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When provided with corn syrup and vitamin B<jats:sub>12<\/jats:sub> (0.03\u00a0mol B<jats:sub>12<\/jats:sub> per mol CF), DHM-1 grew and biodegraded up to 2,000\u00a0mg\/L of CF in 180\u00a0days, with only minor transient accumulation of dichloromethane and chloromethane. CT (15\u00a0mg\/L) and CFC-11 (25\u00a0mg\/L) were also biodegraded without significant accumulation of halomethane daughter products. The rate of CF biodegradation followed a Michaelis-Menten-like pattern with respect to the B<jats:sub>12<\/jats:sub> concentration; one-half the maximum rate (66\u00a0mg CF\/L\/d) occurred at 0.005\u00a0mol B<jats:sub>12<\/jats:sub> per mol CF. DHM-1 was able to biodegrade 500\u00a0mg\/L of CF at an inoculum level as low as 10<jats:sup>\u22128<\/jats:sup>\u00a0mg protein\/L. The highest rate of CF biodegradation occurred at pH\u00a07.7; activity decreased substantially below pH\u00a06.0. DHM-1 biodegraded mixtures of CT, CFC-11, and CF, although CFC-11 inhibited CF biodegradation. Evidence for compete defluorination of CFC-11 was obtained based on a fluoride mass balance. Overall, the results suggest that DHM-1 may be effective for bioaugmentation in source zones contaminated with thousands of milligrams per liter of CF and tens of milligrams per liter of CT and CFC-11.<\/jats:p>","DOI":"10.1186\/s13568-014-0048-5","type":"journal-article","created":{"date-parts":[[2014,6,13]],"date-time":"2014-06-13T10:34:39Z","timestamp":1402655679000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biodegradation of high concentrations of halomethanes by a fermentative enrichment culture"],"prefix":"10.1186","volume":"4","author":[{"given":"Huifeng","family":"Shan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya","family":"Jacob","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L","family":"Freedman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,6,14]]},"reference":[{"key":"48_CR1","unstructured":"ATSDR (2013) The ATSDR 2011 Substance Priority List. 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