{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:48:41Z","timestamp":1784198921378,"version":"3.55.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100008453","name":"Ontario Institute for Regenerative Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100023456","name":"Defeat Duchenne Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100023456","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR044031"],"award-info":[{"award-number":["R01AR044031"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes for Health Research","doi-asserted-by":"crossref","award":["FDN-148387"],"award-info":[{"award-number":["FDN-148387"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000098","name":"Stem Cell Network","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000098","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Skeletal Muscle"],"DOI":"10.1186\/s13395-024-00367-x","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T07:49:16Z","timestamp":1734594556000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Wnt7a is required for regeneration of dystrophic skeletal muscle"],"prefix":"10.1186","volume":"14","author":[{"given":"Uxia","family":"Gurriaran-Rodriguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasun","family":"Kodippili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Datzkiw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan","family":"Javandoost","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Rejas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Rudnicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,19]]},"reference":[{"key":"367_CR1","doi-asserted-by":"crossref","unstructured":"Bentzinger CF, von Maltzahn J, Dumont NA, Stark DA, Wang YX, Nhan K, et al. Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol. 2014;205:97\u2013111. https:\/\/rupress.org\/jcb\/article\/205\/1\/97\/37610\/Wnt7a-stimulates-myogenic-stem-cell-motility-and","DOI":"10.1083\/jcb.201310035"},{"key":"367_CR2","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/j.stem.2009.03.013","volume":"4","author":"F Le Grand","year":"2009","unstructured":"Le Grand F, Jones AE, Seale V, Scim\u00e8 A, Rudnicki MA. Wnt7a activates the Planar Cell Polarity Pathway to drive the symmetric expansion of Satellite Stem cells. Cell Stem Cell. 2009;4:535\u201347.","journal-title":"Cell Stem Cell"},{"key":"367_CR3","doi-asserted-by":"crossref","unstructured":"von Maltzahn J, Bentzinger CF, Rudnicki MA. Wnt7a-Fzd7 signalling directly activates the Akt\/mTOR anabolic growth pathway in skeletal muscle. Nat Cell Biol. 2012;14:186\u201391. http:\/\/www.nature.com.go.libproxy.wakehealth.edu\/ncb\/journal\/v14\/n2\/full\/ncb2404.html","DOI":"10.1038\/ncb2404"},{"key":"367_CR4","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1038\/nm.3990","volume":"21","author":"NA Dumont","year":"2015","unstructured":"Dumont NA, Wang YX, von Maltzahn J, Pasut A, Bentzinger CF, Brun CE, et al. Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division. Nat Med. 2015;21:1455\u201363.","journal-title":"Nat Med"},{"key":"367_CR5","unstructured":"von Maltzahn J, Renaud J-MM, Parise G, Rudnicki MA. Wnt7a treatment ameliorates muscular dystrophy. Proc Natl Acad Sci USA. 2012;109:20614\u20139. http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=3528612&tool=pmcentrez&rendertype=abstract%5Cn. https:\/\/www.scopus.com\/inward\/record.url?eid=2-s2.0-84874422456&partnerID=40&md5=9cabbb66e35a1e8f6e14ba7a7c05ddc8"},{"key":"367_CR6","doi-asserted-by":"crossref","unstructured":"Janda CY, Waghray D, Levin AM, Thomas C, Garcia KC. Structural basis of Wnt recognition by frizzled. Science. 2012;336:59\u201364.","DOI":"10.1126\/science.1222879"},{"key":"367_CR7","doi-asserted-by":"publisher","unstructured":"Gurriaran-Rodriguez U, De Repentigny Y, Kothary R, Rudnicki MA. Isolation of small extracellular vesicles from regenerating muscle tissue using tangential flow filtration and size exclusion chromatography. Skelet Muscle. 2024;14(1):22. https:\/\/doi.org\/10.1186\/s13395-024-00355-1","DOI":"10.1186\/s13395-024-00355-1"},{"key":"367_CR8","doi-asserted-by":"publisher","unstructured":"Van Niel G, D\u2019Angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 2018;19:213\u201328. https:\/\/doi.org\/10.1038\/nrm.2017.125","DOI":"10.1038\/nrm.2017.125"},{"key":"367_CR9","doi-asserted-by":"crossref","unstructured":"Kalluri R, LeBleu VS. The biology,function, and biomedical applications of exosomes. Science (1979). 2020;367.","DOI":"10.1126\/science.aau6977"},{"key":"367_CR10","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1038\/s43018-021-00272-y","volume":"2","author":"S Garc\u00eda-Silva","year":"2021","unstructured":"Garc\u00eda-Silva S, Benito-Mart\u00edn A, Nogu\u00e9s L, Hern\u00e1ndez-Barranco A, Mazariegos MS, Santos V, et al. Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism. Nat Cancer. 2021;2:1387\u2013405.","journal-title":"Nat Cancer"},{"key":"367_CR11","doi-asserted-by":"publisher","unstructured":"Gross JC, Chaudhary V, Bartscherer K, Boutros M. Active Wnt proteins are secreted on exosomes. Nat Cell Biol. 2012;14:1036\u201345. https:\/\/doi.org\/10.1038\/ncb2574","DOI":"10.1038\/ncb2574"},{"key":"367_CR12","doi-asserted-by":"crossref","unstructured":"Menck K, Klemm F, Gross JC, Pukrop T, Wenzel D, Binder C. Induction and transport of Wnt 5a during macrophage-induced malignant invasion is mediated by two types of extracellular vesicles. Oncotarget. 2013;4:2057\u201366. http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=3875769&tool=pmcentrez&rendertype=abstract","DOI":"10.18632\/oncotarget.1336"},{"key":"367_CR13","doi-asserted-by":"publisher","first-page":"1436","DOI":"10.3390\/ijms20061436","volume":"20","author":"E Dzia\u0142o","year":"2019","unstructured":"Dzia\u0142o E, Rudnik M, Koning R, Czepiel M, Tkacz K, Baj-Krzyworzeka M, et al. WNT3a and WNT5a transported by exosomes activate WNT signaling pathways in human cardiac fibroblasts. Int J Mol Sci. 2019;20:1436.","journal-title":"Int J Mol Sci"},{"key":"367_CR14","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1038\/s41580-022-00460-3","volume":"23","author":"G van Niel","year":"2022","unstructured":"van Niel G, Carter DRF, Clayton A, Lambert DW, Raposo G, Vader P. Challenges and directions in studying cell\u2013cell communication by extracellular vesicles. Nat Rev Mol Cell Biol. 2022;23:369\u201382.","journal-title":"Nat Rev Mol Cell Biol"},{"key":"367_CR15","doi-asserted-by":"crossref","unstructured":"Beckett K, Monier S, Palmer L, Alexandre C, Green H, Raposo G et al. Europe PMC Funders Group Drosophila Wingless is loaded on exosome-like vesicles but forms a gradient in an exosome-independent manner. 2015;14:82\u201396.","DOI":"10.1111\/tra.12016"},{"key":"367_CR16","doi-asserted-by":"publisher","unstructured":"Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, Wood MJA. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol. 2011;29:3\u20134. https:\/\/doi.org\/10.1038\/nbt.1807","DOI":"10.1038\/nbt.1807"},{"key":"367_CR17","doi-asserted-by":"publisher","unstructured":"Ohno S, Takanashi M, Sudo K, Ueda S, Ishikawa A, Matsuyama N et al. Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells. Molecular Therapy. 2013;21:185\u201391. https:\/\/doi.org\/10.1038\/mt.2012.180","DOI":"10.1038\/mt.2012.180"},{"key":"367_CR18","doi-asserted-by":"crossref","unstructured":"Liu Y, Li D, Liu Z, Zhou Y, Chu D, Li X, et al. Targeted exosome-mediated delivery of opioid receptor Mu siRNA for the treatment of morphine relapse. Sci Rep. 2015;5:17543. http:\/\/www.pubmedcentral.nih.gov\/articlerender.fcgi?artid=4668387&tool=pmcentrez&rendertype=abstract","DOI":"10.1038\/srep17543"},{"key":"367_CR19","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1007\/s00795-022-00321-0","volume":"55","author":"F Nakayama","year":"2022","unstructured":"Nakayama F, Miyoshi M, Kimoto A, Kawano A, Miyashita K, Kamoshida S, et al. Pancreatic cancer cell-derived exosomes induce epithelial-mesenchymal transition in human pancreatic cancer cells themselves partially via transforming growth factor \u03b21. Med Mol Morphol. 2022;55:227\u201335.","journal-title":"Med Mol Morphol"},{"key":"367_CR20","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/2044-5040-4-7","volume":"4","author":"P Pessina","year":"2014","unstructured":"Pessina P, Cabrera D, Morales MG, Riquelme CA, Guti\u00e9rrez J, Serrano AL, et al. Novel and optimized strategies for inducing fibrosis in vivo: focus on Duchenne muscular dystrophy. Skelet Muscle. 2014;4:7.","journal-title":"Skelet Muscle"},{"key":"367_CR21","doi-asserted-by":"publisher","unstructured":"Chen W, Perkins TJ, Rudnicki MA. Quantification of Muscle Satellite Stem Cell Divisions by High-Content Analysis. 2023. pp. 537\u201353. https:\/\/link.springer.com\/https:\/\/doi.org\/10.1007\/978-1-0716-2772-3_29","DOI":"10.1007\/978-1-0716-2772-3_29"},{"key":"367_CR22","doi-asserted-by":"crossref","unstructured":"Dumont NA, Rudnicki MA. Targeting muscle stem cell intrinsic defects to treat Duchenne muscular dystrophy. NPJ Regen Med. 2016;1:16006. http:\/\/www.nature.com\/articles\/npjregenmed20166","DOI":"10.1038\/npjregenmed.2016.6"},{"key":"367_CR23","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1016\/j.stem.2018.03.022","volume":"22","author":"NC Chang","year":"2018","unstructured":"Chang NC, Sincennes M-C, Chevalier FP, Brun CE, Lacaria M, Segal\u00e9s J, et al. The Dystrophin Glycoprotein Complex regulates the epigenetic activation of muscle stem cell commitment. Cell Stem Cell. 2018;22:755\u2013e7686.","journal-title":"Cell Stem Cell"},{"key":"367_CR24","doi-asserted-by":"publisher","unstructured":"Vallon M, Yuki K, Nguyen TD, Chang J, Yuan J, Siepe D, et al. A RECK-WNT7 receptor-ligand interaction enables isoform-specific regulation of Wnt bioavailability. Cell Rep. 2018;25:339\u2013349.e9. https:\/\/doi.org\/10.1016\/j.celrep.2018.09.045","DOI":"10.1016\/j.celrep.2018.09.045"},{"key":"367_CR25","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1016\/j.cell.2007.03.044","volume":"129","author":"S Kuang","year":"2007","unstructured":"Kuang S, Kuroda K, le Grand F, Rudnicki MA. Asymmetric Self-Renewal and Commitment of Satellite Stem cells in muscle. Cell. 2007;129:999\u20131010.","journal-title":"Cell"},{"key":"367_CR26","doi-asserted-by":"crossref","unstructured":"Dunlap KA, Filant J, Hayashi K, Iii EBR, Song G, Deng JM et al. Postnatal deletion of Wnt7a inhibits uterine gland morphogenesis and compromises adult fertility in mice 1. 2011;396:386\u201396.","DOI":"10.1095\/biolreprod.111.091769"},{"key":"367_CR27","doi-asserted-by":"publisher","first-page":"38379","DOI":"10.1074\/jbc.M112.374926","volume":"287","author":"U Gurriar\u00e1n-Rodr\u00edguez","year":"2012","unstructured":"Gurriar\u00e1n-Rodr\u00edguez U, Santos-Zas I, Al-Massadi O, Mosteiro CS, Beiroa D, Nogueiras R, et al. The obestatin\/GPR39 system is up-regulated by muscle injury and functions as an autocrine regenerative system. J Biol Chem. 2012;287:38379\u201389.","journal-title":"J Biol Chem"},{"key":"367_CR28","doi-asserted-by":"crossref","unstructured":"Smith LR, Barton ER. SMASH - semi-automatic muscle analysis using segmentation of histology: a MATLAB application. Skelet Muscle. 2014;4.","DOI":"10.1186\/2044-5040-4-21"},{"key":"367_CR29","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1038\/mt.2012.283","volume":"21","author":"J-H Shin","year":"2013","unstructured":"Shin J-H, Pan X, Hakim CH, Yang HT, Yue Y, Zhang K, et al. Microdystrophin ameliorates muscular dystrophy in the Canine Model of Duchenne muscular dystrophy. Mol Ther. 2013;21:750\u20137.","journal-title":"Mol Ther"},{"key":"367_CR30","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1016\/j.stem.2019.01.002","volume":"24","author":"YX Wang","year":"2019","unstructured":"Wang YX, Feige P, Brun CE, Hekmatnejad B, Dumont NA, Renaud J-M, et al. EGFR-Aurka Signaling rescues polarity and regeneration defects in Dystrophin-Deficient muscle stem cells by increasing asymmetric divisions. Cell Stem Cell. 2019;24:419\u2013e4326.","journal-title":"Cell Stem Cell"},{"key":"367_CR31","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/978-1-4939-6771-1_3","volume-title":"Primary mouse myoblast purification using magnetic cell separation BT - muscle stem cells: methods and protocols","author":"MC Sincennes","year":"2017","unstructured":"Sincennes MC, Wang YX, Rudnicki MA. In: Perdiguero E, Cornelison DDW, editors. Primary mouse myoblast purification using magnetic cell separation BT - muscle stem cells: methods and protocols. New York, NY: Springer New York; 2017. pp. 41\u201350."},{"key":"367_CR32","doi-asserted-by":"crossref","unstructured":"Th\u00e9ry C, Amigorena S, Raposo G, Clayton A. Isolation and Characterization of Exosomes from Cell Culture Supernatants. Curr Protoc Cell Biol. 2006;3:3.22.1\u20133.22.29.","DOI":"10.1002\/0471143030.cb0322s30"},{"key":"367_CR33","doi-asserted-by":"publisher","first-page":"16070","DOI":"10.18632\/oncotarget.7445","volume":"7","author":"P Carrasco-Ram\u00edrez","year":"2016","unstructured":"Carrasco-Ram\u00edrez P, Greening DW, Andr\u00e9s G, Gopal SK, Mart\u00edn-Villar E, Renart J, et al. Podoplanin is a component of extracellular vesicles that reprograms cell-derived exosomal proteins and modulates lymphatic vessel formation. Oncotarget. 2016;7:16070\u201389.","journal-title":"Oncotarget"},{"key":"367_CR34","doi-asserted-by":"crossref","unstructured":"Gurriar\u00e1n-Rodr\u00edguez U, Santos-Zas I, Gonz\u00e1lez-S\u00e1nchez J, Beiroa D, Moresi V, Mosteiro CS et al. Action of obestatin in skeletal muscle repair: stem cell expansion, muscle growth, and microenvironment remodeling. Mol Ther. 2015;23.","DOI":"10.1038\/mt.2015.40"}],"container-title":["Skeletal Muscle"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13395-024-00367-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13395-024-00367-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13395-024-00367-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T08:05:30Z","timestamp":1734595530000},"score":1,"resource":{"primary":{"URL":"https:\/\/skeletalmusclejournal.biomedcentral.com\/articles\/10.1186\/s13395-024-00367-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,19]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["367"],"URL":"https:\/\/doi.org\/10.1186\/s13395-024-00367-x","relation":{},"ISSN":["2044-5040"],"issn-type":[{"value":"2044-5040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,19]]},"assertion":[{"value":"10 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Housing, husbandry, and all experimental protocols for mice used in this study were performed in accordance with the guidelines established by the University of Ottawa Animal Care Committee, which is based on the guidelines of the Canadian Council on Animal Care. Committee\u2019s reference number: 1. OHRIe-3868 - Experimental Protocol - Genetic Mouse Models for Investigating the Molecular Mechanisms of Muscle Regeneration [Expires June 30th, 2026]. 2. OHRIb-3826 - Breeding Protocol - Genetic Mouse Models for Investigating the Molecular Mechanisms of Muscle Regeneration [Expires March 31st, 2026].","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Animal ethics approval"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"MAR is a co-inventor on patents pertaining to the use of Wnt7a to enhance muscle regeneration. The remaining authors declare no conflict of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"34"}}