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Here, we report on the frequency and distribution of culturable bacteria collected from\n                    <jats:italic>Stomoxys<\/jats:italic>\n                    flies captured in free stall barns and nearby calf hutches over a three-month period on a focal research farm in Wisconsin, USA. Mastitis-associated bacterial taxa, including\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    , and\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    spp., were frequently isolated from pooled samples of the internal or external portions of the flies. Conversely, selective enrichment protocols from these samples yielded only a single isolate of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    and no enterohemorrhagic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    O157. Neither trap location nor time of capture had a significant impact on the observed frequency of most bacterial genera isolated from the flies. Our results confirm that\n                    <jats:italic>Stomoxys<\/jats:italic>\n                    flies harbor both mastitis-associated bacterial taxa and bacterial taxa associated with opportunistic infections in humans. Further research into the transmission of fly-associated microbes could be important in the control of mastitis or other bacterial diseases on dairy farms.\n                  <\/jats:p>","DOI":"10.1186\/s13567-025-01458-3","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T11:42:07Z","timestamp":1739274127000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Opportunistic pathogens are prevalent across the culturable exogenous and endogenous microbiota of stable flies captured at a dairy facility"],"prefix":"10.1186","volume":"56","author":[{"given":"Andrew J.","family":"Sommer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Courtney L.","family":"Deblois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew D. 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