{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:43:15Z","timestamp":1790062995481,"version":"4.0.1"},"reference-count":64,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172300"],"award-info":[{"award-number":["82172300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Long-chain fatty acid transport protein 1 (FATP1) is a member of the fatty acid transporter family. It facilitates transmembrane transport of fatty acids and participates in lipid metabolism. Lipids are essential components of the cell and organelle membranes of <jats:italic>Trichinella spiralis.<\/jats:italic> The nematode has lost the capacity to synthesise the necessary lipids de novo and has instead evolved to obtain fatty acids and their derivatives from its host. This study aims to ascertain the primary biological characteristics and roles of <jats:italic>T. spiralis<\/jats:italic> FATP1 (TsFATP1) in lipid metabolism, larval moulting, and the development of this nematode. The results show that TsFATP1 is highly expressed at enteral <jats:italic>T. spiralis<\/jats:italic> stages, mainly localised at the cuticle, the stichosome and the intrauterine embryos of the parasite. The silencing of the <jats:italic>TsFATP1<\/jats:italic> gene by TsFATP1-specific dsRNA significantly decreases the expression levels of TsFATP1 in the worm. It reduces the contents of ATP, triglycerides, total cholesterol, and phospholipids both in vitro and in vivo. RNAi inhibits lipid metabolism, moulting, and the growth of this nematode. The results demonstrate that TsFATP1 plays an essential role in lipid metabolism, moulting, and the development of <jats:italic>T. spiralis.<\/jats:italic> It could also be a target candidate for the anti-<jats:italic>Trichinella<\/jats:italic> vaccine and drugs.<\/jats:p>","DOI":"10.1186\/s13567-024-01380-0","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T12:03:13Z","timestamp":1727697793000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development"],"prefix":"10.1186","volume":"55","author":[{"given":"Yang Li","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi Qi","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen Wen","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Yi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei Hao","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin Zhuo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong Quan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8726-5498","authenticated-orcid":false,"given":"Jing","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"1380_CR1","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.fm.2016.12.012","volume":"64","author":"A Rostami","year":"2017","unstructured":"Rostami A, Gamble HR, Dupouy-Camet J, Khazan H, Bruschi F (2017) Meat sources of infection for outbreaks of human trichinellosis. Food Microbiol 64:65\u201371","journal-title":"Food Microbiol"},{"key":"1380_CR2","doi-asserted-by":"publisher","first-page":"106388","DOI":"10.1016\/j.actatropica.2022.106388","volume":"229","author":"XZ Zhang","year":"2022","unstructured":"Zhang XZ, Wang ZQ, Cui J (2022) Epidemiology of trichinellosis in the People\u2019s Republic of China during 2009\u20132020. Acta Trop 229:106388","journal-title":"Acta Trop"},{"key":"1380_CR3","doi-asserted-by":"publisher","first-page":"4707","DOI":"10.1007\/s00436-016-5240-x","volume":"115","author":"P Jiang","year":"2016","unstructured":"Jiang P, Zhang X, Wang LA, Han LH, Yang M, Duan JY, Sun GG, Qi X, Liu RD, Wang ZQ, Cui J (2016) Survey of Trichinella infection from domestic pigs in the historical endemic areas of Henan province, central China. Parasitol Res 115:4707\u20134709","journal-title":"Parasitol Res"},{"key":"1380_CR4","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1186\/s13071-022-05597-w","volume":"15","author":"YXY Xu","year":"2022","unstructured":"Xu YXY, Zhang XZ, Weng MM, Cheng YK, Liu RD, Long SR, Wang ZQ, Cui J (2022) Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge. Parasit Vectors 15:475","journal-title":"Parasit Vectors"},{"key":"1380_CR5","doi-asserted-by":"publisher","first-page":"106263","DOI":"10.1016\/j.actatropica.2021.106263","volume":"226","author":"XZ Zhang","year":"2022","unstructured":"Zhang XZ, Yue WW, Bai SJ, Hao HN, Song YY, Long SR, Liu RD, Cui J, Wang ZQ (2022) Oral immunization with attenuated Salmonella encoding an elastase elicits protective immunity against Trichinella spiralis infection. Acta Trop 226:106263","journal-title":"Acta Trop"},{"key":"1380_CR6","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1007\/s00436-015-4779-2","volume":"115","author":"RD Liu","year":"2015","unstructured":"Liu RD, Jiang P, Wen H, Duan JY, Wang LA, Li JF, Liu CY, Sun GG, Wang ZQ, Cui J (2015) Screening and characterization of early diagnostic antigens in excretory\u2013secretory proteins from Trichinella spiralis intestinal infective larvae by immunoproteomics. Parasitol Res 115:615\u2013622","journal-title":"Parasitol Res"},{"key":"1380_CR7","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1186\/s13567-019-0689-0","volume":"50","author":"HN Ren","year":"2019","unstructured":"Ren HN, Liu RD, Song YY, Zhuo TX, Guo KX, Zhang Y, Jiang P, Wang ZQ, Cui J (2019) Label-free quantitative proteomic analysis of molting-related proteins of Trichinella spiralis intestinal infective larvae. Vet Res 50:70","journal-title":"Vet Res"},{"key":"1380_CR8","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1016\/j.ijbiomac.2021.10.021","volume":"192","author":"HN Ren","year":"2021","unstructured":"Ren HN, Bai SJ, Wang Z, Han LL, Yan SW, Jiang P, Zhang X, Wang ZQ, Cui J (2021) A metalloproteinase Tsdpy31 from Trichinella spiralis participates in larval molting and development. Int J Biol Macromol 192:883\u2013894","journal-title":"Int J Biol Macromol"},{"key":"1380_CR9","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/S0169-4758(98)01287-3","volume":"14","author":"DD Despommier","year":"1998","unstructured":"Despommier DD (1998) How does Trichinella spiralis make itself at home? Parasitol Today 14:318\u2013323","journal-title":"Parasitol Today"},{"key":"1380_CR10","doi-asserted-by":"publisher","first-page":"e349","DOI":"10.1371\/journal.pbio.0030349","volume":"3","author":"J Ewer","year":"2005","unstructured":"Ewer J (2005) How the ecdysozoan changed its coat. PLoS Biol 3:e349","journal-title":"PLoS Biol"},{"key":"1380_CR11","doi-asserted-by":"publisher","first-page":"395","DOI":"10.2307\/3276598","volume":"53","author":"WC Campbell","year":"1967","unstructured":"Campbell WC (1967) Distribution of Trichinella spiralis in the small intestine of young mice. J Parasitol 53:395\u2013397","journal-title":"J Parasitol"},{"key":"1380_CR12","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/0005-2736(96)00022-3","volume":"1281","author":"K Gounaris","year":"1996","unstructured":"Gounaris K, Smith VP, Selkirk ME (1996) Structural organisation and lipid composition of the epicuticular accessory layer of infective larvae of Trichinella spiralis. Biochim Biophys Acta 1281:91\u2013100","journal-title":"Biochim Biophys Acta"},{"key":"1380_CR13","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1111\/mmi.13010","volume":"97","author":"S Ramakrishnan","year":"2015","unstructured":"Ramakrishnan S, Docampo MD, MacRae JI, Ralton JE, Rupasinghe T, McConville MJ, Striepen B (2015) The intracellular parasite Toxoplasma gondii depends on the synthesis of long-chain and very long-chain unsaturated fatty acids not supplied by the host cell. Mol Microbiol 97:64\u201376","journal-title":"Mol Microbiol"},{"key":"1380_CR14","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1016\/j.mam.2012.07.010","volume":"34","author":"CM Anderson","year":"2013","unstructured":"Anderson CM, Stahl A (2013) SLC27 fatty acid transport proteins. Mol Aspects Med 34:516\u2013528","journal-title":"Mol Aspects Med"},{"key":"1380_CR15","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1016\/j.bbalip.2011.09.010","volume":"1821","author":"M Kazantzis","year":"2012","unstructured":"Kazantzis M, Stahl A (2012) Fatty acid transport proteins, implications in physiology and disease. Biochim Biophys Acta 1821:852\u2013857","journal-title":"Biochim Biophys Acta"},{"key":"1380_CR16","doi-asserted-by":"publisher","first-page":"e8857","DOI":"10.1371\/journal.pone.0008857","volume":"5","author":"E Kage-Nakadai","year":"2010","unstructured":"Kage-Nakadai E, Kobuna H, Kimura M, Gengyo-Ando K, Inoue T, Arai H, Mitani S (2010) Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans. PLoS ONE 5:e8857","journal-title":"PLoS ONE"},{"key":"1380_CR17","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1186\/s13071-023-05836-8","volume":"16","author":"F Wu","year":"2023","unstructured":"Wu F, Wei H, Chen X, Du Z, Huang Y, Shi H, Yang Y, Du A, Ma G (2023) Fatty acid- and retinol-binding protein 6 does not control worm fatty acid content in Caenorhabditis elegans but might play a role in Haemonchus contortus parasitism. Parasit Vectors 16:230","journal-title":"Parasit Vectors"},{"key":"1380_CR18","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1038\/ng.769","volume":"43","author":"M Mitreva","year":"2011","unstructured":"Mitreva M, Jasmer DP, Zarlenga DS, Wang Z, Abubucker S, Martin J, Taylor CM, Yin Y, Fulton L, Minx P, Yang SP, Warren WC, Fulton RS, Bhonagiri V, Zhang X, Hallsworth-Pepin K, Clifton SW, McCarter JP, Appleton J, Mardis ER, Wilson RK (2011) The draft genome of the parasitic nematode Trichinella spiralis. Nat Genet 43:228\u2013235","journal-title":"Nat Genet"},{"key":"1380_CR19","doi-asserted-by":"publisher","first-page":"e0265013","DOI":"10.1371\/journal.pone.0265013","volume":"17","author":"C Thawornkuno","year":"2022","unstructured":"Thawornkuno C, Nogrado K, Adisakwattana P, Thiangtrongjit T, Reamtong O (2022) Identification and profiling of Trichinella spiralis circulating antigens and proteins in sera of mice with trichinellosis. PLoS One 17:e0265013","journal-title":"PLoS ONE"},{"key":"1380_CR20","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1186\/s13567-023-01217-2","volume":"54","author":"Z Wang","year":"2023","unstructured":"Wang Z, Lu QQ, Weng MM, Li YL, Han LL, Song YY, Shi YL, Liu RD, Cui J, Wang ZQ (2023) Binding of Trichinella spiralis C-type lectin with syndecan-1 on intestinal epithelial cells mediates larval invasion of intestinal epithelium. Vet Res 54:86","journal-title":"Vet Res"},{"key":"1380_CR21","doi-asserted-by":"publisher","first-page":"e0011629","DOI":"10.1371\/journal.pntd.0011629","volume":"17","author":"YY Song","year":"2023","unstructured":"Song YY, Zhang XZ, Wang BN, Weng MM, Zhang ZY, Guo X, Zhang X, Wang ZQ, Cui J (2023) Molecular characterization of a novel serine proteinase from Trichinella spiralis and its participation in larval invasion of gut epithelium. PLoS Negl Trop Dis 17:e0011629","journal-title":"PLoS Negl Trop Dis"},{"key":"1380_CR22","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/s13567-020-00877-8","volume":"52","author":"CX Hu","year":"2021","unstructured":"Hu CX, Zeng J, Hao HN, Xu YXY, Liu F, Liu RD, Long SR, Wang ZQ, Cui J (2021) Biological properties and roles of a Trichinella spiralis inorganic pyrophosphatase in molting and developmental process of intestinal larval stages. Vet Res 52:6","journal-title":"Vet Res"},{"key":"1380_CR23","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1186\/s13567-020-00805-w","volume":"51","author":"KX Guo","year":"2020","unstructured":"Guo KX, Bai Y, Ren HN, Sun XY, Song YY, Liu RD, Long SR, Zhang X, Jiang P, Wang ZQ, Cui J (2020) Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity. Vet Res 51:78","journal-title":"Vet Res"},{"key":"1380_CR24","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1186\/s13071-020-3981-y","volume":"13","author":"CX Hu","year":"2020","unstructured":"Hu CX, Jiang P, Yue X, Zeng J, Zhang XZ, Song YY, Liu RD, Zhang X, Wang ZQ, Cui J (2020) Molecular characterization of a Trichinella spiralis elastase-1 and its potential as a diagnostic antigen for trichinellosis. Parasit Vectors 13:97","journal-title":"Parasit Vectors"},{"key":"1380_CR25","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/s13071-023-06067-7","volume":"17","author":"LL Han","year":"2024","unstructured":"Han LL, Lu QQ, Li YL, Zheng WW, Ren P, Liu RD, Cui J, Wang ZQ (2024) Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis. Parasit Vectors 17:9","journal-title":"Parasit Vectors"},{"key":"1380_CR26","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1186\/s13567-023-01209-2","volume":"54","author":"SW Yan","year":"2023","unstructured":"Yan SW, Zhang R, Guo X, Wang BN, Long SR, Liu RD, Wang ZQ, Cui J (2023) Trichinella spiralis dipeptidyl peptidase 1 suppressed macrophage cytotoxicity by promoting M2 polarization via the STAT6\/PPAR\u03b3 pathway. Vet Res 54:77","journal-title":"Vet Res"},{"key":"1380_CR27","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1186\/s13567-018-0555-5","volume":"49","author":"GG Sun","year":"2018","unstructured":"Sun GG, Ren HN, Liu RD, Song YY, Qi X, Hu CX, Yang F, Jiang P, Zhang X, Wang ZQ, Cui J (2018) Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection. Vet Res 49:59","journal-title":"Vet Res"},{"key":"1380_CR28","doi-asserted-by":"publisher","first-page":"106112","DOI":"10.1016\/j.actatropica.2021.106112","volume":"224","author":"Y Bai","year":"2021","unstructured":"Bai Y, Ma KN, Sun XY, Dan Liu R, Long SR, Jiang P, Wang ZQ, Cui J (2021) Molecular characterization of a novel cathepsin L from Trichinella spiralis and its participation in invasion, development and reproduction. Acta Trop 224:106112","journal-title":"Acta Trop"},{"key":"1380_CR29","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s13567-020-00767-z","volume":"51","author":"XZ Zhang","year":"2020","unstructured":"Zhang XZ, Sun XY, Bai Y, Song YY, Hu CX, Li X, Cui J, Wang ZQ (2020) Protective immunity in mice vaccinated with a novel elastase-1 significantly decreases Trichinella spiralis fecundity and infection. Vet Res 51:43","journal-title":"Vet Res"},{"key":"1380_CR30","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1186\/s13567-018-0573-3","volume":"49","author":"J Xu","year":"2018","unstructured":"Xu J, Yang F, Yang DQ, Jiang P, Liu RD, Zhang X, Cui J, Wang ZQ (2018) Molecular characterization of Trichinella spiralis galectin and its participation in larval invasion of host\u2019s intestinal epithelial cells. Vet Res 49:79","journal-title":"Vet Res"},{"key":"1380_CR31","doi-asserted-by":"publisher","first-page":"e0011874","DOI":"10.1371\/journal.pntd.0011874","volume":"18","author":"LL Han","year":"2024","unstructured":"Han LL, Lu QQ, Zheng WW, Li YL, Song YY, Zhang XZ, Long SR, Liu RD, Wang ZQ, Cui J (2024) A novel trypsin of Trichinella spiralis mediates larval invasion of gut epithelium via binding to PAR2 and activating ERK1\/2 pathway. PLoS Negl Trop Dis 18:e0011874","journal-title":"PLoS Negl Trop Dis"},{"key":"1380_CR32","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.actatropica.2015.07.002","volume":"150","author":"RD Liu","year":"2015","unstructured":"Liu RD, Cui J, Liu XL, Jiang P, Sun GG, Zhang X, Long SR, Wang L, Wang ZQ (2015) Comparative proteomic analysis of surface proteins of Trichinella spiralis muscle larvae and intestinal infective larvae. Acta Trop 150:79\u201386","journal-title":"Acta Trop"},{"key":"1380_CR33","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/s13567-023-01246-x","volume":"54","author":"KN Ma","year":"2023","unstructured":"Ma KN, Zhang Y, Zhang ZY, Wang BN, Song YY, Han LL, Zhang XZ, Long SR, Cui J, Wang ZQ (2023) Trichinella spiralis galectin binding to toll-like receptor 4 induces intestinal inflammation and mediates larval invasion of gut mucosa. Vet Res 54:113","journal-title":"Vet Res"},{"key":"1380_CR34","doi-asserted-by":"publisher","first-page":"105825","DOI":"10.1016\/j.actatropica.2021.105825","volume":"216","author":"HN Ren","year":"2021","unstructured":"Ren HN, Zhuo TX, Bai SJ, Bai Y, Sun XY, Dan Liu R, Long SR, Cui J, Wang ZQ (2021) Proteomic analysis of hydrolytic proteases in excretory\/secretory proteins from Trichinella spiralis intestinal infective larvae using zymography combined with shotgun LC-MS\/MS approach. Acta Trop 216:105825","journal-title":"Acta Trop"},{"key":"1380_CR35","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1186\/s13071-019-3833-9","volume":"12","author":"J Cui","year":"2019","unstructured":"Cui J, Han Y, Yue X, Liu F, Song YY, Yan SW, Lei JJ, Zhang X, Jiang P, Wang ZQ (2019) Vaccination of mice with a recombinant novel cathepsin B inhibits Trichinella spiralis development, reduces the fecundity and worm burden. Parasit Vectors 12:518","journal-title":"Parasit Vectors"},{"key":"1380_CR36","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s13567-018-0582-2","volume":"49","author":"JF Li","year":"2018","unstructured":"Li JF, Guo KX, Qi X, Lei JJ, Han Y, Yan SW, Jiang P, Yu C, Cheng XC, Wang ZQ, Cui J (2018) Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA. Vet Res 49:87","journal-title":"Vet Res"},{"key":"1380_CR37","doi-asserted-by":"publisher","first-page":"e0009865","DOI":"10.1371\/journal.pntd.0009865","volume":"15","author":"CX Hu","year":"2021","unstructured":"Hu CX, Xu YXY, Hao HN, Liu RD, Jiang P, Long SR, Wang ZQ, Cui J (2021) Oral vaccination with recombinant Lactobacillus plantarum encoding Trichinella spiralis inorganic pyrophosphatase elicited a protective immunity in BALB\/c mice. PLoS Negl Trop Dis 15:e0009865","journal-title":"PLoS Negl Trop Dis"},{"key":"1380_CR38","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1186\/s13567-021-00983-1","volume":"52","author":"YY Hu","year":"2021","unstructured":"Hu YY, Zhang R, Yan SW, Yue WW, Zhang JH, Liu RD, Long SR, Cui J, Wang ZQ (2021) Characterization of a novel cysteine protease in Trichinella spiralis and its role in larval intrusion, development and fecundity. Vet Res 52:113","journal-title":"Vet Res"},{"key":"1380_CR39","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1186\/s13567-021-00921-1","volume":"52","author":"Y Gao","year":"2021","unstructured":"Gao Y, Meng X, Yang X, Meng S, Han C, Li X, Wang S, Li W, Song M (2021) RNAi-mediated silencing of Trichinella spiralis glutaminase results in reduced muscle larval infectivity. Vet Res 52:51","journal-title":"Vet Res"},{"key":"1380_CR40","doi-asserted-by":"publisher","first-page":"107076","DOI":"10.1016\/j.actatropica.2023.107076","volume":"249","author":"SW Yan","year":"2024","unstructured":"Yan SW, Cheng YK, Lu QQ, Zhang R, Dan Liu R, Long SR, Wang ZQ, Cui J (2024) Characterization of a novel dipeptidyl peptidase 1 of Trichinella spiralis and its participation in larval invasion. Acta Trop 249:107076","journal-title":"Acta Trop"},{"key":"1380_CR41","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1186\/s13567-022-01104-2","volume":"53","author":"HN Hao","year":"2022","unstructured":"Hao HN, Song YY, Ma KN, Wang BN, Long SR, Liu RD, Zhang X, Wang ZQ, Cui J (2022) A novel C-type lectin from Trichinella spiralis mediates larval invasion of host intestinal epithelial cells. Vet Res 53:85","journal-title":"Vet Res"},{"key":"1380_CR42","doi-asserted-by":"publisher","first-page":"e0010881","DOI":"10.1371\/journal.pntd.0010881","volume":"16","author":"WW Yue","year":"2022","unstructured":"Yue WW, Yan SW, Zhang R, Cheng YK, Liu RD, Long SR, Zhang X, Wang ZQ, Cui J (2022) Characterization of a novel pyruvate kinase from Trichinella spiralis and its participation in sugar metabolism, larval molting and development. PLoS Negl Trop Dis 16:e0010881","journal-title":"PLoS Negl Trop Dis"},{"key":"1380_CR43","doi-asserted-by":"publisher","first-page":"e1011893","DOI":"10.1371\/journal.ppat.1011893","volume":"20","author":"XM Sun","year":"2024","unstructured":"Sun XM, Hao CY, Wu AQ, Luo ZN, El-Ashram S, Alouffi A, Gu Y, Liu S, Huang JJ, Zhu XP (2024) Trichinella spiralis-induced immunomodulation signatures on gut microbiota and metabolic pathways in mice. PLoS Pathog 20:e1011893","journal-title":"PLoS Pathog"},{"key":"1380_CR44","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1007\/978-1-0716-2181-3_14","volume":"2468","author":"HH Harrison","year":"2022","unstructured":"Harrison HH, Watts JL (2022) Lipid extraction and analysis. Methods Mol Biol 2468:271\u2013281","journal-title":"Methods Mol Biol"},{"key":"1380_CR45","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1186\/s13071-016-1704-1","volume":"9","author":"X Guo","year":"2016","unstructured":"Guo X, Zhang H, Zheng X, Zhou Q, Yang Y, Chen X, Du A (2016) Structural and functional characterization of a novel gene, Hc-daf-22, from the strongylid nematode Haemonchus contortus. Parasit Vectors 9:442","journal-title":"Parasit Vectors"},{"key":"1380_CR46","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1186\/s13071-018-3198-5","volume":"11","author":"HN Ren","year":"2018","unstructured":"Ren HN, Guo KX, Zhang Y, Sun GG, Liu RD, Jiang P, Zhang X, Wang L, Cui J, Wang ZQ (2018) Molecular characterization of a 31 kDa protein from Trichinella spiralis and its induced immune protection in BALB\/c mice. Parasit Vectors 11:625","journal-title":"Parasit Vectors"},{"key":"1380_CR47","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.rvsc.2020.03.012","volume":"130","author":"Y Han","year":"2020","unstructured":"Han Y, Yue X, Hu CX, Liu F, Liu RD, He MM, Long SR, Cui J, Wang ZQ (2020) Interaction of a Trichinella spiralis cathepsin B with enterocytes promotes the larval intrusion into the cells. Res Vet Sci 130:110\u2013117","journal-title":"Res Vet Sci"},{"key":"1380_CR48","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/s13567-020-00838-1","volume":"51","author":"JJ Lei","year":"2020","unstructured":"Lei JJ, Hu YY, Liu F, Yan SW, Liu RD, Long SR, Jiang P, Cui J, Wang ZQ (2020) Molecular cloning and characterization of a novel peptidase from Trichinella spiralis and protective immunity elicited by the peptidase in BALB\/c mice. Vet Res 51:111","journal-title":"Vet Res"},{"key":"1380_CR49","doi-asserted-by":"publisher","first-page":"e0011816","DOI":"10.1371\/journal.pntd.0011816","volume":"17","author":"RD Liu","year":"2023","unstructured":"Liu RD, Meng XY, Li CL, Lin XZ, Xu QY, Xu H, Long SR, Cui J, Wang ZQ (2023) Trichinella spiralis cathepsin L damages the tight junctions of intestinal epithelial cells and mediates larval invasion. PLoS Negl Trop Dis 17:e0011816","journal-title":"PLoS Negl Trop Dis"},{"key":"1380_CR50","doi-asserted-by":"publisher","first-page":"e0011872","DOI":"10.1371\/journal.pntd.0011872","volume":"18","author":"YY Song","year":"2024","unstructured":"Song YY, Zhang XZ, Wang BN, Cheng YK, Guo X, Zhang X, Long SR, Liu RD, Wang ZQ, Cui J (2024) A novel Trichinella spiralis serine proteinase disrupted gut epithelial barrier and mediated larval invasion through binding to RACK1 and activating MAPK\/ERK1\/2 pathway. PLoS Negl Trop Dis 18:e0011872","journal-title":"PLoS Negl Trop Dis"},{"key":"1380_CR51","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1586\/14787210.1.3.471","volume":"1","author":"E Pozio","year":"2003","unstructured":"Pozio E, Gomez Morales MA, Dupouy-Camet J (2003) Clinical aspects, diagnosis and treatment of trichinellosis. Expert Rev Anti Infect Ther 1:471\u2013482","journal-title":"Expert Rev Anti Infect Ther"},{"key":"1380_CR52","doi-asserted-by":"publisher","first-page":"179","DOI":"10.2478\/helm-2021-0016","volume":"58","author":"AM Fahmy","year":"2021","unstructured":"Fahmy AM, Diab TM (2021) Therapeutic efficacy of Albendazole and Mefloquine alone or in combination against early and late stages of Trichinella spiralis infection in mice. Helminthologia 58:179\u2013187","journal-title":"Helminthologia"},{"key":"1380_CR53","doi-asserted-by":"publisher","first-page":"2331","DOI":"10.1007\/s00436-016-4982-9","volume":"115","author":"AE Saad","year":"2016","unstructured":"Saad AE, Ashour DS, Abou Rayia DM, Bedeer AE (2016) Carbonic anhydrase enzyme as a potential therapeutic target for experimental trichinellosis. Parasitol Res 115:2331\u20132339","journal-title":"Parasitol Res"},{"key":"1380_CR54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-3-031-00793-4_1","volume":"99","author":"AN G\u00fcnenc","year":"2022","unstructured":"G\u00fcnenc AN, Graf B, Stark H, Chari A (2022) Fatty acid synthase: structure, function, and regulation. Subcell Biochem 99:1\u201333","journal-title":"Subcell Biochem"},{"key":"1380_CR55","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/s13071-024-06213-9","volume":"17","author":"TY He","year":"2024","unstructured":"He TY, Li YT, Liu ZD, Cheng H, Bao YF, Zhang JL (2024) Lipid metabolism: the potential targets for toxoplasmosis treatment. Parasit Vectors 17:111","journal-title":"Parasit Vectors"},{"key":"1380_CR56","doi-asserted-by":"publisher","first-page":"1561","DOI":"10.1016\/j.ijpara.2009.06.005","volume":"39","author":"KC Fairfax","year":"2009","unstructured":"Fairfax KC, Vermeire JJ, Harrison LM, Bungiro RD, Grant W, Husain SZ, Cappello M (2009) Characterisation of a fatty acid and retinol binding protein orthologue from the hookworm Ancylostoma ceylanicum. Int J Parasitol 39:1561\u20131571","journal-title":"Int J Parasitol"},{"key":"1380_CR57","doi-asserted-by":"publisher","first-page":"12612","DOI":"10.1074\/jbc.275.17.12612","volume":"275","author":"G Martin","year":"2000","unstructured":"Martin G, Poirier H, Crombie D, Fruchart JC, Heyman RA, Besnard P, Auwerx J, Hennuyer N (2000) Induction of the fatty acid transport protein 1 and acyl-CoA synthase genes by dimer-selective rexinoids suggests that the peroxisome proliferator-activated receptor-retinoid X receptor heterodimer is their molecular target. J Biol Chem 275:12612\u201312618","journal-title":"J Biol Chem"},{"key":"1380_CR58","doi-asserted-by":"publisher","first-page":"e0006485","DOI":"10.1371\/journal.pntd.0006485","volume":"12","author":"GG Sun","year":"2018","unstructured":"Sun GG, Song YY, Jiang P, Ren HN, Yan SW, Han Y, Liu RD, Zhang X, Wang ZQ, Cui J (2018) Characterization of a Trichinella spiralis putative serine protease. Study of its potential as sero-diagnostic tool. PLoS Negl Trop Dis 12:e0006485","journal-title":"PLoS Negl Trop Dis"},{"key":"1380_CR59","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.1016\/j.bbrc.2018.06.109","volume":"503","author":"J Li","year":"2018","unstructured":"Li J, Xiang M, Zhang R, Xu B, Hu W (2018) RNA interference in vivo in Schistosoma japonicum: Establishing and optimization of RNAi mediated suppression of gene expression by long dsRNA in the intra-mammalian life stages of worms. Biochem Biophys Res Commun 503:1004\u20131010","journal-title":"Biochem Biophys Res Commun"},{"key":"1380_CR60","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/S0166-6851(03)00092-6","volume":"129","author":"AA Aboobaker","year":"2003","unstructured":"Aboobaker AA, Blaxter ML (2003) Use of RNA interference to investigate gene function in the human filarial nematode parasite Brugia malayi. Mol Biochem Parasitol 129:41\u201351","journal-title":"Mol Biochem Parasitol"},{"key":"1380_CR61","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1128\/EC.00255-07","volume":"6","author":"J Mazumdar","year":"2007","unstructured":"Mazumdar J, Striepen B (2007) Make it or take it: fatty acid metabolism of apicomplexan parasites. Eukaryot Cell 6:1727\u20131735","journal-title":"Eukaryot Cell"},{"key":"1380_CR62","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1186\/s13567-020-00847-0","volume":"51","author":"X Yue","year":"2020","unstructured":"Yue X, Sun XY, Liu F, Hu CX, Bai Y, Da Yang Q, Liu RD, Zhang X, Cui J, Wang ZQ (2020) Molecular characterization of a Trichinella spiralis serine proteinase. Vet Res 51:125","journal-title":"Vet Res"},{"key":"1380_CR63","doi-asserted-by":"publisher","first-page":"e1330246","DOI":"10.1080\/21624054.2017.1330246","volume":"6","author":"V La\u017eeti\u0107","year":"2017","unstructured":"La\u017eeti\u0107 V, Fay DS (2017) Molting in C. elegans. Worm 6:e1330246","journal-title":"Worm"},{"key":"1380_CR64","doi-asserted-by":"publisher","first-page":"1853","DOI":"10.1128\/IAI.70.4.1853-1859.2002","volume":"70","author":"LF Gagliardo","year":"2002","unstructured":"Gagliardo LF, McVay CS, Appleton JA (2002) Molting, ecdysis, and reproduction of Trichinella spiralis are supported in vitro by intestinal epithelial cells. Infect Immun 70:1853\u20131859","journal-title":"Infect Immun"}],"container-title":["Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-024-01380-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13567-024-01380-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-024-01380-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T13:08:18Z","timestamp":1727701698000},"score":1,"resource":{"primary":{"URL":"https:\/\/veterinaryresearch.biomedcentral.com\/articles\/10.1186\/s13567-024-01380-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,30]]},"references-count":64,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["1380"],"URL":"https:\/\/doi.org\/10.1186\/s13567-024-01380-0","relation":{},"ISSN":["1297-9716"],"issn-type":[{"value":"1297-9716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,30]]},"assertion":[{"value":"27 May 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 July 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 September 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experimental animals are raised and cared based on the National Guidelines for Experimental Animal Welfare of the People\u2019s Republic of China (2006). Ethical approval was acquired from the Life Science Ethics Committee of Zhengzhou University (No. ZZUIRB GZR 2021-0044).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"126"}}