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Ante mortem diagnosis of TB is hindered by the limited sensitivity of available techniques and false-positive results from other mycobacterial infections, such as paratuberculosis (PTB). Nuclear Magnetic Resonance (NMR)-based metabolomics provides unique fingerprinting of the disease\u2019s metabolic status, making it a promising diagnostic tool. However, conventional high-resolution NMR has limitations in veterinary practice, where high costs and large equipment size are major constraints. Benchtop NMR spectrometer is proposed as a compact, cost-effective alternative for livestock farms. The study aimed to evaluate NMR-based metabolomics as a diagnostic tool and transfer it from high-resolution to benchtop NMR spectrometers in an animal setting. Serum samples from TB-infected, PTB-infected (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200916), and healthy control goats (HC) were analyzed by both high-resolution and benchtop NMR spectroscopy. Multivariate statistical analysis successfully differentiated groups on the basis of their metabolic profiles with both spectrometers. We identified that betaine, glucose, glycerol, and lactate are significantly capable of distinguishing between the three groups. Additionally, 3-hydroxybutyrate, creatine, glutamate, leucine, lysine, phenylalanine, threonine, and tyrosine further differentiate TB from HC. Acetate, creatine, glutamate, isoleucine, leucine, and lysine distinguish TB from PTB, while 3-hydroxybutyrate and phenylalanine serve to differentiate PTB from HC. Analyses with both high-resolution and benchtop spectrometers demonstrated high sensitivity and accuracy and reliable metabolite identification. These findings highlight NMR\u2019s spectroscopy potential to identify biomarkers associated with TB and PTB infection, improving health management in livestock.\n                  <\/jats:p>","DOI":"10.1186\/s13567-025-01686-7","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T11:15:50Z","timestamp":1765538150000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metabolomic signatures of tuberculosis and paratuberculosis in goats revealed by high-resolution and benchtop NMR spectroscopy"],"prefix":"10.1186","volume":"57","author":[{"given":"Pilar","family":"Alonso-Moreno","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Ortiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Ortega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Velasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Bezos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Luis","family":"Izquierdo-Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"1686_CR1","doi-asserted-by":"publisher","first-page":"S44","DOI":"10.1016\/j.rvsc.2014.04.002","volume":"97","author":"J Bezos","year":"2014","unstructured":"Bezos J, Casal C, Romero B, Schroeder B, Hardegger R, Raeber AJ, L\u00f3pez L, Rueda P, Dom\u00ednguez L (2014) Current ante-mortem techniques for diagnosis of bovine tuberculosis. 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