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Here, we describe a new lymph node infection model where 7\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u00a0CFU <jats:italic>S<\/jats:italic>. <jats:italic>suis<\/jats:italic> serotype 2 were injected in the left cervical lymph node. In total, seven out of eight 5-week-old piglets developed clinical signs of disease. In all infected pigs, <jats:italic>S<\/jats:italic>. <jats:italic>suis<\/jats:italic> was reisolated from the infected lymph node and one or more additional sites. Immunohistochemically, <jats:italic>S<\/jats:italic>. <jats:italic>suis<\/jats:italic> antigen was located predominantly in the sinus. The results suggest that <jats:italic>S<\/jats:italic>. <jats:italic>suis<\/jats:italic> easily disseminates after lymph node infection and that the interaction with cells of the mononuclear phagocytic system, primarily in the lymph node sinus, plays an important role in host cell\u2013pathogen interaction.<\/jats:p>","DOI":"10.1186\/s13567-025-01616-7","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T08:11:04Z","timestamp":1759392664000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A new lymph node infection model for Streptococcus suis serotype 2 in pigs"],"prefix":"10.1186","volume":"56","author":[{"given":"Annika Katharina","family":"Breitfelder","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Sattler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Kauffold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Pfetzing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Majcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiner","family":"Ulrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"\u00d6hlmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karoline","family":"Rieckmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph Georg","family":"Baums","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"1616_CR1","doi-asserted-by":"publisher","first-page":"934","DOI":"10.1002\/9781119350927.ch61","volume-title":"Disease of swine","author":"M Gottschalk","year":"2019","unstructured":"Gottschalk M, Segura M (2019) Streptococcosis. 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The animal experiment (permit number TVV 03\/24, file number 25-5131\/604\/4) as well as killing of one additional pig for research purposes (permit number TVT 5\/2023, file number 25-5131\/554\/6) were approved by the \u201cLandesdirektion Sachsen\u201d, which includes review through the registered ethics committee of this institution.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare\u00a0that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"186"}}