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Only the former is zoonotic and of public health concern, but both cause significant economic losses, thus requiring effective vaccines. The sole vaccine available,\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    Rev1, protects against both infections but induces antibodies detected by S-LPS-based tests used for\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    surveillance.  Since controlling this zoonotic species is a priority, Rev1 is banned in\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    -free areas. Consequently,\n                    <jats:italic>B. ovis<\/jats:italic>\n                    (detected by R-LPS-based tests) remains endemic or re-emerges, and an R brucellosis vaccine is needed. Previously, we demonstrated that subcutaneous vaccination with\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    R mutant H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    protects rams against\n                    <jats:italic>B. ovis<\/jats:italic>\n                    similarly to Rev1, without interfering in the official S-LPS-based Rose Bengal and Complement Fixation tests. However, H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    elicits R-LPS antibodies that interfere with\n                    <jats:italic>B. ovis<\/jats:italic>\n                    tests. Here, we explored two strategies to minimize this interference: (i) altering H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    relevant diagnostic epitopes by constructing\n                    <jats:italic>wadB<\/jats:italic>\n                    and\n                    <jats:italic>wadC<\/jats:italic>\n                    mutants deleted in sugars of the R-LPS core tetrasaccharide branch; and (ii) administering H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    conjunctivally, a route known to reduce antibody responses to S brucellosis vaccines. While the core-defective mutants were over-attenuated and failed to protect mice, the conjunctival route preserved H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    efficacy in rams and significantly reduced serological interference. Conjunctival H38\u0394\n                    <jats:italic>wbkF<\/jats:italic>\n                    vaccine is thus a suitable tool for\n                    <jats:italic>B. ovis<\/jats:italic>\n                    eradication in\n                    <jats:italic>B. melitensis<\/jats:italic>\n                    -free areas.\n                  <\/jats:p>","DOI":"10.1186\/s13567-025-01693-8","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T03:33:58Z","timestamp":1772940838000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Conjunctival administration of H38\u0394wbkF rough vaccine as an effective strategy to protect against Brucella ovis infection while minimizing serological interference"],"prefix":"10.1186","volume":"57","author":[{"given":"Nerea","family":"Lopez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Andr\u00e9s-Barranco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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