{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:22:56Z","timestamp":1780449776035,"version":"3.54.1"},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,6,19]],"date-time":"2021-06-19T00:00:00Z","timestamp":1624060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,19]],"date-time":"2021-06-19T00:00:00Z","timestamp":1624060800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DC013912-01"],"award-info":[{"award-number":["R01 DC013912-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Stem Cell Res Ther"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Hearing loss affects 25% of the population at ages 60\u201369\u2009years. Loss of the hair cells of the inner ear commonly underlies deafness and once lost this cell type cannot spontaneously regenerate in higher vertebrates. As a result, there is a need for the development of regenerative strategies to replace hair cells once lost. Stem cell-based therapies are one such strategy and offer promise for cell replacement in a variety of tissues. A number of investigators have previously demonstrated successful implantation, and certain level of regeneration of hair and supporting cells in both avian and mammalian models using rodent pluripotent stem cells. However, the ability of human stem cells to engraft and generate differentiated cell types in the inner ear is not well understood.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>We differentiate human pluripotent stem cells to the pre-placodal stage in vitro then transplant them into the mouse cochlea after selective and complete lesioning of the endogenous population of hair cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We demonstrate that hair cell ablation prior to transplantation leads to increased engraftment in the auditory sensory epithelium, the organ of Corti, as well as differentiation of transplanted cells into hair and supporting cell immunophenotypes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>We have demonstrated the feasibility of human stem cell engraftment into an ablated mouse organ of Corti.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13287-021-02403-9","type":"journal-article","created":{"date-parts":[[2021,6,19]],"date-time":"2021-06-19T09:02:41Z","timestamp":1624093361000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Selective ablation of cochlear hair cells promotes engraftment of human embryonic stem cell-derived progenitors in the mouse organ of Corti"],"prefix":"10.1186","volume":"12","author":[{"given":"Hiroki","family":"Takeda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Dondzillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica A.","family":"Randall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel P.","family":"Gubbels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,19]]},"reference":[{"key":"2403_CR1","doi-asserted-by":"publisher","first-page":"1772","DOI":"10.1126\/science.3381100","volume":"240","author":"JT Corwin","year":"1988","unstructured":"Corwin JT, Cotanche DA. Regeneration of sensory hair cells after acoustic trauma. Science (80- ). 1988;240:1772\u20134.","journal-title":"Science (80- )"},{"key":"2403_CR2","doi-asserted-by":"publisher","first-page":"1774","DOI":"10.1126\/science.3381101","volume":"240","author":"BM Ryals","year":"1988","unstructured":"Ryals BM, Rubel EW. Hair cell regeneration after acoustic trauma in adult coturnix quail. Science (80- ). 1988;240:1774\u20136.","journal-title":"Science (80- )"},{"key":"2403_CR3","doi-asserted-by":"crossref","unstructured":"Krumm B, Klump G, K\u00f6ppl C, Langemann U. Barn owls have ageless ears. Proc R Soc B Biol Sci. 2017;284(1863):20171584.\u00a0\u00a0\u00a0\u00a0\u00a0","DOI":"10.1098\/rspb.2017.1584"},{"key":"2403_CR4","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/j.tins.2018.06.008","volume":"41","author":"H Takeda","year":"2018","unstructured":"Takeda H, Dondzillo A, Randall JA, Gubbels SP. Challenges in cell-based therapies for the treatment of hearing loss. 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