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Genetic selection for\n                    <jats:italic>PRNP<\/jats:italic>\n                    alleles associated with resistance to classical scrapie, particularly K\n                    <jats:sub>222<\/jats:sub>\n                    and S\n                    <jats:sub>127<\/jats:sub>\n                    , has been proposed as a control strategy, but their effect on caprine BSE is unclear. Nine Alpine goats carrying different\n                    <jats:italic>PRNP<\/jats:italic>\n                    genotypes at codons\n                    <jats:sub>222<\/jats:sub>\n                    (Q\/Q, Q\/K, K\/K) and\n                    <jats:sub>127<\/jats:sub>\n                    (G\/G, G\/S) were intracerebrally inoculated with a second-passage caprine BSE isolate and monitored until terminal disease or censoring. Brain and selected peripheral tissues were examined for PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    by immunohistochemistry, western blotting, and conformation-dependent immunoassay, and lesion severity and PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    distribution were compared among genotypes. All goats lacking the S\n                    <jats:sub>127<\/jats:sub>\n                    allele developed clinical disease with widespread PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    accumulation in the central nervous system. Compared with Q\/Q\n                    <jats:sub>222<\/jats:sub>\n                    goats, Q\/K\n                    <jats:sub>222<\/jats:sub>\n                    and K\/K\n                    <jats:sub>222<\/jats:sub>\n                    goats showed longer incubation periods and lower overall PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    and vacuolation scores, although disease still occurred in both genotypes after intracerebral challenge. Two G\/S\n                    <jats:sub>127<\/jats:sub>\n                    goats, one Q\/Q\n                    <jats:sub>222<\/jats:sub>\n                    and one Q\/K\n                    <jats:sub>222<\/jats:sub>\n                    , remained clinically healthy and biopsy-negative at\u2009&gt;\u20091400\u00a0days post-inoculation, but were right-censored because they were alive at last follow-up and had not undergone post\u00a0mortem examination. PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    was also variably detected in lymphoreticular tissues and in the enteric and peripheral nervous systems, irrespective of genotype. Under these stringent challenge conditions, K\n                    <jats:sub>222<\/jats:sub>\n                    delays disease onset but does not confer complete resistance. The prolonged survival of S\n                    <jats:sub>127<\/jats:sub>\n                    carriers remains a preliminary, censored observation requiring post mortem confirmation.\n                  <\/jats:p>","DOI":"10.1186\/s13567-026-01760-8","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:24:06Z","timestamp":1777566246000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Incomplete protection of the K222 polymorphism of the prion protein gene after intracerebral exposure to caprine BSE in goats: preliminary follow-up in S127 carriers"],"prefix":"10.1186","volume":"57","author":[{"given":"Diego","family":"Sola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9-Luis","family":"Pitarch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9n","family":"Mar\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen-Caroline","family":"Raksa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Otero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Barillet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederic","family":"Bouvier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Bolea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Bossers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Andreoletti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Langeveld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan-Jos\u00e9","family":"Badiola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Ac\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"1760_CR1","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1126\/science.6801762","volume":"216","author":"SB Prusiner","year":"1982","unstructured":"Prusiner SB (1982) Novel proteinaceous infectious particles cause scrapie. 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