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In this work, label-free LC\u2013MS\/MS was used to determine the proteome differences between<jats:italic>T. spiralis<\/jats:italic>muscle larvae (ML) at the encapsulated stage and intestinal infective larvae (IIL) at the molting stage. The results showed that a total of 2885<jats:italic>T. spiralis<\/jats:italic>proteins were identified, 323 of which were differentially expressed. These proteins were involved in cuticle structural elements, regulation of cuticle synthesis, remodeling and degradation, and hormonal regulation of molting. These differential proteins were also involved in diverse intracellular pathways, such as fatty acid biosynthesis, arachidonic acid metabolism, and mucin type<jats:italic>O<\/jats:italic>-glycan biosynthesis. qPCR results showed that five<jats:italic>T. spiralis<\/jats:italic>genes (cuticle collagen 14, putative DOMON domain-containing protein, glutamine synthetase, cathepsin F and NADP-dependent isocitrate dehydrogenase) had significantly higher transcriptional levels in 10\u00a0h IIL than ML (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), which were similar to their protein expression levels, suggesting that they might be<jats:italic>T. spiralis<\/jats:italic>molting-related genes. Identification and characterization of<jats:italic>T. spiralis<\/jats:italic>molting-related proteins will be helpful for developing vaccines and new drugs against the early enteral stage of<jats:italic>T. spiralis<\/jats:italic>.<\/jats:p>","DOI":"10.1186\/s13567-019-0689-0","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T13:02:42Z","timestamp":1569243762000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Label-free quantitative proteomic analysis of molting-related proteins of Trichinella spiralis intestinal infective larvae"],"prefix":"10.1186","volume":"50","author":[{"given":"Hua Nan","family":"Ren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan Yan","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong Xu","family":"Zhuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Xia","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,23]]},"reference":[{"key":"689_CR1","doi-asserted-by":"publisher","first-page":"2194","DOI":"10.3201\/eid1712.110896","volume":"17","author":"KD Murrell","year":"2011","unstructured":"Murrell KD, Pozio E (2011) Worldwide occurrence and impact of human trichinellosis, 1986\u20132009. 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All animal experiments in this work were approved by the Institutional Life Science Ethics Committee, Zhengzhou University (No. SCXK 2017\u20130001).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"70"}}