{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:45:31Z","timestamp":1787701531415,"version":"build-2784847793"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T00:00:00Z","timestamp":1742601600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T00:00:00Z","timestamp":1742601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102694"],"award-info":[{"award-number":["32102694"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The apicomplexan parasite\n                    <jats:italic>Eimeria<\/jats:italic>\n                    is the causative agent of coccidiosis, which results in enteritis in animals and imposes a significant economic burden on the farming industry. Apicomplexan\u00a0Apetala2\/ERF (ApiAP2) transcription factors are known to play crucial roles in various processes of Apicomplexa parasites. However, little is understood regarding the role of ApiAP2s in\n                    <jats:italic>Eimeria<\/jats:italic>\n                    species. In this study, we performed a functional characterisation of ApiAP2 through CRISPR-Cas9-mediated knockout. Our results revealed that the parasite\u2019s invasion efficiency, total oocyst output, and virulence were significantly impaired after EtAP2-S1 depletion. RNA sequencing (RNA-Seq) and Cleavage Under Targets and Tagmentation (CUT&amp;Tag) analyses showed that EtAP2-S1 targets the promoters of numerous genes, and its knockout resulted in the up-regulation of 59\n                    <jats:italic>sag<\/jats:italic>\n                    genes. Furthermore, the knockout strain exhibited significantly lower virulence while offering excellent immune protection, making it a promising candidate for a vaccine. This study demonstrates that EtAP2-S1 is a fitness-conferring gene that suppresses the expression of\n                    <jats:italic>sag<\/jats:italic>\n                    genes in\n                    <jats:italic>E. tenella<\/jats:italic>\n                    , which is the first step in developing gene-knockout vaccines to control coccidiosis.\n                  <\/jats:p>","DOI":"10.1186\/s13567-025-01491-2","type":"journal-article","created":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T00:38:25Z","timestamp":1742603905000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular mechanisms of surface antigen suppression by ApiAP2 and its implications for vaccine development"],"prefix":"10.1186","volume":"56","author":[{"given":"Peiyao","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rina","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganglin","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Si","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingju","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9917-4817","authenticated-orcid":false,"given":"Dandan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,22]]},"reference":[{"key":"1491_CR1","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/s44280-024-00039-x","volume":"2","author":"Y Gao","year":"2024","unstructured":"Gao Y, Sun P, Hu D, Tang X, Zhang S, Shi F, Yan X, Yan W, Shi T, Wang S, Zou J, Yin G, Liu X, Dong H, Suo X (2024) Advancements in understanding chicken coccidiosis: from Eimeria biology to innovative control strategies. One Health Adv 2:6","journal-title":"One Health Adv"},{"key":"1491_CR2","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1186\/s13567-020-00837-2","volume":"51","author":"DP Blake","year":"2020","unstructured":"Blake DP, Knox J, Dehaeck B, Huntington B, Rathinam T, Ravipati V, Ayoade S, Gilbert W, Adebambo AO, Jatau ID, Raman M, Parker D, Rushton J, Tomley FM (2020) Re-calculating the cost of coccidiosis in chickens. Vet Res 51:115","journal-title":"Vet Res"},{"key":"1491_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1201\/9780429294105","volume-title":"Biology of intestinal coccidia, Coccidiosis in livestock, poultry, companion animals, and humans","author":"J Dubey","year":"2019","unstructured":"Dubey J, Lindsay D, Jenkins M, Bauer C (2019) Biology of intestinal coccidia, Coccidiosis in livestock, poultry, companion animals, and humans. CRC Press, Boca Raton, pp 1\u201336"},{"key":"1491_CR4","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.787653","volume":"8","author":"C Mesa-Pineda","year":"2021","unstructured":"Mesa-Pineda C, Navarro-Ru\u00edz JL, L\u00f3pez-Osorio S, Chaparro-Guti\u00e9rrez JJ, G\u00f3mez-Osorio LM (2021) Chicken coccidiosis: from the parasite lifecycle to control of the disease. Front Vet Sci 8:787653","journal-title":"Front Vet Sci"},{"key":"1491_CR5","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1017\/S0031182019001562","volume":"147","author":"A Burrell","year":"2020","unstructured":"Burrell A, Tomley FM, Vaughan S, Marugan-Hernandez V (2020) Life cycle stages, specific organelles and invasion mechanisms of Eimeria species. Parasitology 147:263\u2013278","journal-title":"Parasitology"},{"key":"1491_CR6","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1111\/mmi.14634","volume":"115","author":"L Augusto","year":"2021","unstructured":"Augusto L, Wek RC, Sullivan WJ Jr (2021) Host sensing and signal transduction during Toxoplasma stage conversion. Mol Microbiol 115:839\u2013848","journal-title":"Mol Microbiol"},{"key":"1491_CR7","doi-asserted-by":"publisher","first-page":"831","DOI":"10.3390\/genes15070831","volume":"15","author":"N Zhang","year":"2024","unstructured":"Zhang N, Li X, Liu J, Chen L, Zhang S, Liu X, Tang X, Suo X, Zhang Y (2024) Transcriptomic insights into the developmental dynamics of Eimeria acervulina: a comparative study of a precocious line and the wild type. Genes 15:831","journal-title":"Genes"},{"key":"1491_CR8","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1186\/s13071-023-05828-8","volume":"16","author":"L Chen","year":"2023","unstructured":"Chen L, Tang X, Sun P, Hu D, Zhang Y, Wang C, Chen J, Liu J, Gao Y, Hao Z, Zhang N, Chen W, Xie F, Suo X, Liu X (2023) Comparative transcriptome profiling of Eimeria tenella in various developmental stages and functional analysis of an ApiAP2 transcription factor exclusively expressed during sporogony. Parasit Vectors 16:241","journal-title":"Parasit Vectors"},{"key":"1491_CR9","doi-asserted-by":"publisher","first-page":"47","DOI":"10.3390\/pathogens8020047","volume":"8","author":"MD Jeninga","year":"2019","unstructured":"Jeninga MD, Quinn JE, Petter M (2019) ApiAP2 transcription factors in apicomplexan parasites. Pathogens 8:47","journal-title":"Pathogens"},{"key":"1491_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.molbiopara.2010.11.014","volume":"176","author":"HJ Painter","year":"2011","unstructured":"Painter HJ, Campbell TL, Llin\u00e1s M (2011) The Apicomplexan AP2 family: integral factors regulating Plasmodium development. Mol Biochem Parasitol 176:1\u20137","journal-title":"Mol Biochem Parasitol"},{"key":"1491_CR11","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1111\/mmi.12121","volume":"87","author":"R Walker","year":"2013","unstructured":"Walker R, Gissot M, Croken MM, Huot L, Hot D, Kim K, Tomavo S (2013) The Toxoplasma nuclear factor TgAP2XI-4 controls bradyzoite gene expression and cyst formation. Mol Microbiol 87:641\u2013655","journal-title":"Mol Microbiol"},{"key":"1491_CR12","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/mSphere.00054-17","volume":"2","author":"S Huang","year":"2017","unstructured":"Huang S, Holmes MJ, Radke JB, Hong D-P, Liu T-K, White MW, Sullivan WJ Jr (2017) Toxoplasma gondii AP2IX-4 regulates gene expression during bradyzoite development. mSphere. 2:10\u20131128","journal-title":"mSphere."},{"key":"1491_CR13","doi-asserted-by":"publisher","first-page":"e00347","DOI":"10.1128\/mSphere.00347-16","volume":"2","author":"D-P Hong","year":"2017","unstructured":"Hong D-P, Radke JB, White MW (2017) Opposing transcriptional mechanisms regulate Toxoplasma development. mSphere 2:e00347-16","journal-title":"mSphere"},{"key":"1491_CR14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007035","volume":"14","author":"JB Radke","year":"2018","unstructured":"Radke JB, Worth D, Hong D, Huang S, Sullivan WJ Jr, Wilson EH, White MW (2018) Transcriptional repression by ApiAP2 factors is central to chronic toxoplasmosis. PLoS Pathog 14:e1007035","journal-title":"PLoS Pathog"},{"key":"1491_CR15","doi-asserted-by":"publisher","first-page":"6871","DOI":"10.1073\/pnas.1300059110","volume":"110","author":"JB Radke","year":"2013","unstructured":"Radke JB, Lucas O, De Silva EK, Ma Y, Sullivan WJ Jr, Weiss LM, Llinas M, White MW (2013) ApiAP2 transcription factor restricts development of the Toxoplasma tissue cyst. Proc Natl Acad Sci USA 110:6871\u20136876","journal-title":"Proc Natl Acad Sci USA"},{"key":"1491_CR16","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/j.cell.2019.12.013","volume":"182","author":"BS Waldman","year":"2020","unstructured":"Waldman BS, Schwarz D, Wadsworth MH II, Saeij JP, Shalek AK, Lourido S (2020) Identification of a master regulator of differentiation in Toxoplasma. Cell 182:359\u2013372","journal-title":"Cell"},{"key":"1491_CR17","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1038\/s41564-023-01358-2","volume":"8","author":"MH Licon","year":"2023","unstructured":"Licon MH, Giuliano CJ, Chan AW, Chakladar S, Eberhard JN, Shallberg LA, Chandrasekaran S, Waldman BS, Koshy AA, Hunter CA, Lourido S (2023) A positive feedback loop controls Toxoplasma chronic differentiation. Nat Microbiol 8:889\u2013904","journal-title":"Nat Microbiol"},{"key":"1491_CR18","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/nature12970","volume":"507","author":"A Sinha","year":"2014","unstructured":"Sinha A, Hughes KR, Modrzynska KK, Otto TD, Pfander C, Dickens NJ, Religa AA, Bushell E, Graham AL, Cameron R, Kafsack BFC, Williams AE, Llinas M, Berriman M, Billker O, Waters AP (2014) A cascade of DNA-binding proteins for sexual commitment and development in Plasmodium. Nature 507:253\u2013257","journal-title":"Nature"},{"key":"1491_CR19","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1038\/nature12920","volume":"507","author":"BF Kafsack","year":"2014","unstructured":"Kafsack BF, Rovira-Graells N, Clark TG, Bancells C, Crowley VM, Campino SG, Williams AE, Drought LG, Kwiatkowski DP, Baker DA, Cort\u00e9s A, Llin\u00e1s M (2014) A transcriptional switch underlies commitment to sexual development in malaria parasites. Nature 507:248\u2013252","journal-title":"Nature"},{"key":"1491_CR20","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.chom.2014.07.004","volume":"16","author":"NM Brancucci","year":"2014","unstructured":"Brancucci NM, Bertschi NL, Zhu L, Niederwieser I, Chin WH, Wampfler R, Freymond C, Rottmann M, Felger I, Bozdech Z, Voss TS (2014) Heterochromatin protein 1 secures survival and transmission of malaria parasites. Cell Host Microbe 16:165","journal-title":"Cell Host Microbe"},{"key":"1491_CR21","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1038\/s41564-020-0674-4","volume":"5","author":"DC Farhat","year":"2020","unstructured":"Farhat DC, Swale C, Dard C, Cannella D, Ortet P, Barakat M, Sindikubwabo F, Belmudes L, De Bock P-J, Cout\u00e9 Y, Bougdour A, Hakimi M-A (2020) A MORC-driven transcriptional switch controls Toxoplasma developmental trajectories and sexual commitment. Nat Microbiol 5:570\u2013583","journal-title":"Nat Microbiol"},{"key":"1491_CR22","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1038\/s41586-023-06821-y","volume":"625","author":"AV Antunes","year":"2024","unstructured":"Antunes AV, Shahinas M, Swale C, Farhat DC, Ramakrishnan C, Bruley C, Cannella D, Robert MG, Corrao C, Cout\u00e9 Y, Hehl AB, Bougdour A, Coppens I, Hakimi M-A (2024) In vitro production of cat-restricted Toxoplasma pre-sexual stages. Nature 625:366\u2013376","journal-title":"Nature"},{"key":"1491_CR23","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1038\/s41467-024-44967-z","volume":"15","author":"J Wang","year":"2024","unstructured":"Wang J, Li T, Zhang N, Wang M, Sun L, Zhang Z, Fu B, Elsheikha HM, Zhu X (2024) The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony. Nat Commun 15:793","journal-title":"Nat Commun"},{"key":"1491_CR24","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01785-23","volume":"14","author":"F Fan","year":"2023","unstructured":"Fan F, Xue L, Yin X, Gupta N, Shen B (2023) AP2XII-1 is a negative regulator of merogony and presexual commitment in Toxoplasma gondii. MBio 14:e0178523","journal-title":"MBio"},{"key":"1491_CR25","doi-asserted-by":"publisher","first-page":"5527","DOI":"10.3390\/ijms25105527","volume":"25","author":"Y Jiang","year":"2024","unstructured":"Jiang Y, Shi Y, Xue Y, Hu D, Song X (2024) AP2XII-1 and AP2XI-2 suppress schizogony gene expression in Toxoplasma gondii. Int J Mol Sci 25:5527","journal-title":"Int J Mol Sci"},{"key":"1491_CR26","doi-asserted-by":"publisher","first-page":"128","DOI":"10.3389\/fbioe.2020.00128","volume":"8","author":"D Hu","year":"2020","unstructured":"Hu D, Tang X, Ben Mamoun C, Wang C, Wang S, Gu X, Duan C, Zhang S, Suo J, Deng M, Yu Y, Suo X, Liu X (2020) Efficient single-gene and gene family editing in the Apicomplexan parasite Eimeria tenella using CRISPR-Cas9. Front Bioeng Biotechnol 8:128","journal-title":"Front Bioeng Biotechnol"},{"key":"1491_CR27","unstructured":"Eckert J, Braun R, Shirley MW, Coudert P. Biotechnology: guidelines on techniques in coccidiosis research. Luxembourg: European commission directorate-general XII, science, research and development; 1995."},{"key":"1491_CR28","doi-asserted-by":"publisher","DOI":"10.3791\/60552","author":"C Duan","year":"2020","unstructured":"Duan C, Tang X, Hu D, Zhang S, Liu J, Bi F, Hao Z, Suo J, Yu Y, Wang M, Sun P, Du L, Suo X, Liu X (2020) Nucleofection and in vivo propagation of chicken Eimeria parasites. J Vis Exp 156:e60552","journal-title":"J Vis Exp"},{"key":"1491_CR29","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/0014-4894(70)90063-9","volume":"28","author":"J Johnson","year":"1970","unstructured":"Johnson J, Reid WM (1970) Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol 28:30\u201336","journal-title":"Exp Parasitol"},{"key":"1491_CR30","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2023.103234","volume":"103","author":"L Zhang","year":"2024","unstructured":"Zhang L, Song Z, Li P, Song X, Tang X, Hu D (2024) Molecular characterization and immune protective efficacy of 3 Eimeria tenella antigens. Poult Sci 103:103234","journal-title":"Poult Sci"},{"key":"1491_CR31","doi-asserted-by":"publisher","first-page":"225","DOI":"10.12688\/wellcomeopenres.17100.1","volume":"6","author":"E Aunin","year":"2021","unstructured":"Aunin E, B\u00f6hme U, Blake D, Dove A, Smith M, Corton C, Oliver K, Betteridge E, Quail MA, McCarthy SA, Wood J, Tracey A, Torrance J, Sims Y, Howe K, Challis R, Berriman M, Reid A (2021) The complete genome sequence of Eimeria tenella (Tyzzer 1929), a common gut parasite of chickens. Wellcome Open Res 6:225","journal-title":"Wellcome Open Res"},{"key":"1491_CR32","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","volume":"37","author":"D Kim","year":"2019","unstructured":"Kim D, Paggi JM, Park C, Bennett C, Salzberg SL (2019) Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype. Nat Biotechnol 37:907\u2013915","journal-title":"Nat Biotechnol"},{"key":"1491_CR33","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R, Subgroup GPDP (2009) The sequence alignment\/map format and SAMtools. Bioinformatics 25:2078\u20132079","journal-title":"Bioinformatics"},{"key":"1491_CR34","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","volume":"31","author":"S Anders","year":"2015","unstructured":"Anders S, Pyl PT, Huber W (2015) HTSeq\u2014a Python framework to work with high-throughput sequencing data. Bioinformatics 31:166\u2013169","journal-title":"Bioinformatics"},{"key":"1491_CR35","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15:550","journal-title":"Genome Biol"},{"key":"1491_CR36","doi-asserted-by":"crossref","unstructured":"Wu T, Hu E, Xu S, Chen M, Guo P, Dai Z, Feng T, Zhou L, Tang W, Zhan L, Fu X, Liu S, Bo X, Yu G (2021) ClusterProfiler 4.0: A universal enrichment tool for interpreting omics data. Innovation 2:100141","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"1491_CR37","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.1923","volume":"9","author":"B Langmead","year":"2012","unstructured":"Langmead B, Salzberg SL (2012) Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357\u2013359","journal-title":"Nat Methods"},{"key":"1491_CR38","doi-asserted-by":"crossref","unstructured":"Gaspar JM (2018) Improved peak-calling with MACS2. BioRxiv:496521","DOI":"10.1101\/496521"},{"key":"1491_CR39","doi-asserted-by":"publisher","first-page":"1884","DOI":"10.1002\/sim.9334","volume":"41","author":"R Singh","year":"2022","unstructured":"Singh R, Zhang F, Li Q (2022) Assessing reproducibility of high-throughput experiments in the case of missing data. Statist Med 41:1884\u20131899","journal-title":"Statist Med"},{"key":"1491_CR40","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1093\/bib\/bbs017","volume":"14","author":"H Thorvaldsd\u00f3ttir","year":"2013","unstructured":"Thorvaldsd\u00f3ttir H, Robinson JT, Mesirov JP (2013) Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration. Brief Bioinform 14:178\u2013192","journal-title":"Brief Bioinform"},{"key":"1491_CR41","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1186\/s12864-018-5090-2","volume":"19","author":"D Hu","year":"2018","unstructured":"Hu D, Wang C, Wang S, Tang X, Duan C, Zhang S, Suo J, Deng M, Lv Y, Suo X (2018) Comparative transcriptome analysis of Eimeria maxima (Apicomplexa: Eimeriidae) suggests DNA replication activities correlating with its fecundity. BMC Genomics 19:699","journal-title":"BMC Genomics"},{"key":"1491_CR42","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.3390\/life13061295","volume":"13","author":"JD Britez","year":"2023","unstructured":"Britez JD, Rodriguez AE, Di Ciaccio L, Marug\u00e1n-Hernandez V, Tomazic ML (2023) What do we know about surface proteins of chicken parasites Eimeria? Life 13:1295","journal-title":"Life"},{"key":"1491_CR43","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.1101\/gr.168955.113","volume":"24","author":"AJ Reid","year":"2014","unstructured":"Reid AJ, Blake DP, Ansari HR, Billington K, Browne HP, Bryant J, Dunn M, Hung SS, Kawahara F, Mirandasaavedra D, Malas TB, Mourier T, Naghra H, Nair M, Otto TD, Rawlings ND, Rivailler P, Sanchez-Flores A, Sanders M, Subramaniam C, Tay Y-L, Woo Y, Wu X, Barrell B, Dear PH, Doerig C, Gruber A, Ivens AC, Parkinson J, Rajandream M-A, Shirley MW, Wan K-L, Berriman M, Tomley FM, Pain A (2014) Genomic analysis of the causative agents of coccidiosis in domestic chickens. Genome Res 24:1676\u20131685","journal-title":"Genome Res"},{"key":"1491_CR44","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.54129","volume":"9","author":"Y Xue","year":"2020","unstructured":"Xue Y, Theisen T, Rastogi S, Ferrel A, Quake S, Boothroyd J (2020) A single-parasite transcriptional atlas of Toxoplasma gondii reveals novel control of antigen expression. Elife 9:e54129","journal-title":"Elife"},{"key":"1491_CR45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006379","volume":"13","author":"S Long","year":"2017","unstructured":"Long S, Brown KM, Drewry LL, Anthony B, Phan IQ, Sibley LD (2017) Calmodulin-like proteins localized to the conoid regulate motility and cell invasion by Toxoplasma gondii. PLoS Pathog 13:e1006379","journal-title":"PLoS Pathog"},{"key":"1491_CR46","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1111\/j.1365-2958.2004.04464.x","volume":"55","author":"S Luo","year":"2005","unstructured":"Luo S, Ruiz FA, Moreno SN (2005) The acidocalcisome Ca2+-ATPase (TgA1) of Toxoplasma gondii is required for polyphosphate storage, intracellular calcium homeostasis and virulence. Mol Microbiol 55:1034\u20131045","journal-title":"Mol Microbiol"},{"key":"1491_CR47","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.pt.2013.10.003","volume":"30","author":"DP Blake","year":"2014","unstructured":"Blake DP, Tomley FM (2014) Securing poultry production from the ever-present Eimeria challenge. Trends Parasitol 30:12\u201319","journal-title":"Trends Parasitol"},{"key":"1491_CR48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001279","volume":"7","author":"DP Blake","year":"2011","unstructured":"Blake DP, Billington KJ, Copestake SL, Oakes RD, Quail MA, Wan K-L, Shirley MW, Smith AL (2011) Genetic mapping identifies novel highly protective antigens for an apicomplexan parasite. PLoS Pathog 7:e1001279","journal-title":"PLoS Pathog"},{"key":"1491_CR49","doi-asserted-by":"publisher","first-page":"E5343","DOI":"10.1073\/pnas.1506468112","volume":"112","author":"DP Blake","year":"2015","unstructured":"Blake DP, Clark EL, Macdonald SE, Thenmozhi V, Kundu K, Garg R, Jatau ID, Ayoade S, Kawahara F, Moftah A, Reid AJ, Adebambo AO, Zapata R\u00c1, Rao ASRS, Thangaraj K, Banerjee PS, Dhinakar-Raj G, Raman M, Tomley FM (2015) Population, genetic, and antigenic diversity of the apicomplexan Eimeria tenella and their relevance to vaccine development. Proc Natl Acad Sci USA 112:E5343\u2013E5350","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-025-01491-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13567-025-01491-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-025-01491-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T20:53:21Z","timestamp":1742849601000},"score":1,"resource":{"primary":{"URL":"https:\/\/veterinaryresearch.biomedcentral.com\/articles\/10.1186\/s13567-025-01491-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,22]]},"references-count":49,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["1491"],"URL":"https:\/\/doi.org\/10.1186\/s13567-025-01491-2","relation":{},"ISSN":["1297-9716"],"issn-type":[{"value":"1297-9716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,22]]},"assertion":[{"value":"7 December 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 January 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments in this study were approved by the Administration Committee of Laboratory Animals at Guangxi University and were conducted in accordance with the Institutional Animal Care and Use Committee guidelines (Approval number: GXU-2022-265).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"63"}}