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Thus, this study aimed to characterize the impact of<jats:italic>L. intracellularis<\/jats:italic>on digestive function, and how vaccination mitigates these impacts. Thirty-six<jats:italic>L. intracellularis<\/jats:italic>negative barrows were assigned to treatment groups (<jats:italic>n<\/jats:italic>\u2009=\u2009 12\/trt): (1) nonvaccinated,<jats:italic>L. intracellularis<\/jats:italic>negative (NC); (2) nonvaccinated,<jats:italic>L intracellularis<\/jats:italic>challenged (PC); and (3)<jats:italic>L. intracellularis<\/jats:italic>challenged, vaccinated (Enterisol<jats:sup>\u00ae<\/jats:sup>Ileitis, Boehringer Ingelheim) 7\u00a0weeks pre-challenge (VAC). On days post-inoculation (dpi) 0 PC and VAC pigs were inoculated with<jats:italic>L. intracellularis<\/jats:italic>. From dpi 19\u201321 fecal samples were collected for apparent total tract digestibility (ATTD) and at dpi 21, pigs were euthanized for sample collection. Post-inoculation, ADG was reduced in PC pigs compared with NC (41%,<jats:italic>P<\/jats:italic>\u2009&lt;\u2009 0.001) and VAC (25%,<jats:italic>P<\/jats:italic>\u2009&lt;\u2009 0.001) pigs. Ileal gross lesion severity was greater in PC pigs compared with NC (<jats:italic>P<\/jats:italic>\u2009=\u2009 0.003) and VAC (<jats:italic>P<\/jats:italic>\u2009=\u2009 0.018) pigs. Dry matter, organic matter, nitrogen, and energy ATTD were reduced in PC pigs compared with NC pigs (<jats:italic>P<\/jats:italic>\u2009\u2264\u2009 0.001 for all). RNAscope in situ hybridization revealed abolition of sucrase-isomaltase transcript in the ileum of PC pigs compared with NC and VAC pigs (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009 0.01). Conversely, abundance of stem cell signaling markers Wnt3, Hes1, and p27<jats:sup>Kip1<\/jats:sup>were increased in PC pigs compared with NC pigs (<jats:italic>P<\/jats:italic>\u2009\u2264\u2009 0.085). Taken together, these data demonstrate that reduced digestibility during<jats:italic>L. intracellularis<\/jats:italic>challenge is partially driven by abolition of digestive machinery in lesioned tissue. Further, vaccination mitigated several of these effects, likely from lower bacterial burden and reduced disease severity.<\/jats:p>","DOI":"10.1186\/s13567-021-00958-2","type":"journal-article","created":{"date-parts":[[2021,6,19]],"date-time":"2021-06-19T11:03:43Z","timestamp":1624100623000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Lawsonia intracellularis infected enterocytes lack sucrase-isomaltase which contributes to reduced pig digestive capacity"],"prefix":"10.1186","volume":"52","author":[{"given":"Emma T.","family":"Helm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric R.","family":"Burrough","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando L.","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7283-4782","authenticated-orcid":false,"given":"Nicholas K.","family":"Gabler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,19]]},"reference":[{"key":"958_CR1","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1002\/9781119350927.ch58","volume-title":"Diseases of swine","author":"FA Vannucci","year":"2019","unstructured":"Vannucci FA, Gebhart CJ, McOrist S (2019) Proliferative enteropathy. 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