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Herein, our study was performed to evaluate the effect of exosomal miR-4532 secreted by AML cells on hematopoiesis of hematopoietic stem cells.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Firstly, differentially expressed miRs related to AML were identified using microarray analysis. Subsequently, AML cell lines were collected, and CD34<jats:sup>+<\/jats:sup> HSCs were isolated from healthy pregnant women. Then, miR-4532 expression was measured in AML cells and AML cell-derived exosomes and CD34<jats:sup>+<\/jats:sup> HSCs, together with evaluation of the targeting relationship between miR-4532 and LDOC1. Then, AML cells were treated with miR-4532 inhibitor, and exosomes were separated from AML cells and co-cultured with CD34<jats:sup>+<\/jats:sup> HSCs. Gain- and loss-function approaches were employed in CD34<jats:sup>+<\/jats:sup> HSCs. Colony-forming units (CFU) and expression of dickkopf-1 (DKK1), a hematopoietic inhibiting factor associated with pathogenesis of AML, were determined in CD34<jats:sup>+<\/jats:sup> HSCs, as well as the extents of JAK2 and STAT3 phosphorylation and LDOC1 expression.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>miR-4532 was found to be upregulated in AML cells and AML cell-derived exosomes, while being downregulated in CD34<jats:sup>+<\/jats:sup> HSCs. In addition, exosomes released by AML cells targeted CD34<jats:sup>+<\/jats:sup> HSCs to decrease the expression of CFU and increase the expression of DKK1. miR-4532 was delivered into CD34<jats:sup>+<\/jats:sup> HSCs to target LDOC1 via AML cell-released exosomes. AML cell-derived exosomes containing miR-4532 inhibitor increased CFU but reduced DKK1 in CD34<jats:sup>+<\/jats:sup> HSCs. Inhibition of miR-4532 or JAK2, or ectopic expression of LDOC1 upregulated CFU and downregulated DKK1 expression as well as the extents of JAK2 and STAT3 phosphorylation in CD34<jats:sup>+<\/jats:sup> HSCs.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p>In conclusion, AML cell-derived exosomes carrying miR-4532 repress normal HSC hematopoiesis via activation of the LDOC1-dependent STAT3 signaling pathway.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13287-019-1475-7","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T14:02:48Z","timestamp":1576504968000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway"],"prefix":"10.1186","volume":"10","author":[{"given":"Chen","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,16]]},"reference":[{"issue":"4","key":"1475_CR1","first-page":"633","volume":"23","author":"HD Klepin","year":"2015","unstructured":"Klepin HD. 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