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We found that <jats:italic>Lactobacillus johnsonii<\/jats:italic> L531, isolated from the colon of a clinically healthy weaned piglet, effectively prevented infection with <jats:italic>Salmonella enterica<\/jats:italic> serovar Infantis in a pig model. Newly weaned piglets were intragastrically administered <jats:italic>Lactobacillus johnsonii<\/jats:italic> L531 at 1.0\u2009\u00d7\u200910<jats:sup>10<\/jats:sup>\u00a0CFU\/day for 1\u00a0week before <jats:italic>S<\/jats:italic>. Infantis challenge. Pretreatment with <jats:italic>L. johnsonii<\/jats:italic> L531 lessened the severity of diarrhea and ileal inflammation in <jats:italic>S<\/jats:italic>. Infantis\u2013infected piglets. Lactobacilli were more abundant in the ileum than jejunum after <jats:italic>L. johnsonii<\/jats:italic> L531 pretreatment. Treatment with <jats:italic>L. johnsonii<\/jats:italic> L531 reduced the abundance of total bacteria in the ileal mucosa and the production of lipocalin 2 in the jejunum of piglets challenged with <jats:italic>Salmonella<\/jats:italic>. Both intestinal morphology and transmission electron microscopy results indicated that <jats:italic>L. johnsonii<\/jats:italic> L531 alleviated intestinal tissue damage following <jats:italic>S<\/jats:italic>. Infantis challenge, especially in the villus and endoplasmic reticulum (ER). ER stress induced by <jats:italic>S<\/jats:italic>. Infantis was attenuated by <jats:italic>L. johnsonii<\/jats:italic> L531 treatment. The number of CD4<jats:sup>\u2212<\/jats:sup> CCR6<jats:sup>+<\/jats:sup> T cells decreased following <jats:italic>S.<\/jats:italic> Infantis challenge, but the percentage of CCR6<jats:sup>\u2212<\/jats:sup> IFN\u03b3<jats:sup>+<\/jats:sup> T cells in peripheral blood increased. In intestinal mesenteric lymph nodes, <jats:italic>S<\/jats:italic>. Infantis increased the proportion of CCR6<jats:sup>+<\/jats:sup> IFN\u03b3<jats:sup>+<\/jats:sup> T cells, whereas <jats:italic>L. johnsonii<\/jats:italic> L531 induced an increase in the proportion of CD4<jats:sup>+<\/jats:sup> CCR6<jats:sup>+<\/jats:sup> T cells in response to <jats:italic>S<\/jats:italic>. Infantis infection. Our data thus suggest that <jats:italic>L. johnsonii<\/jats:italic> L531 contributes to the maintenance of intestinal homeostasis by modulating T-cell responses and ER stress.<\/jats:p>","DOI":"10.1186\/s13567-020-00754-4","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T13:22:45Z","timestamp":1583500965000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Anti-inflammatory effects of Lactobacillus johnsonii L531 in a pig model of Salmonella Infantis infection involves modulation of CCR6+ T cell responses and ER stress"],"prefix":"10.1186","volume":"51","author":[{"given":"Gui-Yan","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Hui","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao-Hong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2616-8277","authenticated-orcid":false,"given":"Jiu-Feng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,24]]},"reference":[{"key":"754_CR1","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1017\/S0950268813001799","volume":"142","author":"R Bassal","year":"2014","unstructured":"Bassal R, Reisfeld A, Nissan I, Agmon V, Taran D, Schemberg B, Cohen D, Shohat T (2014) Risk factors for sporadic infection with Salmonella Infantis: a matched case-control study. 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