{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T20:54:52Z","timestamp":1778187292980,"version":"3.51.4"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T00:00:00Z","timestamp":1609977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T00:00:00Z","timestamp":1609977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1083857"],"award-info":[{"award-number":["APP1083857"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1130013"],"award-info":[{"award-number":["APP1130013"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Bone marrow stromal cells (BMSC) show promise in cartilage repair, and sheep are the most common large animal pre-clinical model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this study was to characterise ovine BMSC (oBMSC) in vitro, and to evaluate the capacity of chondrogenic micro-pellets manufactured from oBMSC or ovine articular chondrocytes (oACh) to repair osteochondral defects in sheep.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>\n                      oBMSC were characterised for surface marker expression using flow cytometry and evaluated for tri-lineage differentiation\u00a0capacity. oBMSC micro-pellets were manufactured in a microwell platform, and chondrogenesis was compared at 2%, 5%, and 20% O\n                      <jats:sub>2<\/jats:sub>\n                      . The capacity of cartilage micro-pellets manufactured from oBMSC or oACh to repair osteochondral defects in adult sheep was evaluated in an 8-week pilot study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Expanded oBMSC were positive for CD44 and CD146 and negative for CD45. The common adipogenic induction ingredient, 3-Isobutyl-1-methylxanthine (IBMX), was toxic to oBMSC, but adipogenesis could be restored by excluding IBMX\u00a0from the medium. BMSC chondrogenesis was optimal in a 2% O\n                      <jats:sub>2<\/jats:sub>\n                      atmosphere. Micro-pellets formed from oBMSC or oACh appeared morphologically similar, but hypertrophic genes were elevated in oBMSC micro-pellets. While oACh micro-pellets formed cartilage-like repair tissue in sheep, oBMSC micro-pellets did not.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The sensitivity of oBMSC, compared\u00a0to human BMSC, to IBMX in standard adipogenic assays highlights species-associated differences. Micro-pellets manufactured from oACh were more effective than micro-pellets manufactured from\u00a0oBMSC\u00a0in\u00a0the repair of osteochondral defects in sheep. While oBMSC can be driven to form cartilage-like tissue in vitro, the effective use of these cells in cartilage repair will depend on the successful mitigation of hypertrophy and tissue integration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13287-020-02045-3","type":"journal-article","created":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T09:03:57Z","timestamp":1610010237000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Characterisation of ovine bone marrow-derived stromal cells (oBMSC) and evaluation of chondrogenically induced micro-pellets for cartilage tissue repair in vivo"],"prefix":"10.1186","volume":"12","author":[{"given":"K.","family":"Futrega","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Music","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. G.","family":"Robey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Gronthos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Crawford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Saifzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. J.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5876-4757","authenticated-orcid":false,"given":"M. R.","family":"Doran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,1,7]]},"reference":[{"key":"2045_CR1","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1038\/s41536-018-0055-2","volume":"3","author":"T Negoro","year":"2018","unstructured":"Negoro T, Takagaki Y, Okura H, Matsuyama A. Trends in clinical trials for articular cartilage repair by cell therapy. NPJ Regen Med. 2018;3:17.","journal-title":"NPJ Regen Med"},{"issue":"3","key":"2045_CR2","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1016\/j.scr.2014.01.006","volume":"12","author":"M Serafini","year":"2014","unstructured":"Serafini M, Sacchetti B, Pievani A, Redaelli D, Remoli C, Biondi A, Riminucci M, Bianco P. Establishment of bone marrow and hematopoietic niches in vivo by reversion of chondrocyte differentiation of human bone marrow stromal cells. Stem Cell Res. 2014;12(3):659\u201372.","journal-title":"Stem Cell Res"},{"key":"2045_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.biomaterials.2015.05.013","volume":"62","author":"K Futrega","year":"2015","unstructured":"Futrega K, Palmer JS, Kinney M, Lott WB, Ungrin MD, Zandstra PW, Doran MR. The microwell-mesh: a novel device and protocol for the high throughput manufacturing of cartilage microtissues. Biomaterials. 2015;62:1\u201312.","journal-title":"Biomaterials"},{"issue":"4","key":"2045_CR4","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1302\/2046-3758.34.2000238","volume":"3","author":"JL Cook","year":"2014","unstructured":"Cook JL, Hung CT, Kuroki K, Stoker AM, Cook CR, Pfeiffer FM, Sherman SL, Stannard JP. Animal models of cartilage repair. Bone Joint Res. 2014;3(4):89\u201394.","journal-title":"Bone Joint Res"},{"issue":"1","key":"2045_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s40634-015-0037-x","volume":"3","author":"CJ Moran","year":"2016","unstructured":"Moran CJ, Ramesh A, Brama PA, O'Byrne JM, O'Brien FJ, Levingstone TJ. The benefits and limitations of animal models for translational research in cartilage repair. J Exp Orthop. 2016;3(1):1.","journal-title":"J Exp Orthop"},{"issue":"6","key":"2045_CR6","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1016\/j.joca.2018.03.006","volume":"26","author":"E Music","year":"2018","unstructured":"Music E, Futrega K, Doran MR. Sheep as a model for evaluating mesenchymal stem\/stromal cell (MSC)-based chondral defect repair. Osteoarthr Cartil. 2018;26(6):730\u201340.","journal-title":"Osteoarthr Cartil"},{"key":"2045_CR7","doi-asserted-by":"publisher","unstructured":"Futrega K, Robey PG, Klein TJ, Crawford RW, Doran MR. Micro-pellet culture reveals that bone marrow mesenchymal stromal cell (BMSC) chondrogenic induction is triggered by a single day of TGF-beta1 exposure. bioRxiv. 2019. https:\/\/doi.org\/10.1101\/853556.","DOI":"10.1101\/853556"},{"issue":"1","key":"2045_CR8","doi-asserted-by":"publisher","first-page":"29","DOI":"10.3727\/096368909X478560","volume":"19","author":"BD Markway","year":"2010","unstructured":"Markway BD, Tan GK, Brooke G, Hudson JE, Cooper-White JJ, Doran MR. Enhanced chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells in low oxygen environment micropellet cultures. Cell Transplant. 2010;19(1):29\u201342.","journal-title":"Cell Transplant"},{"issue":"1","key":"2045_CR9","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1006\/excr.1997.3858","volume":"238","author":"B Johnstone","year":"1998","unstructured":"Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res. 1998;238(1):265\u201372.","journal-title":"Exp Cell Res"},{"issue":"3","key":"2045_CR10","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1007\/s00441-015-2159-y","volume":"361","author":"BK Babur","year":"2015","unstructured":"Babur BK, Futrega K, Lott WB, Klein TJ, Cooper-White J, Doran MR. High-throughput bone and cartilage micropellet manufacture, followed by assembly of micropellets into biphasic osteochondral tissue. Cell Tissue Res. 2015;361(3):755\u201368.","journal-title":"Cell Tissue Res"},{"issue":"1","key":"2045_CR11","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1186\/ar2574","volume":"11","author":"CL Murphy","year":"2009","unstructured":"Murphy CL, Thoms BL, Vaghjiani RJ, Lafont JE. Hypoxia. HIF-mediated articular chondrocyte function: prospects for cartilage repair. Arthritis Res Ther. 2009;11(1):213.","journal-title":"Arthritis Res Ther"},{"issue":"5","key":"2045_CR12","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1002\/art.37867","volume":"65","author":"BL Thoms","year":"2013","unstructured":"Thoms BL, Dudek KA, Lafont JE, Murphy CL. Hypoxia promotes the production and inhibits the destruction of human articular cartilage. Arthritis Rheum. 2013;65(5):1302\u201312.","journal-title":"Arthritis Rheum"},{"issue":"3","key":"2045_CR13","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.bone.2005.04.040","volume":"37","author":"JC Robins","year":"2005","unstructured":"Robins JC, Akeno N, Mukherjee A, Dalal RR, Aronow BJ, Koopman P, Clemens TL. Hypoxia induces chondrocyte-specific gene expression in mesenchymal cells in association with transcriptional activation of Sox9. Bone. 2005;37(3):313\u201322.","journal-title":"Bone"},{"issue":"7","key":"2045_CR14","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1002\/term.2104","volume":"11","author":"A Berner","year":"2017","unstructured":"Berner A, Henkel J, Woodruff MA, Saifzadeh S, Kirby G, Zaiss S, Gohlke J, Reichert JC, Nerlich M, Schuetz MA, Hutmacher DW. Scaffold-cell bone engineering in a validated preclinical animal model: precursors vs differentiated cell source. J Tissue Eng Regen Med. 2017;11(7):2081\u20139.","journal-title":"J Tissue Eng Regen Med"},{"issue":"2","key":"2045_CR15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001565","volume":"3","author":"MD Ungrin","year":"2008","unstructured":"Ungrin MD, Joshi C, Nica A, Bauwens C, Zandstra PW. Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates. PLoS One. 2008;3(2):e1565.","journal-title":"PLoS One"},{"issue":"11","key":"2045_CR16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111029","volume":"9","author":"KF Chambers","year":"2014","unstructured":"Chambers KF, Mosaad EM, Russell PJ, Clements JA, Doran MR. 3D cultures of prostate cancer cells cultured in a novel high-throughput culture platform are more resistant to chemotherapeutics compared to cells cultured in monolayer. PLoS One. 2014;9(11):e111029.","journal-title":"PLoS One"},{"issue":"9","key":"2045_CR17","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1089\/hum.2010.136","volume":"21","author":"P Bianco","year":"2010","unstructured":"Bianco P, Robey PG, Saggio I, Riminucci M. \u201cMesenchymal\u201d stem cells in human bone marrow (skeletal stem cells): a critical discussion of their nature, identity, and significance in incurable skeletal disease. Hum Gene Ther. 2010;21(9):1057\u201366.","journal-title":"Hum Gene Ther"},{"issue":"3","key":"2045_CR18","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1046\/j.1365-2141.2003.04469.x","volume":"122","author":"F Deschaseaux","year":"2003","unstructured":"Deschaseaux F, Gindraux F, Saadi R, Obert L, Chalmers D, Herve P. Direct selection of human bone marrow mesenchymal stem cells using an anti-CD49a antibody reveals their CD45med,low phenotype. Br J Haematol. 2003;122(3):506\u201317.","journal-title":"Br J Haematol"},{"issue":"8","key":"2045_CR19","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1002\/jor.20402","volume":"25","author":"R Siddappa","year":"2007","unstructured":"Siddappa R, Licht R, van Blitterswijk C, de Boer J. Donor variation and loss of multipotency during in vitro expansion of human mesenchymal stem cells for bone tissue engineering. J Orthop Res. 2007;25(8):1029\u201341.","journal-title":"J Orthop Res"},{"issue":"4","key":"2045_CR20","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1080\/14653240600855905","volume":"8","author":"M Dominici","year":"2006","unstructured":"Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315\u20137.","journal-title":"Cytotherapy"},{"issue":"8","key":"2045_CR21","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1016\/j.exphem.2008.03.004","volume":"36","author":"A Sorrentino","year":"2008","unstructured":"Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp Hematol. 2008;36(8):1035\u201346.","journal-title":"Exp Hematol"},{"issue":"2","key":"2045_CR22","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1016\/j.cell.2007.08.025","volume":"131","author":"B Sacchetti","year":"2007","unstructured":"Sacchetti B, Funari A, Michienzi S, Di Cesare S, Piersanti S, Saggio I, Tagliafico E, Ferrari S, Robey PG, Riminucci M, Bianco P. Self-renewing osteoprogenitors in bone marrow sinusoids can organize a hematopoietic microenvironment. Cell. 2007;131(2):324\u201336.","journal-title":"Cell"},{"issue":"4","key":"2045_CR23","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1359\/jbmr.2003.18.4.696","volume":"18","author":"S Shi","year":"2003","unstructured":"Shi S, Gronthos S. Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J Bone Miner Res. 2003;18(4):696\u2013704.","journal-title":"J Bone Miner Res"},{"issue":"4","key":"2045_CR24","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1182\/blood.V85.4.929.bloodjournal854929","volume":"85","author":"S Gronthos","year":"1995","unstructured":"Gronthos S, Simmons PJ. The growth factor requirements of STRO-1-positive human bone marrow stromal precursors under serum-deprived conditions in vitro. Blood. 1995;85(4):929\u201340.","journal-title":"Blood"},{"issue":"7","key":"2045_CR25","doi-asserted-by":"publisher","first-page":"1717","DOI":"10.1002\/term.2699","volume":"12","author":"O Petters","year":"2018","unstructured":"Petters O, Schmidt C, Thuemmler C, Peinemann F, Zscharnack M, Somerson JS, Schulz RM. Point-of-care treatment of focal cartilage defects with selected chondrogenic mesenchymal stromal cells - an in vitro proof-of-concept study. J Tissue Eng Regen Med. 2018;12(7):1717\u201327.","journal-title":"J Tissue Eng Regen Med"},{"issue":"1","key":"2045_CR26","doi-asserted-by":"publisher","first-page":"9321","DOI":"10.1038\/s41598-018-27587-8","volume":"8","author":"M Barilani","year":"2018","unstructured":"Barilani M, Banfi F, Sironi S, Ragni E, Guillaumin S, Polveraccio F, Rosso L, Moro M, Astori G, Pozzobon M, Lazzari L. Low-affinity nerve growth factor receptor (CD271) heterogeneous expression in adult and fetal mesenchymal stromal cells. Sci Rep. 2018;8(1):9321.","journal-title":"Sci Rep"},{"issue":"1","key":"2045_CR27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171231","volume":"12","author":"C Sanjurjo-Rodriguez","year":"2017","unstructured":"Sanjurjo-Rodriguez C, Castro-Vinuelas R, Hermida-Gomez T, Fernandez-Vazquez T, Fuentes-Boquete IM, de Toro-Santos FJ, Diaz-Prado SM, Blanco-Garcia FJ. Ovine mesenchymal stromal cells: morphologic, phenotypic and functional characterization for osteochondral tissue engineering. PLoS One. 2017;12(1):e0171231.","journal-title":"PLoS One"},{"issue":"2","key":"2045_CR28","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1002\/jcp.21670","volume":"219","author":"RC McCarty","year":"2009","unstructured":"McCarty RC, Gronthos S, Zannettino AC, Foster BK, Xian CJ. Characterisation and developmental potential of ovine bone marrow derived mesenchymal stem cells. J Cell Physiol. 2009;219(2):324\u201333.","journal-title":"J Cell Physiol"},{"issue":"5","key":"2045_CR29","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1067\/mai.2001.119555","volume":"108","author":"DM Essayan","year":"2001","unstructured":"Essayan DM. Cyclic nucleotide phosphodiesterases. J Allergy Clin Immunol. 2001;108(5):671\u201380.","journal-title":"J Allergy Clin Immunol"},{"issue":"10","key":"2045_CR30","doi-asserted-by":"publisher","first-page":"1793","DOI":"10.1089\/scd.2011.0040","volume":"20","author":"MA Scott","year":"2011","unstructured":"Scott MA, Nguyen VT, Levi B, James AW. Current methods of adipogenic differentiation of mesenchymal stem cells. Stem Cells Dev. 2011;20(10):1793\u2013804.","journal-title":"Stem Cells Dev"},{"issue":"7","key":"2045_CR31","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1007\/s11626-010-9316-0","volume":"46","author":"C Rentsch","year":"2010","unstructured":"Rentsch C, Hess R, Rentsch B, Hofmann A, Manthey S, Scharnweber D, Biewener A, Zwipp H. Ovine bone marrow mesenchymal stem cells: isolation and characterization of the cells and their osteogenic differentiation potential on embroidered and surface-modified polycaprolactone-co-lactide scaffolds. In Vitro Cell Dev Biol Anim. 2010;46(7):624\u201334.","journal-title":"In Vitro Cell Dev Biol Anim"},{"issue":"3","key":"2045_CR32","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1089\/ten.2006.0128","volume":"13","author":"VL Sales","year":"2007","unstructured":"Sales VL, Mettler BA, Lopez-Ilasaca M, Johnson JA Jr, Mayer JE Jr. Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Tissue Eng. 2007;13(3):525\u201335.","journal-title":"Tissue Eng"},{"issue":"4","key":"2045_CR33","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1111\/j.1365-2184.2009.00621.x","volume":"42","author":"A Krinner","year":"2009","unstructured":"Krinner A, Zscharnack M, Bader A, Drasdo D, Galle J. Impact of oxygen environment on mesenchymal stem cell expansion and chondrogenic differentiation. Cell Prolif. 2009;42(4):471\u201384.","journal-title":"Cell Prolif"},{"issue":"2","key":"2045_CR34","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1159\/000178024","volume":"190","author":"M Zscharnack","year":"2009","unstructured":"Zscharnack M, Poesel C, Galle J, Bader A. Low oxygen expansion improves subsequent chondrogenesis of ovine bone-marrow-derived mesenchymal stem cells in collagen type I hydrogel. Cells Tissues Organs. 2009;190(2):81\u201393.","journal-title":"Cells Tissues Organs"},{"issue":"7","key":"2045_CR35","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1007\/s40273-018-0737-z","volume":"37","author":"X Armoiry","year":"2019","unstructured":"Armoiry X, Cummins E, Connock M, Metcalfe A, Royle P, Johnston R, Rodrigues J, Waugh N, Mistry H. Autologous chondrocyte implantation with chondrosphere for treating articular cartilage defects in the knee: an evidence review group perspective of a NICE single technology appraisal. Pharmacoeconomics. 2019;37(7):879\u201386.","journal-title":"Pharmacoeconomics"},{"issue":"6","key":"2045_CR36","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1089\/ten.teb.2013.0771","volume":"20","author":"RA Somoza","year":"2014","unstructured":"Somoza RA, Welter JF, Correa D, Caplan AI. Chondrogenic differentiation of mesenchymal stem cells: challenges and unfulfilled expectations. Tissue Eng Part B Rev. 2014;20(6):596\u2013608.","journal-title":"Tissue Eng Part B Rev"},{"key":"2045_CR37","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1007\/978-1-62703-989-5_21","volume":"1130","author":"PG Robey","year":"2014","unstructured":"Robey PG, Kuznetsov SA, Riminucci M, Bianco P. Bone marrow stromal cell assays: in vitro and in vivo. Methods Mol Biol. 2014;1130:279\u201393.","journal-title":"Methods Mol Biol"},{"issue":"2","key":"2045_CR38","doi-asserted-by":"publisher","first-page":"102","DOI":"10.2519\/jospt.2014.5055","volume":"44","author":"PK Edwards","year":"2014","unstructured":"Edwards PK, Ackland T, Ebert JR. Clinical rehabilitation guidelines for matrix-induced autologous chondrocyte implantation on the tibiofemoral joint. J Orthop Sports Phys Ther. 2014;44(2):102\u201319.","journal-title":"J Orthop Sports Phys Ther"},{"key":"2045_CR39","doi-asserted-by":"publisher","unstructured":"Gupta PK, Das AK, Chullikana A, Majumdar AS. Mesenchymal stem cells for cartilage repair in osteoarthritis. Stem Cell Res Ther. 2012;3(4):25. https:\/\/doi.org\/10.1186\/scrt116.","DOI":"10.1186\/scrt116"},{"issue":"4","key":"2045_CR40","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.stem.2008.03.002","volume":"2","author":"P Bianco","year":"2008","unstructured":"Bianco P, Robey PG, Simmons PJ. Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell. 2008;2(4):313\u20139.","journal-title":"Cell Stem Cell"},{"key":"2045_CR41","doi-asserted-by":"publisher","first-page":"204","DOI":"10.2174\/1874325001004010204","volume":"4","author":"RC McCarty","year":"2010","unstructured":"McCarty RC, Xian CJ, Gronthos S, Zannettino AC, Foster BK. Application of autologous bone marrow derived mesenchymal stem cells to an ovine model of growth plate cartilage injury. Open Orthop J. 2010;4:204\u201310.","journal-title":"Open Orthop J"},{"issue":"3","key":"2045_CR42","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1002\/jor.20502","volume":"26","author":"CW Jones","year":"2008","unstructured":"Jones CW, Willers C, Keogh A, Smolinski D, Fick D, Yates PJ, Kirk TB, Zheng MH. Matrix-induced autologous chondrocyte implantation in sheep: objective assessments including confocal arthroscopy. J Orthop Res. 2008;26(3):292\u2013303.","journal-title":"J Orthop Res"},{"key":"2045_CR43","doi-asserted-by":"publisher","unstructured":"Bozkurt M, A\u015f\u0131k MD, G\u00fcrsoy S, T\u00fcrk M, Karahan S, G\u00fcm\u00fc\u015fkaya B, Akkaya M, \u015eim\u015fek ME, Cay N, Do\u011fan M. Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects. Orthop Surg Res. 2019;14:394. https:\/\/doi.org\/10.1186\/s13018-019-1434-0.","DOI":"10.1186\/s13018-019-1434-0"},{"issue":"8","key":"2045_CR44","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1016\/j.joca.2005.04.001","volume":"13","author":"R Dorotka","year":"2005","unstructured":"Dorotka R, Bindreiter U, Macfelda K, Windberger U, Nehrer S. Marrow stimulation and chondrocyte transplantation using a collagen matrix for cartilage repair. Osteoarthr Cartil. 2005;13(8):655\u201364.","journal-title":"Osteoarthr Cartil"},{"issue":"1","key":"2045_CR45","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1002\/jcp.21176","volume":"214","author":"LA Kaback","year":"2008","unstructured":"Kaback LA, Soung do Y, Naik A, Smith N, Schwarz EM, O'Keefe RJ, Drissi H. Osterix\/Sp7 regulates mesenchymal stem cell mediated endochondral ossification. J Cell Physiol. 2008;214(1):173\u201382.","journal-title":"J Cell Physiol"},{"issue":"Suppl 1","key":"2045_CR46","doi-asserted-by":"publisher","first-page":"83","DOI":"10.3109\/03008207.2014.923872","volume":"55","author":"H Rashid","year":"2014","unstructured":"Rashid H, Ma C, Chen H, Wang H, Hassan MQ, Sinha K, de Crombrugghe B, Javed A. Sp7 and Runx2 molecular complex synergistically regulate expression of target genes. Connect Tissue Res. 2014;55(Suppl 1):83\u20137.","journal-title":"Connect Tissue Res"},{"issue":"18","key":"2045_CR47","doi-asserted-by":"publisher","first-page":"4625","DOI":"10.1073\/pnas.1720658115","volume":"115","author":"P Occhetta","year":"2018","unstructured":"Occhetta P, Pigeot S, Rasponi M, Dasen B, Mehrkens A, Ullrich T, Kramer I, Guth-Gundel S, Barbero A, Martin I. Developmentally inspired programming of adult human mesenchymal stromal cells toward stable chondrogenesis. Proc Natl Acad Sci U S A. 2018;115(18):4625\u201330.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"19","key":"2045_CR48","doi-asserted-by":"publisher","first-page":"3875","DOI":"10.1007\/s00018-019-03099-0","volume":"76","author":"S Diederichs","year":"2019","unstructured":"Diederichs S, Tonnier V, Marz M, Dreher SI, Geisbusch A, Richter W. Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy. Cell Mol Life Sci. 2019;76(19):3875\u201389.","journal-title":"Cell Mol Life Sci"},{"issue":"1","key":"2045_CR49","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1007\/s12178-020-09598-z","volume":"13","author":"A Arshi","year":"2020","unstructured":"Arshi A, Petrigliano FA, Williams RJ, Jones KJ. Stem cell treatment for knee articular cartilage defects and osteoarthritis. Curr Rev Musculoskelet Med. 2020;13(1):20\u20137.","journal-title":"Curr Rev Musculoskelet Med"},{"key":"2045_CR50","doi-asserted-by":"publisher","unstructured":"Lam ATL, Reuveny S, Oh SK-W. Human mesenchymal stem cell therapy for cartilage repair: review on isolation, expansion, and constructs. Stem Cell Res. 2020;44:101738. https:\/\/doi.org\/10.1016\/j.scr.2020.101738.","DOI":"10.1016\/j.scr.2020.101738"},{"issue":"6","key":"2045_CR51","doi-asserted-by":"publisher","first-page":"4789","DOI":"10.1007\/s11033-020-05428-0","volume":"47","author":"C Eder","year":"2020","unstructured":"Eder C, Schmidt-Bleek K, Geissler S, Sass FA, Maleitzke T, Pumberger M, Perka C, Duda GN, Winkler T. Mesenchymal stromal cell and bone marrow concentrate therapies for musculoskeletal indications: a concise review of current literature. Mol Biol Rep. 2020;47(6):4789\u2013814.","journal-title":"Mol Biol Rep"}],"container-title":["Stem Cell Research &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-020-02045-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13287-020-02045-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13287-020-02045-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T15:45:06Z","timestamp":1610034306000},"score":1,"resource":{"primary":{"URL":"https:\/\/stemcellres.biomedcentral.com\/articles\/10.1186\/s13287-020-02045-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,7]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["2045"],"URL":"https:\/\/doi.org\/10.1186\/s13287-020-02045-3","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.05.15.094847","asserted-by":"object"}]},"ISSN":["1757-6512"],"issn-type":[{"value":"1757-6512","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,7]]},"assertion":[{"value":"25 July 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 November 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 January 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Human bone marrow was collected from informed consenting healthy volunteer donors. Donation and use of cells were approved by the Mater Hospital Human Research Ethics Committee and the Queensland University of Technology Human Research Ethics Committee. All animal procedures were approved by the Queensland University of Technology Animal Ethics Committee.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Co-authors have no conflict of interest to report. Authors, their immediate family, and any research foundation to which they might be affiliated to did not receive any financial payments or other benefits from any commercial entity related to the subject of this article.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"26"}}