{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:32:19Z","timestamp":1792715539915,"version":"4.5.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":25,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100012457","name":"Chongqing Basic and Frontier Research Project","doi-asserted-by":"publisher","award":["cstc2017jcyjA0475"],"award-info":[{"award-number":["cstc2017jcyjA0475"]}],"id":[{"id":"10.13039\/501100012457","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EJNMMI Res"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>The aim of this study was to evaluate the diagnostic efficacy of <jats:sup>18<\/jats:sup>F-fluorocholine (<jats:sup>18<\/jats:sup>F-FCH) PET\/CT for uremic hyperparathyroidism (uHPT) compared to <jats:sup>99m<\/jats:sup>Tc-sestaMIBI SPECT\/CT and ultrasonography (US).<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>A total of 17 uHPT patients with stage 5 chronic kidney disease (CKD) were prospectively enrolled. All patients underwent US, <jats:sup>99m<\/jats:sup>Tc-sestaMIBI SPECT\/CT, and <jats:sup>18<\/jats:sup>F-FCH within 2\u2009months and received surgical treatment. Visual and quantitative methods were used for image analyses. Intraoperative localization and postoperative histological results of the reference standard as well as the sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) of the three modalities were analyzed using Pearson\u2019s <jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> tests. In addition, the diagnostic efficacy of <jats:sup>18<\/jats:sup>F-FCH PET\/CT for uHPT was evaluated. The relationships between PET parameters and laboratory parameters were assessed using the Spearman correlation coefficient.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>A total of 63 parathyroid hyperplasia lesions were resected in 17 uHPT patients, and 53 lesions were detected using <jats:sup>18<\/jats:sup>F-FCH PET\/CT with no false-positive results. The sensitivity, specificity, accuracy, PPV, and NPV were 84.13%, 100%, 86.49%, 100%, and 52.38%, respectively. In comparison, the corresponding values for <jats:sup>99m<\/jats:sup>Tc-sestaMIBI SPECT\/CT and US were 63.49%, 90.91%, 67.57%, 97.56%, and 30.30% and 61.90%, 81.82%, 64.86%, 95.12%, and 27.27%, respectively. The volume of hyper-functioning parathyroid glands was significantly different between lesions positive in <jats:sup>18<\/jats:sup>F-FCH PET\/CT and negative in <jats:sup>18<\/jats:sup>F-FCH PET\/CT (mean volume 1.36 \u00b1 0.55\u2009cm<jats:sup>3<\/jats:sup> vs. 0.83 \u00b1 0.26\u2009cm<jats:sup>3<\/jats:sup>; <jats:italic>P<\/jats:italic> = 0.019). US misidentified intrathyroidal parathyroid hyperplasia as thyroid nodules in three patients, while <jats:sup>18<\/jats:sup>F-FCH PET correctly identified the anatomy. No significant associations were observed between PET parameters and laboratory parameters in uHPT.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusion<\/jats:title>\n<jats:p><jats:sup>18<\/jats:sup>F-FCH PET\/CT was more sensitive and accurate for uHPT than <jats:sup>99m<\/jats:sup>Tc-sestaMIBI SPECT\/CT and US, and had better preoperative diagnostic efficacy, particularly for lesions diagnosed as a thyroid nodule by US.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13550-019-0583-9","type":"journal-article","created":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T17:02:34Z","timestamp":1577379754000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The role of 18F-FCH PET\/CT in patients with uremic hyperparathyroidism compared with 99mTc-sestaMIBI SPECT\/CT and ultrasonography"],"prefix":"10.1186","volume":"9","author":[{"given":"Yu","family":"Xue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengming","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyi","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengjie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Pang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,26]]},"reference":[{"issue":"4","key":"583_CR1","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1016\/j.amjsurg.2018.07.030","volume":"216","author":"J Cabo","year":"2018","unstructured":"Cabo J, Gallagher KC, Baregamian N. Challenges and controversies in the surgical management of uremic hyperparathyroidism: a systematic review. Am J Surg. 2018;216(4):713\u201322.","journal-title":"Am J Surg"},{"issue":"12","key":"583_CR2","doi-asserted-by":"publisher","first-page":"1890","DOI":"10.3390\/nu10121890","volume":"10","author":"CL Lu","year":"2018","unstructured":"Lu CL, et al. The emerging role of nutritional vitamin d in secondary hyperparathyroidism in CKD. Nutrients. 2018;10(12):1890.","journal-title":"Nutrients"},{"issue":"1","key":"583_CR3","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1007\/s00423-019-01755-4","volume":"404","author":"RR Dulfer","year":"2019","unstructured":"Dulfer RR, et al. Parathyroidectomy versus cinacalcet for tertiary hyperparathyroidism; a retrospective analysis. Langenbecks Arch Surg. 2019;404(1):71\u20139.","journal-title":"Langenbecks Arch Surg"},{"issue":"5","key":"583_CR4","doi-asserted-by":"publisher","first-page":"1227","DOI":"10.1016\/j.suc.2009.06.011","volume":"89","author":"SC Pitt","year":"2009","unstructured":"Pitt SC, Sippel RS, Chen H. Secondary and tertiary hyperparathyroidism, state of the art surgical management. Surg Clin North Am. 2009;89(5):1227\u201339.","journal-title":"Surg Clin North Am"},{"issue":"7","key":"583_CR5","doi-asserted-by":"publisher","first-page":"804","DOI":"10.1002\/bjs.10554","volume":"104","author":"RR Dulfer","year":"2017","unstructured":"Dulfer RR, et al. Systematic review of surgical and medical treatment for tertiary hyperparathyroidism. Br J Surg. 2017;104(7):804\u201313.","journal-title":"Br J Surg"},{"issue":"5","key":"583_CR6","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1016\/j.amjoto.2017.06.009","volume":"38","author":"JA Tang","year":"2017","unstructured":"Tang JA, et al. Parathyroidectomy for tertiary hyperparathyroidism: a systematic review. Am J Otolaryngol. 2017;38(5):630\u20135.","journal-title":"Am J Otolaryngol"},{"issue":"1","key":"583_CR7","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.kint.2017.04.006","volume":"92","author":"M Ketteler","year":"2017","unstructured":"Ketteler M, et al. Executive summary of the 2017 KDIGO Chronic Kidney Disease-Mineral and Bone Disorder (CKD-MBD) Guideline Update: what\u2019s changed and why it matters. Kidney Int. 2017;92(1):26\u201336.","journal-title":"Kidney Int"},{"issue":"2","key":"583_CR8","first-page":"142","volume":"32","author":"SJ Bo","year":"2018","unstructured":"Bo SJ, et al. Re-operation treatment in uremic patients complicated with persistent secondary hyperparathyroidism after parathyroidectomy with autotransplantation. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018;32(2):142\u20137.","journal-title":"Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi"},{"issue":"11","key":"583_CR9","doi-asserted-by":"publisher","first-page":"2083","DOI":"10.1007\/s00259-014-2837-0","volume":"41","author":"L Lezaic","year":"2014","unstructured":"Lezaic L, et al. (1)(8)F-Fluorocholine PET\/CT for localization of hyperfunctioning parathyroid tissue in primary hyperparathyroidism: a pilot study. Eur J Nucl Med Mol Imaging. 2014;41(11):2083\u20139.","journal-title":"Eur J Nucl Med Mol Imaging"},{"issue":"1","key":"583_CR10","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.mric.2017.08.009","volume":"26","author":"SJ Kuzminski","year":"2018","unstructured":"Kuzminski SJ, Sosa JA, Hoang JK. Update in parathyroid imaging. Magn Reson Imaging Clin N Am. 2018;26(1):151\u201366.","journal-title":"Magn Reson Imaging Clin N Am"},{"issue":"3","key":"583_CR11","doi-asserted-by":"publisher","first-page":"228","DOI":"10.2478\/raon-2013-0075","volume":"48","author":"M Hodolic","year":"2014","unstructured":"Hodolic M, et al. Incidental uptake of (18)F-fluorocholine (FCH) in the head or in the neck of patients with prostate cancer. Radiol Oncol. 2014;48(3):228\u201334.","journal-title":"Radiol Oncol"},{"issue":"41","key":"583_CR12","doi-asserted-by":"publisher","first-page":"e1701","DOI":"10.1097\/MD.0000000000001701","volume":"94","author":"L Michaud","year":"2015","unstructured":"Michaud L, et al. A pilot comparison of 18F-fluorocholine PET\/CT, ultrasonography and 123I\/99mTc-sestaMIBI dual-phase dual-isotope scintigraphy in the preoperative localization of hyperfunctioning parathyroid glands in primary or secondary hyperparathyroidism: influence of thyroid anomalies. Medicine (Baltimore). 2015;94(41):e1701.","journal-title":"Medicine (Baltimore)"},{"issue":"12","key":"583_CR13","doi-asserted-by":"publisher","first-page":"e491","DOI":"10.1097\/RLU.0000000000001845","volume":"42","author":"N Thanseer","year":"2017","unstructured":"Thanseer N, et al. Comparative effectiveness of ultrasonography, 99mTc-Sestamibi, and 18F-fluorocholine PET\/CT in detecting parathyroid adenomas in patients with primary hyperparathyroidism. Clin Nucl Med. 2017;42(12):e491\u20137.","journal-title":"Clin Nucl Med"},{"issue":"1","key":"583_CR14","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.ejso.2016.09.016","volume":"43","author":"M Hocevar","year":"2017","unstructured":"Hocevar M, et al. Focused parathyroidectomy without intraoperative parathormone testing is safe after pre-operative localization with (18)F-fluorocholine PET\/CT. Eur J Surg Oncol. 2017;43(1):133\u20137.","journal-title":"Eur J Surg Oncol"},{"issue":"3","key":"583_CR15","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1007\/s00259-017-3866-2","volume":"45","author":"P Caroli","year":"2018","unstructured":"Caroli P, et al. Prognostic value of 18F-choline PET\/CT metabolic parameters in patients with metastatic castration-resistant prostate cancer treated with abiraterone or enzalutamide. Eur J Nucl Med Mol Imaging. 2018;45(3):348\u201354.","journal-title":"Eur J Nucl Med Mol Imaging"},{"issue":"4","key":"583_CR16","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1053\/j.ajkd.2013.02.366","volume":"62","author":"S Sardiwal","year":"2013","unstructured":"Sardiwal S, et al. Bone alkaline phosphatase in CKD-mineral bone disorder. Am J Kidney Dis. 2013;62(4):810\u201322.","journal-title":"Am J Kidney Dis"},{"issue":"2","key":"583_CR17","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1148\/radiol.2016160768","volume":"284","author":"WP Kluijfhout","year":"2017","unstructured":"Kluijfhout WP, et al. (18)F Fluorocholine PET\/MR imaging in patients with primary hyperparathyroidism and inconclusive conventional imaging: a prospective pilot study. Radiology. 2017;284(2):460\u20137.","journal-title":"Radiology"},{"issue":"10","key":"583_CR18","doi-asserted-by":"publisher","first-page":"1762","DOI":"10.1007\/s00259-018-3980-9","volume":"45","author":"M Beheshti","year":"2018","unstructured":"Beheshti M, et al. (18)F-Fluorocholine PET\/CT in the assessment of primary hyperparathyroidism compared with (99\u2009m)Tc-MIBI or (99\u2009m)Tc-tetrofosmin SPECT\/CT: a prospective dual-centre study in 100 patients. Eur J Nucl Med Mol Imaging. 2018;45(10):1762\u201371.","journal-title":"Eur J Nucl Med Mol Imaging"},{"issue":"11","key":"583_CR19","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1097\/MNM.0000000000000899","volume":"39","author":"M Araz","year":"2018","unstructured":"Araz M, et al. The efficacy of fluorine-18-choline PET\/CT in comparison with 99mTc-MIBI SPECT\/CT in the localization of a hyperfunctioning parathyroid gland in primary hyperparathyroidism. Nucl Med Commun. 2018;39(11):989\u201394.","journal-title":"Nucl Med Commun"},{"issue":"10116","key":"583_CR20","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/S0140-6736(17)31430-7","volume":"391","author":"JP Bilezikian","year":"2018","unstructured":"Bilezikian JP, et al. Hyperparathyroidism. Lancet. 2018;391(10116):168\u201378.","journal-title":"Lancet"},{"issue":"5","key":"583_CR21","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1007\/s11307-018-1179-x","volume":"20","author":"AA Alharbi","year":"2018","unstructured":"Alharbi AA, et al. [(18)F]Fluorocholine uptake of parathyroid adenoma is correlated with parathyroid hormone level. Mol Imaging Biol. 2018;20(5):857\u201367.","journal-title":"Mol Imaging Biol"},{"issue":"4","key":"583_CR22","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1515\/folmed-2017-0054","volume":"59","author":"AD Botushanova","year":"2017","unstructured":"Botushanova AD, Botushanov NP, Yaneva MP. Nuclear medicine methods for evaluation of abnormal parathyroid glands in patients with primary and secondary hyperparathyroidism. Folia Med (Plovdiv). 2017;59(4):396\u2013404.","journal-title":"Folia Med (Plovdiv)"},{"issue":"12","key":"583_CR23","doi-asserted-by":"publisher","first-page":"4531","DOI":"10.1210\/jc.2014-2821","volume":"99","author":"L Michaud","year":"2014","unstructured":"Michaud L, et al. Is 18F-fluorocholine-positron emission tomography\/computerized tomography a new imaging tool for detecting hyperfunctioning parathyroid glands in primary or secondary hyperparathyroidism? J Clin Endocrinol Metab. 2014;99(12):4531\u20136.","journal-title":"J Clin Endocrinol Metab"},{"issue":"4","key":"583_CR24","doi-asserted-by":"publisher","first-page":"535","DOI":"10.4103\/ijem.IJEM_707_17","volume":"22","author":"M Prabhu","year":"2018","unstructured":"Prabhu M, Damle NA. Fluorocholine PET Imaging of Parathyroid Disease. Indian J Endocrinol Metab. 2018;22(4):535\u201341.","journal-title":"Indian J Endocrinol Metab"},{"issue":"1","key":"583_CR25","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1088\/1361-6498\/aaa86f","volume":"38","author":"S Rep","year":"2018","unstructured":"Rep S, et al. (18)F-choline PET\/CT for parathyroid scintigraphy: significantly lower radiation exposure of patients in comparison to conventional nuclear medicine imaging approaches. J Radiol Prot. 2018;38(1):343\u201356.","journal-title":"J Radiol Prot"},{"issue":"1","key":"583_CR26","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1007\/s12020-019-01895-z","volume":"64","author":"S Bergeret","year":"2019","unstructured":"Bergeret S, et al. Novel PET tracers: added value for endocrine disorders. Endocrine. 2019;64(1):14\u201330.","journal-title":"Endocrine"},{"issue":"4","key":"583_CR27","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.amjoto.2011.10.010","volume":"33","author":"A Mohebati","year":"2012","unstructured":"Mohebati A, Shaha AR. Imaging techniques in parathyroid surgery for primary hyperparathyroidism. Am J Otolaryngol. 2012;33(4):457\u201368.","journal-title":"Am J Otolaryngol"},{"issue":"11","key":"583_CR28","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1507\/endocrj.EJ11-0069","volume":"58","author":"T Yabuta","year":"2011","unstructured":"Yabuta T, et al. Ultrasonographic features of intrathyroidal parathyroid adenoma causing primary hyperparathyroidism. Endocr J. 2011;58(11):989\u201394.","journal-title":"Endocr J"},{"issue":"6","key":"583_CR29","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1097\/RLU.0000000000001181","volume":"41","author":"P Lalire","year":"2016","unstructured":"Lalire P, et al. Incidental detection of oxyphilic papillary thyroid carcinoma by 18F-fluorocholine PET\/CT. Clin Nucl Med. 2016;41(6):512\u20133.","journal-title":"Clin Nucl Med"},{"issue":"2","key":"583_CR30","doi-asserted-by":"publisher","first-page":"e101","DOI":"10.1097\/RLU.0000000000002374","volume":"44","author":"RL Vija","year":"2019","unstructured":"Vija RL, et al. Incidental finding of intrathyroid metastases of prostatic cancer on 18F-choline PET\/CT. Clin Nucl Med. 2019;44(2):e101\u20133.","journal-title":"Clin Nucl Med"},{"issue":"2","key":"583_CR31","doi-asserted-by":"publisher","first-page":"133","DOI":"10.2967\/jnmt.114.145433","volume":"43","author":"AL Aziz","year":"2015","unstructured":"Aziz AL, et al. Oncocytic adenoma of thyroid incidentally detected by 18F-fluorocholine PET\/CT. J Nucl Med Technol. 2015;43(2):133\u20134.","journal-title":"J Nucl Med Technol"},{"issue":"8","key":"583_CR32","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1097\/RLU.0000000000002559","volume":"44","author":"R Ciappuccini","year":"2019","unstructured":"Ciappuccini R, et al. Thyroid incidentaloma on 18F-fluorocholine PET\/CT and 68Ga-PSMA PET\/CT revealing a medullary thyroid carcinoma. Clin Nucl Med. 2019;44(8):663\u20135.","journal-title":"Clin Nucl Med"},{"issue":"3","key":"583_CR33","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1007\/s00259-018-4123-z","volume":"46","author":"G Treglia","year":"2019","unstructured":"Treglia G, et al. Diagnostic performance of choline PET for detection of hyperfunctioning parathyroid glands in hyperparathyroidism: a systematic review and meta-analysis. Eur J Nucl Med Mol Imaging. 2019;46(3):751\u201365.","journal-title":"Eur J Nucl Med Mol Imaging"},{"issue":"2","key":"583_CR34","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1097\/RLU.0000000000000303","volume":"39","author":"F Calabria","year":"2014","unstructured":"Calabria F, Chiaravalloti A, Schillaci O. (18)F-choline PET\/CT pitfalls in image interpretation: an update on 300 examined patients with prostate cancer. Clin Nucl Med. 2014;39(2):122\u201330.","journal-title":"Clin Nucl Med"}],"container-title":["EJNMMI Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13550-019-0583-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13550-019-0583-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13550-019-0583-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T00:26:52Z","timestamp":1608856012000},"score":1,"resource":{"primary":{"URL":"https:\/\/ejnmmires.springeropen.com\/articles\/10.1186\/s13550-019-0583-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["583"],"URL":"https:\/\/doi.org\/10.1186\/s13550-019-0583-9","relation":{},"ISSN":["2191-219X"],"issn-type":[{"value":"2191-219X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"11 September 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 December 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 December 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of the First Affiliated Hospital of Chongqing Medical University and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Informed consent was obtained from all individual participants included in the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"118"}}