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Thus, the objective of this study was to evaluate the PCR detection of non-viable <jats:italic>M. hyopneumoniae<\/jats:italic> and its residual cell-free DNA in live pigs. Pigs were inoculated with either active or inactivated <jats:italic>M. hyopneumoniae<\/jats:italic> and were sampled for up to 14\u00a0days. <jats:italic>Mycoplasma hyopneumoniae<\/jats:italic> was not detected by PCR at any timepoint in pigs inoculated with the inactivated bacterium, suggesting that in healthy pigs, the non-viable <jats:italic>M. hyopneumoniae<\/jats:italic> DNA was rapidly sensed and cleared.<\/jats:p>","DOI":"10.1186\/s13567-024-01273-2","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T22:02:14Z","timestamp":1708034534000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterizing the detection of inactivated Mycoplasma hyopneumoniae DNA in the respiratory tract of pigs"],"prefix":"10.1186","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5204-0624","authenticated-orcid":false,"given":"Albert","family":"Canturri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9685-2037","authenticated-orcid":false,"given":"Maria","family":"Pieters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"1273_CR1","volume-title":"Diseases of Swine","author":"M Pieters","year":"2019","unstructured":"Pieters M, Maes D (2019) Mycoplasmosis. 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