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The parameters of protection tested were fluke burden, faecal egg count and evaluation of hepatic lesions. In vaccinated group 1 we found a significant decrease in fluke burden in comparison to both unimmunised and infected control group (37.2%; <jats:italic>p<\/jats:italic>\u2009=\u20090.002) and to vaccinated group 2 (Alhydrogel<jats:sup>\u00ae<\/jats:sup>) (27.08%; <jats:italic>p<\/jats:italic>\u2009=\u20090.016). The lower fluke burden found in G1 was accompanied by a decrease in egg output of 28.71% in comparison with the infected control group. Additionally, gross hepatic lesions found in vaccine 1 group showed a significant decrease (<jats:italic>p<\/jats:italic>\u2009=\u20090.03) in comparison with unimmunised-infected group. The serological study showed the highest level for both IgG1 and IgG2 in animals from group 1. All these data support the hypothesis of protection found in vaccine 1 group.<\/jats:p>","DOI":"10.1186\/s13567-021-00895-0","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T11:28:39Z","timestamp":1611833319000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep"],"prefix":"10.1186","volume":"52","author":[{"given":"Rafael","family":"Zafra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2983-7288","authenticated-orcid":false,"given":"Leandro","family":"Buffoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"P\u00e9rez-Caballero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"Molina-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda T.","family":"Ruiz-Campillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro","family":"Mart\u00ednez-Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J.","family":"Mart\u00ednez Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"895_CR1","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1097\/QCO.0000000000000482","volume":"31","author":"CM Webb","year":"2018","unstructured":"Webb CM, Cabada MM (2018) Recent developments in the epidemiology, diagnosis, and treatment of Fasciola infection. 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