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The aim of this study was to investigate the potential therapeutic implication of glycolytic inhibitors on<jats:italic>Echinococcus<\/jats:italic>. Our results demonstrate that at an initial concentration of 40\u00a0\u03bcM, all inhibitors of glycolysis used in the current experiment [3-bromopyruvate (3-BrPA), ornidazole, clorsulon (CLS), sodium oxamate and 2,6-dihydroxynaphthalene (NA-P<jats:sub>2<\/jats:sub>)] show considerable in vitro effects against<jats:italic>Echinococcus granulosus<\/jats:italic>protoscoleces and<jats:italic>Echinococcus multilocularis<\/jats:italic>metacestodes. Among them, 3-BrPA exhibited the highest activity which was similar to that of nitazoxanide (NTZ) and more efficacious than albendazole (ABZ). The activity of 3-BrPA was dose dependent and resulted in severe ultrastructural destructions, as visualized by electron microscopy. An additional in vivo study in mice infected with<jats:italic>E. multilocularis<\/jats:italic>metacestodes indicates a reduction in parasite weight after the twice-weekly treatment of 25\u00a0mg\/kg 3-BrPA for 6\u00a0weeks, compared to that of the untreated control. In particular, in contrast to ABZ, the administration of 25\u00a0mg\/kg 3-BrPA did not cause toxicity to the liver and kidney in mice. Similarly, at the effective dose against<jats:italic>Echinococcus<\/jats:italic>larvae, 3-BrPA showed no significant toxicity to human hepatocytes. Taken together, the results suggest that interfering with the glycolysis of the parasite may be a novel chemotherapeutical option and 3-BrPA, which exhibited a remarkable activity against<jats:italic>Echinococcus<\/jats:italic>, may be a promising potential drug against cystic echinococcosis (CE) and alveolar echinococcosis (AE).<\/jats:p>","DOI":"10.1186\/s13567-019-0710-7","type":"journal-article","created":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T06:03:03Z","timestamp":1574143383000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["In vitro and in vivo effects of 3-bromopyruvate against Echinococcus metacestodes"],"prefix":"10.1186","volume":"50","author":[{"given":"Qi","family":"Xin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8166-0358","authenticated-orcid":false,"given":"Tao","family":"Jing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,19]]},"reference":[{"key":"710_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/s40249-016-0109-x","volume":"5","author":"AM Cadavid Restrepo","year":"2016","unstructured":"Cadavid Restrepo AM, Yang YR, McManus DP, Gray DJ, Giraudoux P, Barnes TS, Williams GM, Soares Magalh\u00e3es RJ, Hamm NA, Clements AC (2016) The landscape epidemiology of echinococcoses. 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