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Their control and eradication require vaccination, but\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    Rev 1, the only vaccine available, triggers anti-O-PS antibodies that interfere in the S-brucellae serodiagnosis. Since eradication and serological surveillance of the zoonotic species are priorities, Rev 1 is banned once\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    is eradicated or where it never existed, hampering\n                    <jats:italic>B. ovis<\/jats:italic>\n                    control and eradication. To develop a\n                    <jats:italic>B. ovis<\/jats:italic>\n                    specific vaccine, we investigated three\n                    <jats:italic>Brucella<\/jats:italic>\n                    live vaccine candidates lacking N-formyl-perosamine O-PS: Bov::CA\u0394\n                    <jats:italic>wadB<\/jats:italic>\n                    (CO\n                    <jats:sub>2<\/jats:sub>\n                    -independent\n                    <jats:italic>B. ovis<\/jats:italic>\n                    with truncated LPS core oligosaccharide); Rev1::\n                    <jats:italic>wbdR<\/jats:italic>\n                    \u0394\n                    <jats:italic>wbkC<\/jats:italic>\n                    (carrying N-acetylated O-PS); and H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    (\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    rough mutant with intact LPS core). After confirming their attenuation and protection against\n                    <jats:italic>B. ovis<\/jats:italic>\n                    in mice, were tested in rams for efficacy. H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    yielded similar protection to Rev 1 against\n                    <jats:italic>B. ovis<\/jats:italic>\n                    but Bov::CA\u0394\n                    <jats:italic>wadB<\/jats:italic>\n                    and Rev1::\n                    <jats:italic>wbdR<\/jats:italic>\n                    \u0394\n                    <jats:italic>wbkC<\/jats:italic>\n                    conferred no or poor protection, respectively. All H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    vaccinated rams developed a protracted antibody response in ELISA and immunoprecipitation\n                    <jats:italic>B. ovis<\/jats:italic>\n                    diagnostic tests. In contrast, all remained negative in Rose Bengal and complement fixation tests used routinely for\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    diagnosis, though some became positive in S-LPS ELISA owing to LPS core epitope reactivity. Thus, H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    is an interesting candidate for the immunoprophylaxis of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    in\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    -free areas.\n                  <\/jats:p>","DOI":"10.1186\/s13567-022-01034-z","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T06:03:14Z","timestamp":1646200994000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Brucella melitensis H38\u0394wbkF rough mutant protects against Brucella ovis in rams"],"prefix":"10.1186","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7970-4929","authenticated-orcid":false,"given":"Pilar M.","family":"Mu\u00f1oz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Conde-\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Andr\u00e9s-Barranco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda-Jes\u00fas","family":"de Miguel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaia","family":"Z\u00fa\u00f1iga-Ripa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Arag\u00f3n-Aranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Salvador-Besc\u00f3s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estrella","family":"Mart\u00ednez-G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maite","family":"Iriarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"Barber\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Vizca\u00edno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Moriy\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Blasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,2]]},"reference":[{"key":"1034_CR1","doi-asserted-by":"publisher","first-page":"249","DOI":"10.20506\/rst.32.1.2197","volume":"32","author":"J McDermott","year":"2013","unstructured":"McDermott J, Grace D, Zinsstag J (2013) Economics of brucellosis impact and control in low-income countries. 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