{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T09:00:56Z","timestamp":1781946056353,"version":"3.54.5"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100007046","name":"Wuhan University","doi-asserted-by":"publisher","award":["cxpy2017034"],"award-info":[{"award-number":["cxpy2017034"]}],"id":[{"id":"10.13039\/501100007046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Accidentally removed parathyroid glands are still challenging in neck surgery, leading to hypoparathyroidism characterized with abnormally low levels of parathyroid hormone. Parathyroid auto-transplantation is usually applied in compensation. To improve the efficiency of parathyroid transplantation, we introduced a method by co-transplanting with adipose-derived cells, including stromal vascular fractions (SVFs) and adipose-derived stem cells (ADSCs), and investigated the underlying molecular mechanisms involved in parathyroid transplantation survival.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Rat and human parathyroid tissues were transplanted into nude mice as parathyroid transplantation model to examine the effects of SVFs and ADSCs on grafts angiogenesis and survival rates, including blood vessel assembly and parathyroid hormone levels. Several angiogenic factors, such as vascular endothelial growth factor (VEGF)-A and fibroblast growth factor (FGF) 2, were assessed in parathyroid grafts. The effects of hypoxia were investigated on ADSCs. The modulatory roles of the eyes absent homolog 1 (EYA1), which is vital in parathyroid development, was also investigated on angiogenic factor production and secretion by ADSCs. All experimental data were statistically processed. Student\u2019s <jats:italic>t<\/jats:italic> test was used to assess significant differences between 2 groups. For multiple comparisons with additional interventions, two-way ANOVA followed by Tukey\u2019s post hoc test was performed. <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05 was considered as significant.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>SVFs improve rat parathyroid transplantation survival and blood vessel assembly, as well as FGF2 and VEGF-A expression levels in parathyroid transplantation mice. Functional human parathyroid grafts have higher microvessel density and increased VEGF-A expression. The supernatant of ADSCs induced tubule formation and migration of human endothelial cells in vitro. Hypoxia had no effect on proliferation and apoptosis of human ADSCs but induced higher angiogenic factor levels of VEGF-A and FGF2, modulated by EYA1, which was confirmed by parathyroid glands transplantation in mice.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Adipose-derived cells, including ADSCs and SVFs, improve parathyroid transplantation survival via promoting angiogenesis through EYA1-regulating angiogenetic factors in vitro and in vivo. Our studies proved an effective method to improve the parathyroid autotransplantation, which is promising for clinical patients with hypoparathyroidism when parathyroid glands were accidentally injured, removed, or devascularized.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13287-020-01733-4","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T07:03:29Z","timestamp":1590476609000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model"],"prefix":"10.1186","volume":"11","author":[{"given":"Qiuxia","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deguang","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianing","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangbo","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9296-7080","authenticated-orcid":false,"given":"Gaosong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,26]]},"reference":[{"issue":"Suppl 1","key":"1733_CR1","doi-asserted-by":"publisher","first-page":"83","DOI":"10.4158\/EP10377.RA","volume":"17","author":"JM Moffett","year":"2011","unstructured":"Moffett JM, Suliburk J. Parathyroid autotransplantation. Endocr Pract. 2011;17(Suppl 1):83\u20139.","journal-title":"Endocr Pract"},{"issue":"3","key":"1733_CR2","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1017\/S0022215106003501","volume":"121","author":"C Page","year":"2007","unstructured":"Page C, Strunski V. Parathyroid risk in total thyroidectomy for bilateral, benign, multinodular goitre: report of 351 surgical cases. J Laryngol Otol. 2007;121(3):237\u201341.","journal-title":"J Laryngol Otol"},{"issue":"9","key":"1733_CR3","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1089\/thy.2012.0011","volume":"22","author":"D Giordano","year":"2012","unstructured":"Giordano D, Valcavi R, Thompson GB, Pedroni C, Renna L, Gradoni P, et al. Complications of central neck dissection in patients with papillary thyroid carcinoma: results of a study on 1087 patients and review of the literature. Thyroid. 2012;22(9):911\u20137.","journal-title":"Thyroid"},{"issue":"2","key":"1733_CR4","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1007\/BF03401343","volume":"13","author":"ZC Etheridge","year":"2014","unstructured":"Etheridge ZC, Schofield C, Prinsloo PJ, Sturrock ND. Hypocalcaemia following thyroidectomy unresponsive to oral therapy. Hormones (Athens). 2014;13(2):286\u20139.","journal-title":"Hormones (Athens)"},{"issue":"4","key":"1733_CR5","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.endonu.2012.11.002","volume":"60","author":"A Larrad Jimenez","year":"2013","unstructured":"Larrad Jimenez A, Hernandez Hernandez JR. Parathyroid transplantation. Endocrinol Nutr. 2013;60(4):161\u20133.","journal-title":"Endocrinol Nutr"},{"issue":"6","key":"1733_CR6","doi-asserted-by":"publisher","first-page":"2583","DOI":"10.1210\/jc.2004-0535","volume":"89","author":"GA Bray","year":"2004","unstructured":"Bray GA. Medical consequences of obesity. J Clin Endocrinol Metab. 2004;89(6):2583\u20139.","journal-title":"J Clin Endocrinol Metab"},{"issue":"6","key":"1733_CR7","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/j.jcyt.2013.02.006","volume":"15","author":"P Bourin","year":"2013","unstructured":"Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal\/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013;15(6):641\u20138.","journal-title":"Cytotherapy."},{"issue":"12","key":"1733_CR8","doi-asserted-by":"publisher","first-page":"4279","DOI":"10.1091\/mbc.e02-02-0105","volume":"13","author":"PA Zuk","year":"2002","unstructured":"Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002;13(12):4279\u201395.","journal-title":"Mol Biol Cell"},{"issue":"2","key":"1733_CR9","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.ymeth.2008.03.006","volume":"45","author":"BA Bunnell","year":"2008","unstructured":"Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C. Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 2008;45(2):115\u201320.","journal-title":"Methods."},{"issue":"3","key":"1733_CR10","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1111\/wrr.12542","volume":"25","author":"E Brett","year":"2017","unstructured":"Brett E, Zielins ER, Chin M, Januszyk M, Blackshear CP, Findlay M, et al. Isolation of CD248-expressing stromal vascular fraction for targeted improvement of wound healing. Wound Repair Regen. 2017;25(3):414\u201322.","journal-title":"Wound Repair Regen"},{"issue":"1","key":"1733_CR11","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1186\/s13287-016-0377-1","volume":"7","author":"J Chen","year":"2016","unstructured":"Chen J, Gu Z, Wu M, Yang Y, Zhang J, Ou J, et al. C-reactive protein can upregulate VEGF expression to promote ADSC-induced angiogenesis by activating HIF-1alpha via CD64\/PI3k\/Akt and MAPK\/ERK signaling pathways. Stem Cell Res Ther. 2016;7(1):114.","journal-title":"Stem Cell Res Ther"},{"issue":"4","key":"1733_CR12","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1097\/01.TP.0000151633.92173.75","volume":"79","author":"S Strieth","year":"2005","unstructured":"Strieth S, von Johnston V, Eichhorn ME, Enders G, Krasnici S, Thein E, et al. A new animal model to assess angiogenesis and endocrine function of parathyroid heterografts in vivo. Transplantation. 2005;79(4):392\u2013400.","journal-title":"Transplantation."},{"issue":"1","key":"1733_CR13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115034","volume":"10","author":"JR Choi","year":"2015","unstructured":"Choi JR, Pingguan-Murphy B, Wan Abas WA, Yong KW, Poon CT, Noor Azmi MA, et al. In situ normoxia enhances survival and proliferation rate of human adipose tissue-derived stromal cells without increasing the risk of tumourigenesis. PLoS One. 2015;10(1):e0115034.","journal-title":"PLoS One"},{"issue":"21\u201322","key":"1733_CR14","doi-asserted-by":"publisher","first-page":"2330","DOI":"10.1089\/ten.tea.2012.0750","volume":"19","author":"ML Skiles","year":"2013","unstructured":"Skiles ML, Sahai S, Rucker L, Blanchette JO. Use of culture geometry to control hypoxia-induced vascular endothelial growth factor secretion from adipose-derived stem cells: optimizing a cell-based approach to drive vascular growth. Tissue Eng Part A. 2013;19(21\u201322):2330\u20138.","journal-title":"Tissue Eng Part A"},{"issue":"14","key":"1733_CR15","doi-asserted-by":"publisher","first-page":"2587","DOI":"10.1007\/s00018-017-2484-2","volume":"74","author":"JR Choi","year":"2017","unstructured":"Choi JR, Yong KW, Wan Safwani WKZ. Effect of hypoxia on human adipose-derived mesenchymal stem cells and its potential clinical applications. Cell Mol Life Sci. 2017;74(14):2587\u2013600.","journal-title":"Cell Mol Life Sci"},{"issue":"6398","key":"1733_CR16","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1038\/359843a0","volume":"359","author":"D Shweiki","year":"1992","unstructured":"Shweiki D, Itin A, Soffer D, Keshet E. Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature. 1992;359(6398):843\u20135.","journal-title":"Nature."},{"issue":"1","key":"1733_CR17","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1002\/cbin.10170","volume":"38","author":"JH Kim","year":"2014","unstructured":"Kim JH, Kim SH, Song SY, Kim WS, Song SU, Yi T, et al. Hypoxia induces adipocyte differentiation of adipose-derived stem cells by triggering reactive oxygen species generation. Cell Biol Int. 2014;38(1):32\u201340.","journal-title":"Cell Biol Int"},{"issue":"1","key":"1733_CR18","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/j.bbrc.2018.02.076","volume":"497","author":"YD Han","year":"2018","unstructured":"Han YD, Bai Y, Yan XL, Ren J, Zeng Q, Li XD, et al. Co-transplantation of exosomes derived from hypoxia-preconditioned adipose mesenchymal stem cells promotes neovascularization and graft survival in fat grafting. Biochem Biophys Res Commun. 2018;497(1):305\u201312.","journal-title":"Biochem Biophys Res Commun"},{"issue":"2","key":"1733_CR19","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1016\/j.bbrc.2014.04.096","volume":"448","author":"JR Choi","year":"2014","unstructured":"Choi JR, Pingguan-Murphy B, Wan Abas WA, Noor Azmi MA, Omar SZ, Chua KH, et al. Impact of low oxygen tension on stemness, proliferation and differentiation potential of human adipose-derived stem cells. Biochem Biophys Res Commun. 2014;448(2):218\u201324.","journal-title":"Biochem Biophys Res Commun"},{"issue":"6964","key":"1733_CR20","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1038\/nature02083","volume":"426","author":"X Li","year":"2003","unstructured":"Li X, Oghi KA, Zhang J, Krones A, Bush KT, Glass CK, et al. Eya protein phosphatase activity regulates Six1-Dach-Eya transcriptional effects in mammalian organogenesis. Nature. 2003;426(6964):247\u201354.","journal-title":"Nature."},{"key":"1733_CR21","first-page":"12","volume":"2","author":"Y Liu","year":"2015","unstructured":"Liu Y, Kong D, Wu H, Yuan X, Xu H, Zhang C, et al. Interplay of retinal determination gene network with TGF-beta signaling pathway in epithelial-mesenchymal transition. Stem Cell Investig. 2015;2:12.","journal-title":"Stem Cell Investig"},{"issue":"30","key":"1733_CR22","doi-asserted-by":"publisher","first-page":"12384","DOI":"10.1073\/pnas.0906930108","volume":"108","author":"A Watanabe","year":"2011","unstructured":"Watanabe A, Ogiwara H, Ehata S, Mukasa A, Ishikawa S, Maeda D, et al. Homozygously deleted gene DACH1 regulates tumor-initiating activity of glioma cells. Proc Natl Acad Sci U S A. 2011;108(30):12384\u20139.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"1733_CR23","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.ydbio.2013.07.019","volume":"382","author":"K Lu","year":"2013","unstructured":"Lu K, Reddy R, Berika M, Warburton D, El-Hashash AH. Abrogation of Eya1\/Six1 disrupts the saccular phase of lung morphogenesis and causes remodeling. Dev Biol. 2013;382(1):110\u201323.","journal-title":"Dev Biol"},{"issue":"3","key":"1733_CR24","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1007\/s10456-014-9420-y","volume":"17","author":"NS Vasudev","year":"2014","unstructured":"Vasudev NS, Reynolds AR. Anti-angiogenic therapy for cancer: current progress, unresolved questions and future directions. Angiogenesis. 2014;17(3):471\u201394.","journal-title":"Angiogenesis."},{"issue":"11","key":"1733_CR25","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1007\/s00018-012-1144-9","volume":"70","author":"E Tadjuidje","year":"2013","unstructured":"Tadjuidje E, Hegde RS. The eyes absent proteins in development and disease. Cell Mol Life Sci. 2013;70(11):1897\u2013913.","journal-title":"Cell Mol Life Sci"},{"key":"1733_CR26","doi-asserted-by":"publisher","first-page":"51","DOI":"10.3389\/fnmol.2011.00051","volume":"4","author":"J Karar","year":"2011","unstructured":"Karar J, Maity A. PI3K\/AKT\/mTOR pathway in angiogenesis. Front Mol Neurosci. 2011;4:51.","journal-title":"Front Mol Neurosci"},{"issue":"4","key":"1733_CR27","first-page":"586","volume":"6","author":"X Wang","year":"2013","unstructured":"Wang X, Chen X, Fang J, Yang C. Overexpression of both VEGF-A and VEGF-C in gastric cancer correlates with prognosis, and silencing of both is effective to inhibit cancer growth. Int J Clin Exp Pathol. 2013;6(4):586\u201397.","journal-title":"Int J Clin Exp Pathol"},{"issue":"1","key":"1733_CR28","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1002\/jcp.21584","volume":"218","author":"F Dayan","year":"2009","unstructured":"Dayan F, Bilton RL, Laferriere J, Trottier E, Roux D, Pouyssegur J, et al. Activation of HIF-1alpha in exponentially growing cells via hypoxic stimulation is independent of the Akt\/mTOR pathway. J Cell Physiol. 2009;218(1):167\u201374.","journal-title":"J Cell Physiol"},{"issue":"23","key":"1733_CR29","doi-asserted-by":"publisher","first-page":"4653","DOI":"10.1182\/blood-2012-04-421040","volume":"120","author":"G Christoffersson","year":"2012","unstructured":"Christoffersson G, Vagesjo E, Vandooren J, Liden M, Massena S, Reinert RB, et al. VEGF-A recruits a proangiogenic MMP-9-delivering neutrophil subset that induces angiogenesis in transplanted hypoxic tissue. Blood. 2012;120(23):4653\u201362.","journal-title":"Blood."},{"issue":"20","key":"1733_CR30","doi-asserted-by":"publisher","first-page":"10490","DOI":"10.1074\/jbc.M115.683201","volume":"291","author":"H Nishida-Fukuda","year":"2016","unstructured":"Nishida-Fukuda H, Araki R, Shudou M, Okazaki H, Tomono Y, Nakayama H, et al. Ectodomain shedding of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) is induced by vascular endothelial growth factor a (VEGF-A). J Biol Chem. 2016;291(20):10490\u2013500.","journal-title":"J Biol Chem"},{"issue":"1","key":"1733_CR31","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.yexcr.2018.02.028","volume":"367","author":"S Cai","year":"2018","unstructured":"Cai S, Cheng X, Liu Y, Lin Z, Zeng W, Yang C, et al. EYA1 promotes tumor angiogenesis by activating the PI3K pathway in colorectal cancer. Exp Cell Res. 2018;367(1):37\u201346.","journal-title":"Exp Cell Res"},{"issue":"12","key":"1733_CR32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084582","volume":"8","author":"RN Pandey","year":"2013","unstructured":"Pandey RN, Wang TS, Tadjuidje E, McDonald MG, Rettie AE, Hegde RS. Structure-activity relationships of benzbromarone metabolites and derivatives as EYA inhibitory anti-angiogenic agents. PLoS One. 2013;8(12):e84582.","journal-title":"PLoS One"},{"key":"1733_CR33","doi-asserted-by":"publisher","first-page":"41781","DOI":"10.1038\/srep41781","volume":"7","author":"G Yang","year":"2017","unstructured":"Yang G, Xiao Z, Ren X, Long H, Ma K, Qian H, et al. Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels. Sci Rep. 2017;7:41781.","journal-title":"Sci Rep"},{"issue":"4","key":"1733_CR34","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.112.000877","volume":"1","author":"Y Shimizu","year":"2012","unstructured":"Shimizu Y, Shibata R, Shintani S, Ishii M, Murohara T. Therapeutic lymphangiogenesis with implantation of adipose-derived regenerative cells. J Am Heart Assoc. 2012;1(4):e000877.","journal-title":"J Am Heart Assoc"},{"key":"1733_CR35","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.exger.2015.01.044","volume":"63","author":"AL Aird","year":"2015","unstructured":"Aird AL, Nevitt CD, Christian K, Williams SK, Hoying JB, LeBlanc AJ. Adipose-derived stromal vascular fraction cells isolated from old animals exhibit reduced capacity to support the formation of microvascular networks. Exp Gerontol. 2015;63:18\u201326.","journal-title":"Exp Gerontol"},{"issue":"1","key":"1733_CR36","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/S0039-6109(16)41742-1","volume":"59","author":"SA Wells Jr","year":"1979","unstructured":"Wells SA Jr, Ross AJ 3rd, Dale JK, Gray RS. Transplantation of the parathyroid glands: current status. Surg Clin North Am. 1979;59(1):167\u201377.","journal-title":"Surg Clin North Am"},{"issue":"4","key":"1733_CR37","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/j.surg.2009.10.010","volume":"147","author":"S Borot","year":"2010","unstructured":"Borot S, Lapierre V, Carnaille B, Goudet P, Penfornis A. Results of cryopreserved parathyroid autografts: a retrospective multicenter study. Surgery. 2010;147(4):529\u201335.","journal-title":"Surgery."},{"issue":"6","key":"1733_CR38","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1016\/j.surg.2005.09.029","volume":"138","author":"MS Cohen","year":"2005","unstructured":"Cohen MS, Dilley WG, Wells SA Jr, Moley JF, Doherty GM, Sicard GA, et al. Long-term functionality of cryopreserved parathyroid autografts: a 13-year prospective analysis. Surgery. 2005;138(6):1033\u201340 discussion 40-1.","journal-title":"Surgery."},{"issue":"5","key":"1733_CR39","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1007\/s00268-007-9437-0","volume":"32","author":"MA Guerrero","year":"2008","unstructured":"Guerrero MA, Evans DB, Lee JE, Bao R, Bereket A, Gantela S, et al. Viability of cryopreserved parathyroid tissue: when is continued storage versus disposal indicated? World J Surg. 2008;32(5):836\u20139.","journal-title":"World J Surg"},{"issue":"3\u20134","key":"1733_CR40","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1089\/ten.tea.2015.0277","volume":"22","author":"R Kosaraju","year":"2016","unstructured":"Kosaraju R, Rennert RC, Maan ZN, Duscher D, Barrera J, Whittam AJ, et al. Adipose-derived stem cell-seeded hydrogels increase endogenous progenitor cell recruitment and neovascularization in wounds. Tissue Eng Part A. 2016;22(3\u20134):295\u2013305.","journal-title":"Tissue Eng Part A."},{"issue":"5","key":"1733_CR41","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1084\/jem.176.5.1375","volume":"176","author":"LF Brown","year":"1992","unstructured":"Brown LF, Yeo KT, Berse B, Yeo TK, Senger DR, Dvorak HF, et al. Expression of vascular permeability factor (vascular endothelial growth factor) by epidermal keratinocytes during wound healing. J Exp Med. 1992;176(5):1375\u20139.","journal-title":"J Exp Med"},{"issue":"16","key":"1733_CR42","doi-asserted-by":"crossref","first-page":"10931","DOI":"10.1016\/S0021-9258(19)49853-0","volume":"267","author":"J Folkman","year":"1992","unstructured":"Folkman J, Shing Y. Angiogenesis. J Biol Chem. 1992;267(16):10931\u20134.","journal-title":"J Biol Chem"},{"issue":"3","key":"1733_CR43","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1016\/j.bbrc.2007.09.058","volume":"363","author":"S Sadat","year":"2007","unstructured":"Sadat S, Gehmert S, Song YH, Yen Y, Bai X, Gaiser S, et al. The cardioprotective effect of mesenchymal stem cells is mediated by IGF-I and VEGF. Biochem Biophys Res Commun. 2007;363(3):674\u20139.","journal-title":"Biochem Biophys Res Commun"},{"issue":"1","key":"1733_CR44","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/j.jss.2009.09.057","volume":"165","author":"H Abdollahi","year":"2011","unstructured":"Abdollahi H, Harris LJ, Zhang P, McIlhenny S, Srinivas V, Tulenko T, et al. The role of hypoxia in stem cell differentiation and therapeutics. J Surg Res. 2011;165(1):112\u20137.","journal-title":"J Surg Res"},{"issue":"12","key":"1733_CR45","doi-asserted-by":"publisher","first-page":"1499","DOI":"10.1517\/14712590903307362","volume":"9","author":"HM Chung","year":"2009","unstructured":"Chung HM, Won CH, Sung JH. Responses of adipose-derived stem cells during hypoxia: enhanced skin-regenerative potential. Expert Opin Biol Ther. 2009;9(12):1499\u2013508.","journal-title":"Expert Opin Biol Ther"},{"issue":"5","key":"1733_CR46","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1002\/jcb.20904","volume":"99","author":"R Izadpanah","year":"2006","unstructured":"Izadpanah R, Trygg C, Patel B, Kriedt C, Dufour J, Gimble JM, et al. Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem. 2006;99(5):1285\u201397.","journal-title":"J Cell Biochem"},{"issue":"1","key":"1733_CR47","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1002\/jcp.20463","volume":"206","author":"F Guilak","year":"2006","unstructured":"Guilak F, Lott KE, Awad HA, Cao Q, Hicok KC, Fermor B, et al. Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. J Cell Physiol. 2006;206(1):229\u201337.","journal-title":"J Cell Physiol"},{"key":"1733_CR48","doi-asserted-by":"publisher","first-page":"1450","DOI":"10.3389\/fonc.2019.01450","volume":"9","author":"D Kong","year":"2019","unstructured":"Kong D, Li A, Liu Y, Cui Q, Wang K, Zhang D, et al. SIX1 activates STAT3 signaling to promote the proliferation of thyroid carcinoma via EYA1. Front Oncol. 2019;9:1450.","journal-title":"Front Oncol"},{"issue":"5","key":"1733_CR49","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1242\/dev.077255","volume":"139","author":"AM Anderson","year":"2012","unstructured":"Anderson AM, Weasner BM, Weasner BP, Kumar JP. Dual transcriptional activities of SIX proteins define their roles in normal and ectopic eye development. Development. 2012;139(5):991\u20131000.","journal-title":"Development."},{"issue":"19","key":"1733_CR50","doi-asserted-by":"publisher","first-page":"2527","DOI":"10.1038\/onc.2014.179","volume":"34","author":"Y Sun","year":"2015","unstructured":"Sun Y, Kaneko S, Li XK, Li X. The PI3K\/Akt signal hyperactivates Eya1 via the SUMOylation pathway. Oncogene. 2015;34(19):2527\u201337.","journal-title":"Oncogene."}],"container-title":["Stem Cell Research &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-020-01733-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13287-020-01733-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-020-01733-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T23:25:23Z","timestamp":1621985123000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-020-01733-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,26]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["1733"],"URL":"https:\/\/doi.org\/10.1186\/s13287-020-01733-4","relation":{},"ISSN":["1757-6512"],"issn-type":[{"value":"1757-6512","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,26]]},"assertion":[{"value":"25 February 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 April 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 May 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 May 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study received ethical clearance from the Ethics Committee for Animal Experiments of Tongji Medical College and the Research Board from Wuhan University. Human tissues were obtained from 2 patients with their written, informed consent. All experiments were carried out in accordance with Declaration of Helsinki and other recognized standards.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"200"}}