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The approval number for this project is 1100306, and the date of approval is March 06, 2021. All animals involved in the study were handled in accordance with ethical guidelines, and their care and use were approved by the Medical and Laboratory Animal Ethics Committee of National Yang Ming Chiao Tung University.The use of human umbilical cord in this study was approved by the research project titled \u201cThe outcomes and mechanism of xenografting of human umbilical mesenchymal stem cells from Wharton\u2019 s jelly on mouse amyotrophic lateral sclerosis\u201d and granted by the Institutional Review Board of Taipei Veterans General Hospital. The ethics approval number for this project is 2021\u201301-009BC, and the date of approval is January 07, 2021. 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