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Lipopolysaccharide (LPS) is essential for the growth of<jats:italic>P. multocida<\/jats:italic>genotype L1 strains in chickens and specific truncations to the full length LPS structure can attenuate bacterial virulence. Here we further dissected the roles of the outer core transferase genes<jats:italic>pcgD<\/jats:italic>and<jats:italic>hptE<\/jats:italic>in bacterial resistance to duck serum, outer membrane permeability and virulence in ducks. Two<jats:italic>P. multocida<\/jats:italic>mutants, \u0394<jats:italic>pcgD<\/jats:italic>and \u0394<jats:italic>hptE<\/jats:italic>, were constructed, and silver staining confirmed that they all produced truncated LPS profiles. Inactivation of<jats:italic>pcgD<\/jats:italic>or<jats:italic>hptE<\/jats:italic>did not affect bacterial susceptibility to duck serum and outer membrane permeability but resulted in attenuated virulence in ducks to some extent. After high-dose inoculation, \u0394<jats:italic>pcgD<\/jats:italic>showed remarkably reduced colonization levels in the blood and spleen but not in the lung and liver and caused decreased injuries in the spleen and liver compared with the wild-type strain. In contrast, the \u0394<jats:italic>hptE<\/jats:italic>loads declined only in the blood, and \u0394<jats:italic>hptE<\/jats:italic>infection caused decreased splenic lesions but also induced severe hepatic lesions. Furthermore, compared with the wild-type strain, \u0394<jats:italic>pcgD<\/jats:italic>was significantly attenuated upon oral or intramuscular challenge, whereas \u0394<jats:italic>hptE<\/jats:italic>exhibited reduced virulence only upon oral infection. Therefore, the<jats:italic>pcgD<\/jats:italic>deletion caused greater virulence attenuation in ducks, indicating the critical role of<jats:italic>pcgD<\/jats:italic>in<jats:italic>P. multocida<\/jats:italic>infection establishment and survival.<\/jats:p>","DOI":"10.1186\/s13567-021-00910-4","type":"journal-article","created":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T12:02:43Z","timestamp":1614859363000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The lipopolysaccharide outer core transferase genes pcgD and hptE contribute differently to the virulence of Pasteurella multocida in ducks"],"prefix":"10.1186","volume":"52","author":[{"given":"Xinxin","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dekang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingshu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renyong","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mafeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaqiu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xumin","family":"Ou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sai","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qun","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunya","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leichang","family":"Pan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6093-353X","authenticated-orcid":false,"given":"Anchun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,3,4]]},"reference":[{"key":"910_CR1","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1128\/CMR.00024-13","volume":"26","author":"BA Wilson","year":"2013","unstructured":"Wilson BA, Ho M (2013) Pasteurella multocida: from zoonosis to cellular microbiology. 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