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Human samples are derived from healthy volunteer donors who provided an informed consent. All experiments are approved by the Regional Ethical Research Committee of the Bordet Institute and conducted in accordance with the principles outlined in the Declaration of Helsinki []. The T-cells were obtained from PBMC in AP-HP, H\u00f4pital Saint-Louis, Unit\u00e9 de Th\u00e9rapie Cellulaire. All samples collected from healthy adult donors were obtained after a written and informed consent, following the Helsinki\u2019s Declaration and in compliance with the regulation of the Health Authorities. Approval was obtained from the Comit\u00e9 de Protection des Personnes (Ethics Committee) Ile de France IV (IRB00003835) []. The iPSC-MSC (iCell Mesenchymal Stem Cells Kit, 01279 #R1098) were purchased from FUJIFILM Cellular Dynamics, Inc. (FCDI). The original iPSC lines were sourced from the CIRM iPSC Repository, which contains samples from 2,500 donors. All tissue collection and informed consent procedures were conducted in accordance with CIRM guidelines and approved by Institutional Review Boards. The Human Umbilical Vein Endothelial Cells (HUVEC, #C-12200) and Human Cardiac Fibroblasts (HCF, #C-12375) were purchased from PromoCell and obtained from donors who have signed an informed consent form detailing the purpose of the donation and the tissue processing procedure. All procedures were approved by the Institutional Ethics Committee from the Universit\u00e9 de Paris (Project APAFIS#25824) on the 19\/08\/2020 and comply with the ARRIVE guidelines 2.0 and with the European legislation (EU Directive 2010\/63\/EU for animal experiments). All procedures followed approved ethical guidelines and institutional policies. The project title is: \u201c\u00c9valuation des v\u00e9sicules extracellulaires d\u00e9riv\u00e9es de cellules souches d\u2019origine humaine comme une alternative \u00e0 la greffe des cellules: applications \u00e0 la r\u00e9paration myocardique dans un mod\u00e8le d\u2019insuffisance cardiaque chronique avec validation du biomat\u00e9riau chez le rat.\u201d For this paper, we have only used the stem cell component of the application (and not the extracellular vesicles).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Human or animal rights"}},{"value":"D.S. and J.C. are scientific advisors of Revatis, a Spin Off company of the University of Liege.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"427"}}