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Transformation is an important mechanism for the horizontal transfer of DNA, but some elements that affect the transformation process need to be further explored. Upon entering the competent state, <jats:italic>Streptococcus<\/jats:italic> species stimulate the transcription of competence-related genes that are responsible for exogenous DNA binding, uptake and processing. In this study, we performed conserved promoter motif and qRT-PCR analyses and identified CrfP as a novel murein hydrolase that is widespread in <jats:italic>S.<\/jats:italic><jats:italic>suis<\/jats:italic> and stimulated with a peptide pheromone in the competent state through a process controlled by ComX. A bioinformatics analysis revealed that CrfP consists of a CHAP hydrolase domain and two bacterial Src homology 3-binding (SH3b) domains. Further characterization showed that CrfP could be exported to extracellular bacterial cells and lytic <jats:italic>S.<\/jats:italic><jats:italic>suis<\/jats:italic> strains of different serotypes, and this finding was verified by TEM and a turbidity assay. To investigate the potential effect of CrfP in vivo, a gene-deletion mutant (\u0394<jats:italic>crfP<\/jats:italic>) was constructed. Instead of stopping the natural transformation process, the inactivation of CrfP clearly reduced the effective transformation rate. Overall, these findings provide evidence showing that CrfP is important for <jats:italic>S.<\/jats:italic><jats:italic>suis<\/jats:italic> serovar 2 competence.<\/jats:p>","DOI":"10.1186\/s13567-021-00917-x","type":"journal-article","created":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T14:03:49Z","timestamp":1616594629000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["CrfP, a fratricide protein, contributes to natural transformation in Streptococcus suis"],"prefix":"10.1186","volume":"52","author":[{"given":"Yinchu","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiale","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiankun","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyang","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihao","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3749-8557","authenticated-orcid":false,"given":"Huochun","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,24]]},"reference":[{"key":"917_CR1","doi-asserted-by":"publisher","first-page":"3296","DOI":"10.1128\/AEM.04167-13","volume":"80","author":"L Bonifait","year":"2014","unstructured":"Bonifait L, Veillette M, Letourneau V, Grenier D, Duchaine C (2014) Detection ofStreptococcussuis in bioaerosols of swine confinement buildings. 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