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As the use of serological methods increases the window of detection, the goal of this study was to investigate if it is possible to develop a differentiating serological test for antibodies against <jats:italic>Chlamydia<\/jats:italic> species in chicken sera. Focus was on <jats:italic>C. psittaci<\/jats:italic>, <jats:italic>C. gallinacea<\/jats:italic>, and two closely related species, i.e. <jats:italic>C. abortus<\/jats:italic> and <jats:italic>C. avium<\/jats:italic>. To enable differentiating serology, a bead-based Luminex suspension array was constructed, using peptides as antigens, derived from known immunoreactive <jats:italic>Chlamydia<\/jats:italic> proteins. For the majority of these peptides, species-specific seroreactivity in mammalian sera has been reported in literature. The suspension array correctly identified antibodies against various <jats:italic>Chlamydia<\/jats:italic> species in sera from experimentally infected mice, and was also able to differentiate between antibodies against <jats:italic>C. psittaci<\/jats:italic> and <jats:italic>C. gallinacea<\/jats:italic> in sera from experimentally infected chickens. In field sera, signals were difficult to interpret as insufficient sera from experimentally infected chickens were available for evaluating the seroreactivity of all peptides. Nevertheless, results of the suspension array with field sera are supported by published data on the occurrence of <jats:italic>C. gallinacea<\/jats:italic> in Dutch layers, thereby demonstrating the proof of concept of multiplex serology for Chlamydial species in poultry.<\/jats:p>","DOI":"10.1186\/s13567-023-01159-9","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:39:15Z","timestamp":1680741555000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens"],"prefix":"10.1186","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6859-1252","authenticated-orcid":false,"given":"Fimme J.","family":"van der Wal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 P.","family":"Achterberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanet A.","family":"van der Goot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemieke","family":"Dinkla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Bossers-de Vries","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conny","family":"van Solt-Smits","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Bossers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marloes","family":"Heijne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,4,4]]},"reference":[{"key":"1159_CR1","doi-asserted-by":"publisher","first-page":"461","DOI":"10.2478\/jvetres-2020-0077","volume":"64","author":"K Zareba-Marchewka","year":"2020","unstructured":"Zareba-Marchewka K, Szymanska-Czerwinska M, Niemczuk K (2020) Chlamydiae\u2014what\u2019s new? 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