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Recently we showed that <jats:italic>Glaesserella parasuis<\/jats:italic> serotype 4 (GPS4) increased swine tracheal epithelial barrier permeability, resulting in easier bacterial translocation. Tight junction proteins (TJ) play a crucial role in maintaining the integrity and impermeability of the epithelial barrier. GPS4 decreased the expression of the TJ ZO-1 and occludin in swine tracheal epithelial cells (STEC). Furthermore, the proinflammatory cytokines IL-6, IL-8 and TNF-\u03b1 were significantly upregulated in GPS4-infected STEC, and both the MAPK and NF-\u03baB signaling pathways were activated and contributed to the expression of TNF-\u03b1. We demonstrate that the production of proinflammatory cytokines, especially TNF-\u03b1, during GPS4 infection was involved in barrier dysfunction. Additionally, animal challenge experiments confirmed that GPS4 infection downregulated TJ in the lungs of piglets and induced a severe inflammatory response. In general, <jats:italic>G. parasuis<\/jats:italic> infection downregulated the expression of TJ and induced massive secretion of proinflammatory cytokines, resulting in epithelial barrier disruption and favoring bacterial infection. This study allowed us to better understand the mechanism by which <jats:italic>G. parasuis<\/jats:italic> crosses the respiratory tract of pigs.<\/jats:p>","DOI":"10.1186\/s13567-021-01005-w","type":"journal-article","created":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T11:03:03Z","timestamp":1634814183000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Glaesserella parasuis serotype 4 HPS4-YC disrupts the integrity of the swine tracheal epithelial barrier and facilitates bacterial translocation"],"prefix":"10.1186","volume":"52","author":[{"given":"Qing","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixing","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3001-0133","authenticated-orcid":false,"given":"Hongjie","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"1005_CR1","doi-asserted-by":"publisher","first-page":"110205","DOI":"10.1016\/j.vetimm.2021.110205","volume":"234","author":"SJ Hau","year":"2021","unstructured":"Hau SJ, Eberle KC, Brockmeier SL (2021) Importance of strain selection in the generation of heterologous immunity to Glaesserella (Haemophilus) parasuis. 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