombi L, D\u2019Alessandro D, Moscato S, Pacini S, Giannotti S, et al. Pooled human serum: a new culture supplement for bioreactor-based cell therapies. Preliminary results. Cytotherapy. 2018;20:556\u201363.","journal-title":"Cytotherapy"},{"key":"1202_CR60","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1038\/nri2448","volume":"8","author":"DM Mosser","year":"2008","unstructured":"Mosser DM, Edwards JP. Exploring the full spectrum of macrophage activation. Nat Rev Immunol. 2008;8:958\u201369.","journal-title":"Nat Rev Immunol"},{"key":"1202_CR61","doi-asserted-by":"crossref","first-page":"13","DOI":"10.12703\/P6-13","volume":"6","author":"FO Martinez","year":"2014","unstructured":"Martinez FO, Gordon S. The M1 and M2 paradigm of macrophage activation: time for reassessment. F1000Prime Rep. 2014;6:13.","journal-title":"F1000Prime Rep"},{"key":"1202_CR62","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/path.4133","volume":"229","author":"A Mantovani","year":"2013","unstructured":"Mantovani A, Biswas SK, Galdiero MR, Sica A, Locati M. Macrophage plasticity and polarization in tissue repair and remodelling. J Pathol. 2013;229:176\u201385.","journal-title":"J Pathol"},{"key":"1202_CR63","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1089\/ten.teb.2014.0098","volume":"21","author":"N Fahy","year":"2015","unstructured":"Fahy N, Farrell E, Ritter T, Ryan AE, Murphy JM. Immune modulation to improve tissue engineering outcomes for cartilage repair in the osteoarthritic joint. Tissue Eng Part B Rev. 2015;21:55\u201366.","journal-title":"Tissue Eng Part B Rev"},{"key":"1202_CR64","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.1111\/trf.14805","volume":"58","author":"AL Russell","year":"2018","unstructured":"Russell AL, Lefavor RC, Zubair AC. Characterization and cost-benefit analysis of automated bioreactor-expanded mesenchymal stem cells for clinical applications. Transfusion. 2018;58:2374\u201382.","journal-title":"Transfusion."}],"container-title":["Stem Cell Research &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-019-1202-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13287-019-1202-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-019-1202-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T20:30:22Z","timestamp":1584390622000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-019-1202-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,18]]},"references-count":64,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["1202"],"URL":"https:\/\/doi.org\/10.1186\/s13287-019-1202-4","relation":{},"ISSN":["1757-6512"],"issn-type":[{"value":"1757-6512","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,18]]},"assertion":[{"value":"9 January 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 February 2019","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 March 2019","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 March 2019","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Bone marrow was received from a commercial source (Lonza), with informed consent from healthy male donors. Umbilical cords were collected with informed maternal consent. Favourable ethical approval was given by the Greater Manchester East National Research Ethics Service (10\/H10130\/62).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Publisher\u2019s Note"}}],"article-number":"99"}}