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It is also a very successful colonizer of mucosal surfaces. The IgM-degrading enzyme of<jats:italic>S. suis<\/jats:italic>(Ide<jats:sub><jats:italic>Ssuis<\/jats:italic><\/jats:sub>) specifically cleaves porcine IgM, which results in complement evasion. On the basis of our previous finding that Ide<jats:sub><jats:italic>Ssuis<\/jats:italic><\/jats:sub>also cleaves the IgM B cell receptor in vitro, we verified IgM B cell receptor cleavage ex vivo in whole regional lymph nodes and investigated the working hypothesis that this IgM B cell receptor cleavage results in a long-lasting impaired B cell function. The number of IgM-secreting cells was determined via ELISpot analysis after porcine peripheral blood mononuclear cells had initially been treated with different recombinant<jats:italic>S. suis<\/jats:italic>proteins and subsequently stimulated with interleukin-2 and the toll-like receptor 7\/8 ligand R848. Compared with treatment with medium or recombinant muramidase-released protein, treatment with rIde<jats:sub><jats:italic>Ssuis<\/jats:italic><\/jats:sub>but also with a cleavage-deficient variant led to a reduction in the number of IgM-secreting cells as well as the level of secreted IgM. Flow cytometry analysis confirmed that the IgM B cell receptor was cleaved only by rIde<jats:sub><jats:italic>Ssuis<\/jats:italic>,<\/jats:sub>and the receptor recovered to pretreatment levels on day 2 after treatment. Flow cytometry analysis of B and T cells incubated with fluorescein-labelled recombinant proteins revealed that different rIde<jats:sub><jats:italic>Ssuis<\/jats:italic><\/jats:sub>variants bind specifically to B cells, most prominently the cleavage-deficient variant. Our results indicate that in vitro interference of rIde<jats:sub><jats:italic>Ssuis<\/jats:italic><\/jats:sub>with the IgM B cell receptor results in long-lasting impaired IgM secretion by B cells after toll-like receptor activation. Further studies are warranted to prove that the modulation of B cell function by Ide<jats:sub><jats:italic>Ssuis<\/jats:italic><\/jats:sub>could play a role in vivo.<\/jats:p>","DOI":"10.1186\/s13567-024-01363-1","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T05:48:57Z","timestamp":1727156937000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The immunoglobulin M-degrading enzyme of Streptococcus suis (IdeSsuis) leads to long-lasting inhibition of the activation of porcine IgM-secreting B cells"],"prefix":"10.1186","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6058-9190","authenticated-orcid":false,"given":"Annika Katharina","family":"Breitfelder","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wieland","family":"Schr\u00f6dl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph Georg","family":"Baums","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gottfried","family":"Alber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,23]]},"reference":[{"key":"1363_CR1","doi-asserted-by":"crossref","unstructured":"Gottschalk M, Segura M (2019) Streptococcosis. 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