{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T23:04:31Z","timestamp":1791759871769,"version":"4.3.4"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T00:00:00Z","timestamp":1727395200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T00:00:00Z","timestamp":1727395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2021YFD1800800"],"award-info":[{"award-number":["2021YFD1800800"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172850"],"award-info":[{"award-number":["32172850"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102684"],"award-info":[{"award-number":["32102684"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Center of Technology Innovation for pigs","award":["NCTIP-XD\/C17"],"award-info":[{"award-number":["NCTIP-XD\/C17"]}]},{"name":"Chongqing Modern Agricultural Industry Technology System","award":["CQMAITS202312"],"award-info":[{"award-number":["CQMAITS202312"]}]},{"name":"Chongqing Graduate Students Research Innovation project","award":["CYS240187"],"award-info":[{"award-number":["CYS240187"]}]},{"DOI":"10.13039\/501100012459","name":"Chongqing Graduate Student Research Innovation Project","doi-asserted-by":"publisher","award":["CYS23235"],"award-info":[{"award-number":["CYS23235"]}],"id":[{"id":"10.13039\/501100012459","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Streptococcus suis<\/jats:italic> serotype 2 (SS2) is an important zoonotic pathogen that induces an NLRP3-dependent cytokine storm. NLRP3 inflammasome activation triggers not only an inflammatory response but also pyroptosis. However, the exact mechanism underlying <jats:italic>S. suis<\/jats:italic>-induced macrophage pyroptosis is not clear. Our results showed that SS2 induced the expression of pyroptosis-associated factors, including lactate dehydrogenase (LDH) release, propidium iodide (PI) uptake and GSDMD-N expression, as well as NLRP3 inflammasome activation and IL-1\u03b2 secretion. However, GSDMD deficiency and NLRP3 inhibition using MCC950 attenuated the SS2-induced expression of pyroptosis-associated factors, suggesting that SS2 induces NLRP3-GSDMD-dependent pyroptosis. Furthermore, RACK1 knockdown also reduced the expression of pyroptosis-associated factors. In addition, RACK1 knockdown downregulated the expression of NLRP3 and Pro-IL-1\u03b2 as well as the phosphorylation of P65. Surprisingly, the interaction between RACK1 and P65 was detected by co-immunoprecipitation, indicating that RACK1 induces macrophage pyroptosis by mediating the phosphorylation of P65 to promote the transcription of NLRP3 and pro-IL-1\u03b2. Similarly, NEK7 knockdown decreased the expression of pyroptosis-associated factors and ASC oligomerization. Moreover, the results of co-immunoprecipitation revealed the interaction of NEK7-RACK1-NLRP3 during SS2 infection, demonstrating that NEK7 mediates SS2-induced pyroptosis via the regulation of NLRP3 inflammasome assembly and activation. These results demonstrate the important role of RACK1 and NEK7 in SS2-induced pyroptosis. Our study provides new insight into SS2-induced cell death.<\/jats:p>","DOI":"10.1186\/s13567-024-01376-w","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T10:02:04Z","timestamp":1727431324000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["RACK1 and NEK7 mediate GSDMD-dependent macrophage pyroptosis upon Streptococcus suis infection"],"prefix":"10.1186","volume":"55","author":[{"given":"Xin","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinrong","family":"Ran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingjie","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuyi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaixiang","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianci","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3094-5059","authenticated-orcid":false,"given":"Rendong","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"key":"1376_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2024.110005","volume":"290","author":"H Wang","year":"2024","unstructured":"Wang H, Fan Q, Wang Y, Yi L, Wang Y (2024) Rethinking the control of Streptococcus suis infection: biofilm formation. Vet Microbiol 290:110005","journal-title":"Vet Microbiol"},{"key":"1376_CR2","doi-asserted-by":"publisher","first-page":"1309","DOI":"10.3389\/fmicb.2018.01309","volume":"9","author":"Z Xu","year":"2018","unstructured":"Xu Z, Chen B, Zhang Q, Liu L, Zhang A, Yang Y, Huang K, Yan S, Yu J, Sun X, Jin M (2018) Streptococcus suis 2 transcriptional regulator TstS stimulates cytokine production and bacteremia to promote streptococcal toxic shock-like syndrome. Front Microbiol 9:1309","journal-title":"Front Microbiol"},{"key":"1376_CR3","doi-asserted-by":"publisher","first-page":"2233","DOI":"10.1007\/s10482-018-1116-7","volume":"111","author":"X Xia","year":"2018","unstructured":"Xia X, Wang X, Wei X, Jiang J, Hu J (2018) Methods for the detection and characterization of Streptococcus suis: from conventional bacterial culture methods to immunosensors. Antonie Van Leeuwenhoek 111:2233\u20132247","journal-title":"Antonie Van Leeuwenhoek"},{"key":"1376_CR4","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.3201\/eid1208.060232","volume":"12","author":"C Ye","year":"2006","unstructured":"Ye C, Zhu X, Jing H, Du H, Segura M, Zheng H, Kan B, Wang L, Bai X, Zhou Y, Cui Z, Zhang S, Jin D, Sun N, Luo X, Zhang J, Gong Z, Wang X, Wang L, Sun H, Li Z, Sun Q, Liu H, Dong B, Ke C, Yuan H, Wang H, Tian K, Wang Y, Gottschalk M, Xu J (2006) Streptococcus suis sequence type 7 outbreak, Sichuan, China. Emerg Infect Dis 12:1203\u20131208","journal-title":"Emerg Infect Dis"},{"key":"1376_CR5","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/bs.ampbs.2020.12.002","volume":"78","author":"G Tram","year":"2021","unstructured":"Tram G, Jennings MP, Blackall PJ, Atack JM (2021) Streptococcus suis pathogenesis-A diverse array of virulence factors for a zoonotic lifestyle. Adv Microb Physiol 78:217\u2013257","journal-title":"Adv Microb Physiol"},{"key":"1376_CR6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007795","volume":"15","author":"L Lin","year":"2019","unstructured":"Lin L, Xu L, Lv W, Han L, Xiang Y, Fu L, Jin M, Zhou R, Chen H, Zhang A (2019) An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS). PLoS Pathog 15:e1007795","journal-title":"PLoS Pathog"},{"key":"1376_CR7","doi-asserted-by":"publisher","first-page":"eadm9190","DOI":"10.1126\/science.adm9190","volume":"384","author":"Y Li","year":"2024","unstructured":"Li Y, Hou Y, Sun Q, Zeng H, Meng F, Tian X, He Q, Shao F, Ding J (2024) Cleavage-independent activation of ancient eukaryotic gasdermins and structural mechanisms. Science 384:eadm9190","journal-title":"Science"},{"key":"1376_CR8","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/j.immuni.2024.02.011","volume":"57","author":"KE Lawlor","year":"2024","unstructured":"Lawlor KE, Murphy JM, Vince JE (2024) Gasdermin and MLKL necrotic cell death effectors: signaling and diseases. Immunity 57:429\u2013445","journal-title":"Immunity"},{"key":"1376_CR9","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1146\/annurev-immunol-081022-021207","volume":"41","author":"J Fu","year":"2023","unstructured":"Fu J, Wu H (2023) Structural mechanisms of NLRP3 inflammasome assembly and activation. Annu Rev Immunol 41:301\u2013316","journal-title":"Annu Rev Immunol"},{"key":"1376_CR10","doi-asserted-by":"publisher","first-page":"C544","DOI":"10.1152\/ajpcell.00102.2019","volume":"317","author":"Z Wu","year":"2019","unstructured":"Wu Z, Li Y, Liu Q, Liu Y, Chen L, Zhao H, Guo H, Zhu K, Zhou N, Chai TC, Shi B (2019) Pyroptosis engagement and bladder urothelial cell-derived exosomes recruit mast cells and induce barrier dysfunction of bladder urothelium after uropathogenic E. coli infection. Am J Physiol Cell Physiol 317:C544-555","journal-title":"Am J Physiol Cell Physiol"},{"key":"1376_CR11","doi-asserted-by":"publisher","first-page":"2333271","DOI":"10.1080\/21505594.2024.2333271","volume":"15","author":"Z Wang","year":"2024","unstructured":"Wang Z, Guo L, Yuan C, Zhu C, Li J, Zhong H, Mao P, Li J, Cui L, Dong J, Liu K, Meng X, Zhu G, Wang H (2024) Staphylococcus pseudintermedius induces pyroptosis of canine corneal epithelial cells by activating the ROS-NLRP3 signalling pathway. Virulence 15:2333271","journal-title":"Virulence"},{"key":"1376_CR12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2315503120","volume":"120","author":"SA Fattinger","year":"2023","unstructured":"Fattinger SA, Maurer L, Geiser P, Bernard EM, Enz U, Ganguillet S, G\u00fcl E, Kroon S, Demarco B, Mack V, Furter M, Barthel M, Pelczar P, Shao F, Broz P, Sellin ME, Hardt WD (2023) Gasdermin D is the only Gasdermin that provides protection against acute Salmonella gut infection in mice. Proc Natl Acad Sci USA 120:e2315503120","journal-title":"Proc Natl Acad Sci USA"},{"key":"1376_CR13","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/s13567-022-01027-y","volume":"53","author":"X Wang","year":"2022","unstructured":"Wang X, Liu M, Geng N, Du Y, Li Z, Gao X, Han B, Liu J, Liu Y (2022) Staphylococcus aureus mediates pyroptosis in bovine mammary epithelial cell via activation of NLRP3 inflammasome. Vet Res 53:10","journal-title":"Vet Res"},{"key":"1376_CR14","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03210-22","volume":"10","author":"S Wang","year":"2022","unstructured":"Wang S, Wang G, Tang YD, Li S, Qin L, Wang M, Yang YB (2022) Streptococcus suis Serotype 2 infection induces splenomegaly with splenocyte apoptosis. Microbiol Spectr 10:e0321022","journal-title":"Microbiol Spectr"},{"key":"1376_CR15","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1016\/j.jia.2023.09.022","volume":"23","author":"K Shi","year":"2024","unstructured":"Shi K, Li Y, Xu M, Zhang K, Gou H, Li C, Zhai S (2024) Membrane vesicles derived from Streptococcus suis serotype 2 induce cell pyroptosis in endothelial cells via the NLRP3\/Caspase-1\/GSDMD pathway. J Integr Agric 23:1338\u20131353","journal-title":"J Integr Agric"},{"key":"1376_CR16","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/1478-811X-9-22","volume":"9","author":"DR Adams","year":"2011","unstructured":"Adams DR, Ron D, Kiely PA (2011) RACK1, A multifaceted scaffolding protein: structure and function. Cell Commun Signal 9:22","journal-title":"Cell Commun Signal"},{"key":"1376_CR17","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.3390\/ijms18071453","volume":"18","author":"M Racchi","year":"2017","unstructured":"Racchi M, Buoso E, Ronfani M, Serafini MM, Galasso M, Lanni C, Corsini E (2017) Role of hormones in the regulation of RACK1 expression as a signaling checkpoint in immunosenescence. Int J Mol Sci 18:1453","journal-title":"Int J Mol Sci"},{"key":"1376_CR18","doi-asserted-by":"publisher","DOI":"10.1111\/cns.14691","volume":"30","author":"H Ni","year":"2024","unstructured":"Ni H, Kan X, Rui Q, Zhang Y, Zhai W, Zhang B, Yu Z (2024) RACK1 promotes autophagy via the PERK signaling pathway to protect against traumatic brain injury in rats. CNS Neurosci Ther 30:e14691","journal-title":"CNS Neurosci Ther"},{"key":"1376_CR19","doi-asserted-by":"publisher","first-page":"108216","DOI":"10.1016\/j.isci.2023.108216","volume":"26","author":"KN Valenzuela-Valderas","year":"2023","unstructured":"Valenzuela-Valderas KN, Farrashzadeh E, Chang YY, Shi Y, Raudonis R, Leung BM, Rohde JR, Enninga J, Cheng Z (2023) RACK1 promotes Shigella flexneri actin-mediated invasion, motility, and cell-to-cell spreading. iScience 26:108216","journal-title":"iScience"},{"key":"1376_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011872","volume":"18","author":"YY Song","year":"2024","unstructured":"Song YY, Zhang XZ, Wang BN, Cheng YK, Guo X, Zhang X, Long SR, Liu RD, Wang ZQ, Cui J (2024) A novel Trichinella spiralis serine proteinase disrupted gut epithelial barrier and mediated larval invasion through binding to RACK1 and activating MAPK\/ERK1\/2 pathway. PLoS Negl Trop Dis 18:e0011872","journal-title":"PLoS Negl Trop Dis"},{"key":"1376_CR21","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1186\/s13567-023-01195-5","volume":"54","author":"J Ran","year":"2023","unstructured":"Ran J, Yin H, Xu Y, Wang Y, Li G, Wu X, Peng L, Peng Y, Fang R (2023) RACK1 mediates NLRP3 inflammasome activation during Pasteurella multocida infection. Vet Res 54:73","journal-title":"Vet Res"},{"key":"1376_CR22","doi-asserted-by":"publisher","first-page":"eaba4733","DOI":"10.1126\/sciadv.aba4733","volume":"6","author":"Z Qu","year":"2020","unstructured":"Qu Z, Zhou J, Zhou Y, Xie Y, Jiang Y, Wu J, Luo Z, Liu G, Yin L, Zhang XL (2020) Mycobacterial EST12 activates a RACK1-NLRP3-gasdermin D pyroptosis-IL-1\u03b2 immune pathway. Sci Adv 6:eaba4733","journal-title":"Sci Adv"},{"key":"1376_CR23","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.vetmic.2019.03.019","volume":"231","author":"R Fang","year":"2019","unstructured":"Fang R, Du H, Lei G, Liu Y, Feng S, Ye C, Li N, Peng Y (2019) NLRP3 inflammasome plays an important role in caspase-1 activation and IL-1\u03b2 secretion in macrophages infected with Pasteurella multocida. Vet Microbiol 231:207\u2013213","journal-title":"Vet Microbiol"},{"key":"1376_CR24","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1038\/cr.2015.139","volume":"25","author":"W He","year":"2015","unstructured":"He W, Wan H, Hu L, Chen P, Wang X, Huang Z, Yang ZH, Zhong CQ, Han J (2015) Gasdermin D is an executor of pyroptosis and required for interleukin-1\u03b2 secretion. Cell Res 25:1285\u20131298","journal-title":"Cell Res"},{"key":"1376_CR25","doi-asserted-by":"publisher","first-page":"7885","DOI":"10.1002\/jcp.27909","volume":"234","author":"X Xia","year":"2019","unstructured":"Xia X, Wang X, Zheng Y, Jiang J, Hu J (2019) What role does pyroptosis play in microbial infection? J Cell Physiol 234:7885\u20137892","journal-title":"J Cell Physiol"},{"key":"1376_CR26","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108405","volume":"33","author":"Y Duan","year":"2020","unstructured":"Duan Y, Zhang L, Angosto-Bazarra D, Pelegr\u00edn P, N\u00fa\u00f1ez G, He Y (2020) RACK1 mediates NLRP3 inflammasome activation by promoting NLRP3 active conformation and inflammasome assembly. Cell Rep 33:108405","journal-title":"Cell Rep"},{"key":"1376_CR27","doi-asserted-by":"publisher","first-page":"6126","DOI":"10.3390\/ijms24076126","volume":"24","author":"J Xia","year":"2023","unstructured":"Xia J, Jiang S, Dong S, Liao Y, Zhou Y (2023) The role of post-translational modifications in regulation of NLRP3 inflammasome activation. Int J Mol Sci 24:6126","journal-title":"Int J Mol Sci"},{"key":"1376_CR28","doi-asserted-by":"publisher","first-page":"400","DOI":"10.3390\/microorganisms11020400","volume":"11","author":"Q Fu","year":"2023","unstructured":"Fu Q, Jiang J, Li X, Zhai Z, Wang X, Li C, Chen Q, Man C, Du L, Wang F, Chen S (2023) Activation of MyD88-dependent TLR signaling modulates immune response of the mouse heart during Pasteurella multocida infection. Microorganisms 11:400","journal-title":"Microorganisms"},{"key":"1376_CR29","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1038\/nature16959","volume":"530","author":"Y He","year":"2016","unstructured":"He Y, Zeng MY, Yang D, Motro B, N\u00fa\u00f1ez G (2016) NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530:354\u2013357","journal-title":"Nature"},{"key":"1376_CR30","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1038\/s41419-019-2157-1","volume":"10","author":"X Chen","year":"2019","unstructured":"Chen X, Liu G, Yuan Y, Wu G, Wang S, Yuan L (2019) NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-\u03baB signaling. Cell Death Dis 10:906","journal-title":"Cell Death Dis"},{"key":"1376_CR31","doi-asserted-by":"publisher","first-page":"17962","DOI":"10.1074\/jbc.RA119.009441","volume":"294","author":"F Xie","year":"2019","unstructured":"Xie F, Zan Y, Zhang Y, Zheng N, Yan Q, Zhang W, Zhang H, Jin M, Chen F, Zhang X, Liu S (2019) The cysteine protease ApdS from Streptococcus suis promotes evasion of innate immune defenses by cleaving the antimicrobial peptide cathelicidin LL-37. J Biol Chem 294:17962\u201317977","journal-title":"J Biol Chem"},{"key":"1376_CR32","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.micpath.2006.04.001","volume":"41","author":"G Chabot-Roy","year":"2006","unstructured":"Chabot-Roy G, Willson P, Segura M, Lacouture S, Gottschalk M (2006) Phagocytosis and killing of Streptococcus suis by porcine neutrophils. Microb Pathog 41:21\u201332","journal-title":"Microb Pathog"},{"key":"1376_CR33","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/j.tim.2017.02.005","volume":"25","author":"M Segura","year":"2017","unstructured":"Segura M, Fittipaldi N, Calzas C, Gottschalk M (2017) Critical Streptococcus suis virulence factors: are they all really critical? Trends Microbiol 25:585\u2013599","journal-title":"Trends Microbiol"},{"key":"1376_CR34","doi-asserted-by":"publisher","first-page":"2249779","DOI":"10.1080\/21505594.2023.2249779","volume":"14","author":"X Jiang","year":"2023","unstructured":"Jiang X, Yu G, Zhu L, Siddique A, Zhan D, Zhou L, Yue M (2023) Flanking N- and C-terminal domains of PrsA in Streptococcus suis type 2 are crucial for inducing cell death independent of TLR2 recognition. Virulence 14:2249779","journal-title":"Virulence"},{"key":"1376_CR35","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1038\/s41392-024-01801-8","volume":"9","author":"C Zhu","year":"2024","unstructured":"Zhu C, Xu S, Jiang R, Yu Y, Bian J, Zou Z (2024) The gasdermin family: emerging therapeutic targets in diseases. Signal Transduct Target Ther 9:87","journal-title":"Signal Transduct Target Ther"},{"key":"1376_CR36","doi-asserted-by":"publisher","first-page":"1521","DOI":"10.1016\/j.chom.2021.08.010","volume":"29","author":"G Luchetti","year":"2021","unstructured":"Luchetti G, Roncaioli JL, Chavez RA, Schubert AF, Kofoed EM, Reja R, Cheung TK, Liang Y, Webster JD, Lehoux I, Skippington E, Reeder J, Haley B, Tan MW, Rose CM, Newton K, Kayagaki N, Vance RE, Dixit VM (2021) Shigella ubiquitin ligase IpaH7.8 targets gasdermin D for degradation to prevent pyroptosis and enable infection. Cell Host Microbe 29:1521-1530.e10","journal-title":"Cell Host Microbe"},{"key":"1376_CR37","doi-asserted-by":"publisher","first-page":"eabq0132","DOI":"10.1126\/science.abq0132","volume":"378","author":"Q Chai","year":"2022","unstructured":"Chai Q, Yu S, Zhong Y, Lu Z, Qiu C, Yu Y, Zhang X, Zhang Y, Lei Z, Qiang L, Li BX, Pang Y, Qiu XB, Wang J, Liu CH (2022) A bacterial phospholipid phosphatase inhibits host pyroptosis by hijacking ubiquitin. Science 378:eabq0132","journal-title":"Science"},{"key":"1376_CR38","doi-asserted-by":"publisher","first-page":"1005925","DOI":"10.3389\/fimmu.2022.1005925","volume":"13","author":"G Xu","year":"2022","unstructured":"Xu G, Guo Z, Liu Y, Yang Y, Lin Y, Li C, Huang Y, Fu Q (2022) Gasdermin D protects against Streptococcus equi subsp. zooepidemicus infection through macrophage pyroptosis. Front Immunol 13:1005925","journal-title":"Front Immunol"},{"key":"1376_CR39","doi-asserted-by":"publisher","first-page":"1788","DOI":"10.3389\/fmicb.2020.01788","volume":"11","author":"L Song","year":"2020","unstructured":"Song L, Li X, Xiao Y, Huang Y, Jiang Y, Meng G, Ren Z (2020) Contribution of Nlrp3 inflammasome activation mediated by suilysin to streptococcal toxic shock-like syndrome. Front Microbiol 11:1788","journal-title":"Front Microbiol"},{"key":"1376_CR40","doi-asserted-by":"publisher","first-page":"11692","DOI":"10.3390\/ijms241411692","volume":"24","author":"X Nie","year":"2023","unstructured":"Nie X, Ma B, Liu L, Yuan X, Li M, Liu Y, Hou Y, Yang Y, Xu J, Wang Y (2023) Endoplasmic reticulum stress mediated NLRP3 inflammasome activation and pyroptosis in THP-1 macrophages infected with Bacillus Calmette-Gu\u00e9rin. Int J Mol Sci 24:11692","journal-title":"Int J Mol Sci"},{"key":"1376_CR41","doi-asserted-by":"publisher","first-page":"105351","DOI":"10.1016\/j.micinf.2024.105351","volume":"26","author":"JH Ahn","year":"2024","unstructured":"Ahn JH, Jung DH, Kim DY, Lee TS, Kim YJ, Lee YJ, Lee YJ, Seo IS, Kim WG, Cho YJ, Shin SJ, Park JH (2024) Impact of IL-1\u03b2 on lung pathology caused by Mycobacterium abscessus infection and its association with IL-17 production. Microbes Infect 26:105351","journal-title":"Microbes Infect"},{"key":"1376_CR42","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1038\/s41420-024-01966-9","volume":"10","author":"M Ambro\u017cek-Latecka","year":"2024","unstructured":"Ambro\u017cek-Latecka M, Kozlowski P, Hoser G, Bandyszewska M, Hanusek K, Nowis D, Go\u0142\u0105b J, Grzanka M, Piekie\u0142ko-Witkowska A, Schulz L, Hornung F, Deinhardt-Emmer S, Kozlowska E, Skirecki T (2024) SARS-CoV-2 and its ORF3a, E and M viroporins activate inflammasome in human macrophages and induce of IL-1\u03b1 in pulmonary epithelial and endothelial cells. Cell Death Discov 10:191","journal-title":"Cell Death Discov"},{"key":"1376_CR43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012167","volume":"20","author":"MP Wong","year":"2024","unstructured":"Wong MP, Juan EYW, Pahmeier F, Chelluri SS, Wang P, Castillo-Rojas B, Blanc SF, Biering SB, Vance RE, Harris E (2024) The inflammasome pathway is activated by dengue virus non-structural protein 1 and is protective during dengue virus infection. PLoS Pathog 20:e1012167","journal-title":"PLoS Pathog"},{"key":"1376_CR44","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1038\/s41586-021-04384-4","volume":"602","author":"W Deng","year":"2022","unstructured":"Deng W, Bai Y, Deng F, Pan Y, Mei S, Zheng Z, Min R, Wu Z, Li W, Miao R, Zhang Z, Kupper TS, Lieberman J, Liu X (2022) Streptococcal pyrogenic exotoxin B cleaves GSDMA and triggers pyroptosis. Nature 602:496\u2013502","journal-title":"Nature"},{"key":"1376_CR45","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1038\/s41419-024-06871-8","volume":"15","author":"SJ Yow","year":"2024","unstructured":"Yow SJ, Rosli SN, Hutchinson PE, Chen KW (2024) Differential signalling requirements for RIPK1-dependent pyroptosis in neutrophils and macrophages. Cell Death Dis 15:479","journal-title":"Cell Death Dis"},{"key":"1376_CR46","doi-asserted-by":"publisher","first-page":"1915","DOI":"10.1007\/s00705-022-05484-w","volume":"167","author":"Y Wang","year":"2022","unstructured":"Wang Y, Qiao X, Li Y, Yang Q, Wang L, Liu X, Wang H, Shen H (2022) Role of the receptor for activated C kinase 1 during viral infection. Arch Virol 167:1915\u20131924","journal-title":"Arch Virol"},{"key":"1376_CR47","doi-asserted-by":"publisher","first-page":"2813","DOI":"10.1111\/bph.15218","volume":"179","author":"E Buoso","year":"2022","unstructured":"Buoso E, Masi M, Long A, Chiappini C, Travelli C, Govoni S, Racchi M (2022) Ribosomes as a nexus between translation and cancer progression: focus on ribosomal Receptor for Activated C Kinase 1 (RACK1) in breast cancer. Br J Pharmacol 179:2813\u20132828","journal-title":"Br J Pharmacol"},{"key":"1376_CR48","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00596-21","volume":"95","author":"B Shue","year":"2021","unstructured":"Shue B, Chiramel AI, Cerikan B, To TH, Fr\u00f6lich S, Pederson SM, Kirby EN, Eyre NS, Bartenschlager RFW, Best SM, Beard MR (2021) Genome-wide CRISPR screen identifies RACK1 as a critical host factor for Flavivirus replication. J Virol 95:e0059621","journal-title":"J Virol"},{"key":"1376_CR49","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1038\/nature15514","volume":"526","author":"J Shi","year":"2015","unstructured":"Shi J, Zhao Y, Wang K, Shi X, Wang Y, Huang H, Zhuang Y, Cai T, Wang F, Shao F (2015) Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 526:660\u2013665","journal-title":"Nature"},{"key":"1376_CR50","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.molimm.2023.03.016","volume":"157","author":"X Wang","year":"2023","unstructured":"Wang X, Zhao Y, Wang D, Liu C, Qi Z, Tang H, Liu Y, Zhang S, Cui Y, Li Y, Liu R, Shen Y (2023) ALK-JNK signaling promotes NLRP3 inflammasome activation and pyroptosis via NEK7 during Streptococcus pneumoniae infection. Mol Immunol 157:78\u201390","journal-title":"Mol Immunol"},{"key":"1376_CR51","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1515\/med-2022-0487","volume":"17","author":"K Wu","year":"2022","unstructured":"Wu K, Peng R, Mu Q, Jiang Y, Chen J, Ming R, Zhao J, Zhang Z, Sun Y (2022) Rack1 regulates pro-inflammatory cytokines by NF-\u03baB in diabetic nephropathy. Open Med (Wars) 17:978\u2013990","journal-title":"Open Med (Wars)"}],"container-title":["Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-024-01376-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13567-024-01376-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-024-01376-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T21:03:47Z","timestamp":1727471027000},"score":1,"resource":{"primary":{"URL":"https:\/\/veterinaryresearch.biomedcentral.com\/articles\/10.1186\/s13567-024-01376-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,27]]},"references-count":51,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["1376"],"URL":"https:\/\/doi.org\/10.1186\/s13567-024-01376-w","relation":{},"ISSN":["1297-9716"],"issn-type":[{"value":"1297-9716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,27]]},"assertion":[{"value":"11 June 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 August 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"120"}}