{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:40:09Z","timestamp":1787496009295,"version":"build-2736575974"},"reference-count":66,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":29,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Graduate Research Fund of Guizhou Province","award":["2024YJSKYJJ123"],"award-info":[{"award-number":["2024YJSKYJJ123"]}]},{"name":"Graduate Research Fund of Guizhou Province","award":["2024YJSKYJJ122"],"award-info":[{"award-number":["2024YJSKYJJ122"]}]},{"name":"the Science and Technology Research Project of Chongqing Municipal Education Commission","award":["No.202403510"],"award-info":[{"award-number":["No.202403510"]}]},{"name":"Guizhou Provincial Science and Technology Projects","award":["QKHJC-[2025]youth072"],"award-info":[{"award-number":["QKHJC-[2025]youth072"]}]},{"name":"Guizhou Provincial Science and Technology Projects","award":["QKHJC-[2024]youth102"],"award-info":[{"award-number":["QKHJC-[2024]youth102"]}]},{"name":"Basic Research Project of Guizhou University","award":["GDJC-2023\u201312"],"award-info":[{"award-number":["GDJC-2023\u201312"]}]},{"name":"the postgraduate education and teaching reform project of Guizhou Province","award":["2024YJSJGXM005"],"award-info":[{"award-number":["2024YJSJGXM005"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Vet Res"],"DOI":"10.1186\/s13567-026-01730-0","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:34:17Z","timestamp":1777379657000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrative comparative genomics and transcriptomics reveal key roles of SAG17 and SAG23 in early-stage virulence divergence of Eimeria tenella"],"prefix":"10.1186","volume":"57","author":[{"given":"Ye","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongmei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunping","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjie","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liting","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"1730_CR1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.787653","volume":"8","author":"C Mesa-Pineda","year":"2021","unstructured":"Mesa-Pineda C, Navarro-Ru\u00edz JL, L\u00f3pez-Osorio S, Chaparro-Guti\u00e9rrez JJ, G\u00f3mez-Osorio LM (2021) Chicken coccidiosis: from the parasite lifecycle to control of the disease. Front Vet Sci 8:787653","journal-title":"Front Vet Sci"},{"key":"1730_CR2","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1186\/s13567-020-00837-2","volume":"51","author":"DP Blake","year":"2020","unstructured":"Blake DP, Knox J, Dehaeck B, Huntington B, Rathinam T, Ravipati V, Ayoade S, Gilbert W, Adebambo AO, Jatau ID, Raman M, Parker D, Rushton J, Tomley FM\u00a0(2020) Re-calculating the cost of coccidiosis in chickens. Vet Res 51:115","journal-title":"Vet Res"},{"key":"1730_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.molbiopara.2012.08.007","volume":"186","author":"B Cowper","year":"2012","unstructured":"Cowper B, Matthews S, Tomley F (2012) The molecular basis for the distinct host and tissue tropisms of coccidian parasites. Mol Biochem Parasitol 186:1\u201310","journal-title":"Mol Biochem Parasitol"},{"key":"1730_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2025.110473","volume":"337","author":"W Xiang","year":"2025","unstructured":"Xiang W, Chen Y, He Y, Yu Y, Li P, Wang B, Cheng Z\u00a0(2025) The GLI3 may be involved in the damage process of Eimeria tenella to chicken cecal tissue. Vet Parasitol 337:110473","journal-title":"Vet Parasitol"},{"key":"1730_CR5","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1080\/03079457.2022.2133681","volume":"52","author":"B Wang","year":"2023","unstructured":"Wang B, Du P, Huang S, He D, Chen J, Wen X, Yang J, Xian S, Cheng Z\u00a0(2023) Comparison of the caecal microbial community structure and physiological indicators of healthy and infection Eimeria tenella chickens during peak of oocyst shedding. Avian Pathol 52:51\u201361","journal-title":"Avian Pathol"},{"key":"1730_CR6","first-page":"657","volume":"9","author":"L Xu","year":"2022","unstructured":"Xu L, Xiang Q, Li M, Sun X, Lu M, Yan R, Song X, Li X (2022) Pathogenic effects of single or mixed infections of Eimeria mitis, Eimeria necatrix, and Eimeria tenella in chickens. Vet Sci 9:657","journal-title":"Vet Sci"},{"key":"1730_CR7","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-020-00873-y","volume":"52","author":"W Chen","year":"2021","unstructured":"Chen W, Ma C, Li G, Jia Z, Yang X, Pan X, Ma D\u00a0(2021) Specific EtMIC3-binding peptides inhibit Eimeria tenella sporozoites entry into host cells. Vet Res 52:24","journal-title":"Vet Res"},{"key":"1730_CR8","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1111\/j.1365-3024.1988.tb00251.x","volume":"10","author":"V McDonald","year":"1988","unstructured":"McDonald V, Wisher MH, Rose ME, Jeffers TK (1988) Eimeria tenella: immunological diversity between asexual generations. Parasite Immunol 10:649\u2013660","journal-title":"Parasite Immunol"},{"key":"1730_CR9","doi-asserted-by":"publisher","DOI":"10.3390\/life13061295","volume":"13","author":"JD Britez","year":"2023","unstructured":"Britez JD, Rodriguez AE, Di Ciaccio L, Marug\u00e1n-Hernandez V, Tomazic ML (2023) What do we know about surface proteins of chicken parasites Eimeria? Life Basel 13:1295","journal-title":"Life Basel"},{"key":"1730_CR10","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-01904-w","volume":"4","author":"NZ Ramly","year":"2021","unstructured":"Ramly NZ, Dix SR, Ruzheinikov SN, Sedelnikova SE, Baker PJ, Chow YP, Wan KL, Nathan S,\u00a0Rice DW (2021) The structure of a major surface antigen SAG19 from Eimeria tenella unifies the Eimeria SAG family. Commun Biol 4:376","journal-title":"Commun Biol"},{"key":"1730_CR11","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2020068","volume":"27","author":"Q Wang","year":"2020","unstructured":"Wang Q, Zhao Q, Zhu S, Huang B, Yu S, Liang S, Wang H, Zhao H, Han H, Dong H\u00a0(2020) Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1. Parasite 27:70","journal-title":"Parasite"},{"key":"1730_CR12","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00228-21","volume":"9","author":"N Zhao","year":"2021","unstructured":"Zhao N, Ming S, Sun L, Wang B, Li H, Zhang X, Zhao X\u00a0(2021) Identification and characterization of Eimeria tenella microneme protein (EtMIC8). Microbiol Spectr 9:e0022821","journal-title":"Microbiol Spectr"},{"key":"1730_CR13","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1111\/cmi.12181","volume":"16","author":"E Knuepfer","year":"2014","unstructured":"Knuepfer E, Suleyman O, Dluzewski AR, Straschil U, O'Keeffe AH, Ogun SA, Green JL, Grainger M, Tewari R, Holder AA\u00a0(2014) RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation. Cell Microbiol 16:657\u2013672","journal-title":"Cell Microbiol"},{"key":"1730_CR14","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1007\/s00436-009-1437-6","volume":"105","author":"D Jahn","year":"2009","unstructured":"Jahn D, Matros A, Bakulina AY, Tiedemann J, Schubert U, Giersberg M, Haehnel S, Zoufal K, Mock HP, Kipriyanov SM.\u00a0(2009) Model structure of the immunodominant surface antigen of Eimeria tenella identified as a target for sporozoite-neutralizing monoclonal antibody. Parasitol Res 105:655\u2013668","journal-title":"Parasitol Res"},{"key":"1730_CR15","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/S0166-6851(01)00297-3","volume":"116","author":"A Jacquet","year":"2001","unstructured":"Jacquet A, Coulon L, De N\u00e8ve J, Daminet V, Haumont M, Garcia L, Bollen A, Jurado M, Biemans R\u00a0(2001) The surface antigen SAG3 mediates the attachment of Toxoplasma gondii to cell-surface proteoglycans. Mol Biochem Parasitol 116:35\u201344","journal-title":"Mol Biochem Parasitol"},{"key":"1730_CR16","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37431-x","volume":"14","author":"L Koreny","year":"2023","unstructured":"Koreny L, Mercado-Saavedra BN, Klinger CM, Barylyuk K, Butterworth S, Hirst J, Rivera-Cuevas Y, Zaccai NR, Holzer VJC, Klingl A, Dacks JB, Carruthers VB, Robinson MS, Gras S, Waller RF (2023) Stable endocytic structures navigate the complex pellicle of apicomplexan parasites. Nat Commun 14:2167","journal-title":"Nat Commun"},{"key":"1730_CR17","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1016\/j.ceca.2014.12.010","volume":"57","author":"S Lourido","year":"2015","unstructured":"Lourido S, Moreno SN (2015) The calcium signaling toolkit of the Apicomplexan parasites Toxoplasma gondii and Plasmodium spp. Cell Calcium 57:186\u2013193","journal-title":"Cell Calcium"},{"key":"1730_CR18","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1053701","volume":"9","author":"X Wu","year":"2022","unstructured":"Wu XJ, Gao J, Mu BJ, Yu LM, Wang ZR, Zheng WB, Gao WW, Zhu XQ, Liu Q\u00a0(2022) Transcriptomic analysis of LMH cells in response to the overexpression of a protein of Eimeria tenella encoded by the locus ETH_00028350. Front Vet Sci 9:1053701","journal-title":"Front Vet Sci"},{"key":"1730_CR19","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1128\/CMR.15.3.414-429.2002","volume":"15","author":"J Caama\u00f1o","year":"2002","unstructured":"Caama\u00f1o J, Hunter CA (2002) NF-kappaB family of transcription factors: central regulators of innate and adaptive immune functions. Clin Microbiol Rev 15:414\u2013429","journal-title":"Clin Microbiol Rev"},{"key":"1730_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025233","volume":"6","author":"YP Chow","year":"2011","unstructured":"Chow YP, Wan KL, Blake DP, Tomley F, Nathan S (2011) Immunogenic Eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages. PLoS One 6:e25233","journal-title":"PLoS ONE"},{"key":"1730_CR21","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1186\/s13071-018-2906-5","volume":"11","author":"T Liu","year":"2018","unstructured":"Liu T, Huang J, Li Y, Ehsan M, Wang S, Zhou Z, Song X, Yan R, Xu L, Li X\u00a0(2018) Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene. Parasit Vectors 11:325","journal-title":"Parasit Vectors"},{"key":"1730_CR22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.131807","volume":"269","author":"H Yu","year":"2024","unstructured":"Yu H, Tang J, Dong L, Tang M, Arif A, Zhang T, Zhang G, Xie K, Zhao Z, Dai G\u00a0(2024) Transcriptome analysis reveals that gga-miR-2954 inhibits the inflammatory response against Eimeria tenella infection. Int J Biol Macromol 269:131807","journal-title":"Int J Biol Macromol"},{"key":"1730_CR23","doi-asserted-by":"publisher","DOI":"10.3390\/ani14233524","volume":"14","author":"S Fang","year":"2024","unstructured":"Fang S, Meng L, Shi Y, Hao C, Gu X, Du F, Cui P, Tang X\u00a0(2024) Comparative analysis of cross-protective immunity among three geographically distinct isolates of Eimeria kongi. Animals (Basel) 14:3524","journal-title":"Animals (Basel)"},{"key":"1730_CR24","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.vetpar.2005.11.024","volume":"136","author":"A Haug","year":"2006","unstructured":"Haug A, Williams RB, Larsen S (2006) Counting coccidial oocysts in chicken faeces: a comparative study of a standard McMaster technique and a new rapid method. Vet Parasitol 136:233\u2013242","journal-title":"Vet Parasitol"},{"key":"1730_CR25","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2025.106343","volume":"311","author":"J Yang","year":"2025","unstructured":"Yang J, Wang X, Chen Y, He Y, Li P, Wen X, Wang B\u00a0(2025) ChangQing compound relieves Eimeria tenella infection symptoms by modulating intestinal probiotic and pathogenic bacteria balance. Vet J 311:106343","journal-title":"Vet J"},{"key":"1730_CR26","unstructured":"Eimeria tenella Houghton strain genome assembly ETH001 (RefSeq GCF_000499545.2). https:\/\/www.ncbi.nlm.nih.gov\/datasets\/genome\/GCF_000499545.2\/. Accessed 6 Nov 2013."},{"key":"1730_CR27","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.1923","volume":"9","author":"B Langmead","year":"2012","unstructured":"Langmead B, Salzberg SL (2012) Fast gapped-read alignment with Bowtie 2. Nat Methods 9:357\u2013359","journal-title":"Nat Methods"},{"key":"1730_CR28","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008","volume":"10","author":"P Danecek","year":"2021","unstructured":"Danecek P, Bonfield JK, Liddle J, Marshall J, Ohan V, Pollard MO, Whitwham A, Keane T, McCarthy SA, Davies RM, Li H\u00a0(2021) Twelve years of SAMtools and BCFtools. Gigascience 10:giab008","journal-title":"Gigascience"},{"key":"1730_CR29","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1101\/gr.129684.111","volume":"22","author":"DC Koboldt","year":"2012","unstructured":"Koboldt DC, Zhang Q, Larson DE, Shen D, McLellan MD, Lin L, Miller CA, Mardis ER, Ding L, Wilson RK\u00a0(2012) VarScan 2: somatic mutation and copy number alteration discovery in cancer by exome sequencing. Genome Res 22:568\u2013576","journal-title":"Genome Res"},{"key":"1730_CR30","doi-asserted-by":"publisher","first-page":"80","DOI":"10.4161\/fly.19695","volume":"6","author":"P Cingolani","year":"2012","unstructured":"Cingolani P, Platts A, Wang L, Coon M, Nguyen T, Wang L, Land SJ, Lu X, Ruden DM (2012) A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly (Austin) 6:80\u201392","journal-title":"Fly (Austin)"},{"key":"1730_CR31","doi-asserted-by":"publisher","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","volume":"30","author":"AM Bolger","year":"2014","unstructured":"Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114\u20132120","journal-title":"Bioinformatics"},{"key":"1730_CR32","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/nmeth.3317","volume":"12","author":"D Kim","year":"2015","unstructured":"Kim D, Langmead B, Salzberg SL (2015) HISAT: a fast spliced aligner with low memory requirements. Nat Methods 12:357\u2013360","journal-title":"Nat Methods"},{"key":"1730_CR33","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/nmeth.1226","volume":"5","author":"A Mortazavi","year":"2008","unstructured":"Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods 5:621\u2013628","journal-title":"Nat Methods"},{"key":"1730_CR34","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","volume":"31","author":"S Anders","year":"2015","unstructured":"Anders S, Pyl PT, Huber W (2015) HTSeq\u2013a Python framework to work with high-throughput sequencing data. Bioinformatics 31:166\u2013169","journal-title":"Bioinformatics"},{"key":"1730_CR35","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","volume":"26","author":"MD Robinson","year":"2010","unstructured":"Robinson MD, McCarthy DJ, Smyth GK (2010) edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26:139\u2013140","journal-title":"Bioinformatics"},{"key":"1730_CR36","unstructured":"SignalP 5.0 Server. https:\/\/services.healthtech.dtu.dk\/services\/SignalP-5.0\/."},{"key":"1730_CR37","unstructured":"TMHMM 2.0 Server. https:\/\/services.healthtech.dtu.dk\/service.php?TMHMM-2.0."},{"key":"1730_CR38","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25:402\u2013408","journal-title":"Methods"},{"key":"1730_CR39","unstructured":"Prokaryotic Protein Expression Service. https:\/\/www.zoonbio.com\/protein-expression-service-index-bacteria-example.html."},{"key":"1730_CR40","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1038\/227680a0","volume":"227","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680\u2013685","journal-title":"Nature"},{"key":"1730_CR41","doi-asserted-by":"publisher","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","volume":"76","author":"H Towbin","year":"1979","unstructured":"Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76:4350\u20134354","journal-title":"Proc Natl Acad Sci U S A"},{"key":"1730_CR42","doi-asserted-by":"publisher","first-page":"4384","DOI":"10.3382\/ps\/pez218","volume":"98","author":"L Zhang","year":"2019","unstructured":"Zhang L, Zheng MX, Xi R, Xu ZY, Zhang XS, Zheng LL, Bai R, Mi CL, Hao FF, Feng YP\u00a0(2019) Comparison of the host cells apoptosis induced by precocious strains and virulent strains of Eimeria tenella. Poult Sci 98:4384\u20134390","journal-title":"Poult Sci"},{"key":"1730_CR43","doi-asserted-by":"publisher","first-page":"2549","DOI":"10.1007\/s00436-020-06738-9","volume":"119","author":"X Zhang","year":"2020","unstructured":"Zhang X, Li S, Zheng M, Zhang L, Bai R, Li R, Hao S, Bai B, Kang H\u00a0(2020) Effects of the PI3K\/Akt signaling pathway on the apoptosis of early host cells infected with Eimeria tenella. Parasitol Res 119:2549\u20132561","journal-title":"Parasitol Res"},{"key":"1730_CR44","doi-asserted-by":"publisher","first-page":"2970","DOI":"10.3382\/ps\/pev293","volume":"94","author":"Y Zhang","year":"2015","unstructured":"Zhang Y, Zheng MX, Xu ZY, Xu HC, Cui XZ, Yang SS, Zhao WL, Li S, Lv QH, Bai R\u00a0(2015) Relationship between Eimeria tenella development and host cell apoptosis in chickens. Poult Sci 94:2970\u20132979","journal-title":"Poult Sci"},{"key":"1730_CR45","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1007\/s11250-011-0024-x","volume":"44","author":"MM Awais","year":"2012","unstructured":"Awais MM, Akhtar M, Iqbal Z, Muhammad F, Anwar MI (2012) Seasonal prevalence of coccidiosis in industrial broiler chickens in Faisalabad, Punjab, Pakistan. Trop Anim Health Prod 44:323\u2013328","journal-title":"Trop Anim Health Prod"},{"key":"1730_CR46","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.rvsc.2010.10.023","volume":"92","author":"SS Abu-Akkada","year":"2012","unstructured":"Abu-Akkada SS, Awad AM (2012) Isolation, propagation, identification and comparative pathogenicity of five Egyptian field strains of Eimeria tenella from broiler chickens in five different provinces in Egypt. Res Vet Sci 92:92\u201395","journal-title":"Res Vet Sci"},{"key":"1730_CR47","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02732","volume":"10","author":"WH Kim","year":"2019","unstructured":"Kim WH, Chaudhari AA, Lillehoj HS (2019) Involvement of T cell immunity in avian coccidiosis. Front Immunol 10:2732","journal-title":"Front Immunol"},{"key":"1730_CR48","doi-asserted-by":"publisher","first-page":"2785","DOI":"10.1021\/acs.jproteome.2c00726","volume":"22","author":"Z Gong","year":"2023","unstructured":"Gong Z, Qu Z, Yu Z, Li J, Liu B, Ma X, Cai J\u00a0(2023) Label-free quantitative detection and comparative analysis of lysine acetylation during the different life stages of Eimeria tenella. J Proteome Res 22:2785\u20132802","journal-title":"J Proteome Res"},{"key":"1730_CR49","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012354","volume":"5","author":"MS Behnke","year":"2010","unstructured":"Behnke MS, Wootton JC, Lehmann MM, Radke JB, Lucas O, Nawas J, Sibley LD, White MW\u00a0(2010) Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii. PLoS One 5:e12354","journal-title":"PLoS ONE"},{"key":"1730_CR50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0000005","volume":"1","author":"Z Bozdech","year":"2003","unstructured":"Bozdech Z, Llin\u00e1s M, Pulliam BL, Wong ED, Zhu J, DeRisi JL (2003) The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum. PLoS Biol 1:E5","journal-title":"PLoS Biol"},{"key":"1730_CR51","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1017\/S0031182003004736","volume":"128","author":"SA Robinson","year":"2004","unstructured":"Robinson SA, Smith JE, Millner PA (2004) Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding. Parasitology 128:385\u2013394","journal-title":"Parasitology"},{"key":"1730_CR52","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.molbiopara.2004.01.013","volume":"135","author":"E Tabar\u00e9s","year":"2004","unstructured":"Tabar\u00e9s E, Ferguson D, Clark J, Soon PE, Wan KL, Tomley F (2004) Eimeria tenella sporozoites and merozoites differentially express glycosylphosphatidylinositol-anchored variant surface proteins. Mol Biochem Parasitol 135:123\u2013132","journal-title":"Mol Biochem Parasitol"},{"key":"1730_CR53","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1111\/j.1462-5822.2011.01597.x","volume":"13","author":"S Besteiro","year":"2011","unstructured":"Besteiro S, Dubremetz JF, Lebrun M (2011) The moving junction of apicomplexan parasites: a key structure for invasion. Cell Microbiol 13:797\u2013805","journal-title":"Cell Microbiol"},{"key":"1730_CR54","doi-asserted-by":"publisher","first-page":"4566","DOI":"10.1002\/pmic.200900305","volume":"9","author":"K Lal","year":"2009","unstructured":"Lal K, Bromley E, Oakes R, Prieto JH, Sanderson SJ, Kurian D, Hunt L, Yates JR 3rd, Wastling JM, Sinden RE, Tomley FM\u00a0(2009) Proteomic comparison of four Eimeria tenella life-cycle stages: unsporulated oocyst, sporulated oocyst, sporozoite and second-generation merozoite. Proteomics 9:4566\u20134576","journal-title":"Proteomics"},{"key":"1730_CR55","doi-asserted-by":"publisher","first-page":"1467","DOI":"10.1242\/jcs.111.11.1467","volume":"111","author":"K Lingelbach","year":"1998","unstructured":"Lingelbach K, Joiner KA (1998) The parasitophorous vacuole membrane surrounding Plasmodium and Toxoplasma: an unusual compartment in infected cells. J Cell Sci 111:1467\u20131475","journal-title":"J Cell Sci"},{"key":"1730_CR56","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1186\/s12866-022-02512-2","volume":"22","author":"MS Barbosa","year":"2022","unstructured":"Barbosa MS, Marques LM, Timenetsky J, Rosengarten R, Spergser J, Chopra-Dewasthaly R (2022) Host cell interactions of novel antigenic membrane proteins of Mycoplasma agalactiae. BMC Microbiol 22:93","journal-title":"BMC Microbiol"},{"key":"1730_CR57","doi-asserted-by":"publisher","first-page":"556","DOI":"10.3382\/ps\/pey404","volume":"98","author":"WY Li","year":"2019","unstructured":"Li W, Liu D, Tang S, Li D, Han R, Tian Y, Li H, Li G, Li W, Liu X, Kang X, Li Z\u00a0(2019) A multiallelic InDel in the promoter region of the cyclin-dependent kinase inhibitor 3 gene is significantly associated with body weight and carcass traits in chickens. Poult Sci 98:556\u2013565","journal-title":"Poult Sci"},{"key":"1730_CR58","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2667","volume":"10","author":"R Rajkumar","year":"2013","unstructured":"Rajkumar R, Tyagi S, van Oudenaarden A (2013) Binding-site affinities tune promoter output. Nat Methods 10:933","journal-title":"Nat Methods"},{"key":"1730_CR59","first-page":"234","volume":"103","author":"L Zhang","year":"2023","unstructured":"Zhang L, Song Z, Li P, Song X, Tang X, Hu D\u00a0(2023) Molecular characterization and immune protective efficacy of three Eimeria tenella antigens. Poult Sci 103:103234","journal-title":"Poult Sci"},{"key":"1730_CR60","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1038\/s41576-020-0269-1","volume":"21","author":"D Ebert","year":"2020","unstructured":"Ebert D, Fields PD (2020) Host-parasite co-evolution and its genomic signature. Nat Rev Genet 21:754\u2013768","journal-title":"Nat Rev Genet"},{"issue":"2","key":"1730_CR61","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1093\/evolut\/qpaf238","volume":"80","author":"DD Patterson","year":"2025","unstructured":"Patterson DD, Childs LM, Stopard IJ, Chitnis N, Serrato-Arroyo S, Greischar M (2025) Immunity can impose a reproduction-survival tradeoff on human malaria parasites. Evolution 80:396\u2013412","journal-title":"Evolution"},{"key":"1730_CR62","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.meegid.2016.02.021","volume":"40","author":"M Matsubayashi","year":"2016","unstructured":"Matsubayashi M, Kawahara F, Hatta T, Yamagishi J, Miyoshi T, Anisuzzaman, Sasai K, Isobe T, Kita K, Tsuji N\u00a0(2016) Transcriptional profiles of virulent and precocious strains of Eimeria tenella at sporozoite stage; novel biological insight into attenuated asexual development. Infect Genet Evol 40:54-62","journal-title":"Infect Genet Evol"},{"key":"1730_CR63","unstructured":"PRJNA1163523 (Eimeria tenella Beijing strain, Gallus gallus). https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1163523\/. Accessed 21 Sep 2024."},{"key":"1730_CR64","unstructured":"PRJNA1327252 (Eimeria tenella Guizhou strain, Gallus gallus). https:\/\/www.ncbi.nlm.nih.gov\/bioproject\/PRJNA1327252\/. Accessed 11 Sep 2025."},{"key":"1730_CR65","unstructured":"SRR35396008 (Eimeria tenella Beijing strain, Gallus gallus). https:\/\/www.ncbi.nlm.nih.gov\/sra\/SRR35396008\/. Accessed 16 Feb 2026."},{"key":"1730_CR66","unstructured":"SRR35396007 (Eimeria tenella Guizhou strain, Gallus gallus). https:\/\/www.ncbi.nlm.nih.gov\/sra\/SRR35396007\/. Accessed 16 Feb 2026."}],"container-title":["Veterinary Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13567-026-01730-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-026-01730-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13567-026-01730-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:02:24Z","timestamp":1779883344000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s13567-026-01730-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":66,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["1730"],"URL":"https:\/\/doi.org\/10.1186\/s13567-026-01730-0","relation":{},"ISSN":["1297-9716"],"issn-type":[{"value":"1297-9716","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]},"assertion":[{"value":"27 October 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 April 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All animal experiments were approved by the Animal Ethics Committee of Guizhou University and conducted in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals of Guizhou Province. In vivo infection experiments and transcriptome analyses were carried out in compliance with the ethical guidelines approved under protocol no. EAE-GZU-2024-T069, EAE-GZU-2024-E058.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"86"}}