{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:53:41Z","timestamp":1762102421014,"version":"3.37.3"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T00:00:00Z","timestamp":1567036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T00:00:00Z","timestamp":1567036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/26058-0","2017\/50332-0"],"award-info":[{"award-number":["2015\/26058-0","2017\/50332-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["315236\/2018-3"],"award-info":[{"award-number":["315236\/2018-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["AMB Expr"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s13568-019-0856-8","type":"journal-article","created":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T11:03:21Z","timestamp":1567076601000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis"],"prefix":"10.1186","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2617-7739","authenticated-orcid":false,"given":"Felipe D.","family":"Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6937-1120","authenticated-orcid":false,"given":"Jo\u00e3o E.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3926-5981","authenticated-orcid":false,"given":"Renan P.","family":"Freire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7467-3457","authenticated-orcid":false,"given":"Miriam F.","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7982-1789","authenticated-orcid":false,"given":"Carlos R.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5258-1527","authenticated-orcid":false,"given":"Paolo","family":"Bartolini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,8,29]]},"reference":[{"issue":"8","key":"856_CR1","doi-asserted-by":"publisher","first-page":"2212","DOI":"10.1007\/s12010-012-9745-1","volume":"167","author":"FS Arthuso","year":"2012","unstructured":"Arthuso FS, Bartolini P, Soares CRJ (2012) Laboratory production of human prolactin from CHO cells adapted to serum-free suspension culture. Appl Biochem Biotech 167(8):2212\u20132224","journal-title":"Appl Biochem Biotech"},{"issue":"1","key":"856_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1677\/JOE-10-0069","volume":"206","author":"S Bernichtein","year":"2010","unstructured":"Bernichtein S, Touraine P, Goffin V (2010) New concepts in prolactin biology. J Endocrinol 206(1):1\u201311. https:\/\/doi.org\/10.1677\/JOE-10-0069","journal-title":"J Endocrinol"},{"key":"856_CR003","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s12896-015-0205-1","volume":"15","author":"E Bohm","year":"2015","unstructured":"Bohm E, Seyfried BK, Dockal M, Graninger M, Hasslacher M, Neurath M, Konetschny C, Matthiessen P, Mitterer A, Scheiflinger F (2015) Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells. BMC Biotechnol 15:87. https:\/\/doi.org\/10.1186\/s12896-015-0205-1","journal-title":"BMC Biotechnol"},{"issue":"3","key":"856_CR3","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1210\/edrv.19.3.0334","volume":"19","author":"C Bole-Feysot","year":"1998","unstructured":"Bole-Feysot C, Goffin V, Edery M, Binart N, Kelly PA (1998) Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev 19(3):225\u2013268","journal-title":"Endocr Rev"},{"issue":"1","key":"856_CR01","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1186\/s12896-018-0485-3","volume":"18","author":"J Bouvette","year":"2018","unstructured":"Bouvette J, Korkut DN, Fouillen A, Amellah S, Nanci A, Durocher Y, Omichinski JG, Legault P (2018) High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension. BMC Biotechnol 18(1):76. https:\/\/doi.org\/10.1186\/s12896-018-0485-3","journal-title":"BMC Biotechnol"},{"key":"856_CR4","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.jbiotec.2014.11.034","volume":"202","author":"MV Capone","year":"2015","unstructured":"Capone MV, Suzuki MF, Oliveira JE, Damiani R, Soares CR, Bartolini P (2015) N-glycoprofiling analysis in a simple glycoprotein model: a comparison between recombinant and pituitary glycosylated human prolactin. J Biotechnol 202:78\u201387. https:\/\/doi.org\/10.1016\/j.jbiotec.2014.11.034","journal-title":"J Biotechnol"},{"issue":"3","key":"856_CR02","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.jbiotec.2012.06.038","volume":"161","author":"A Croset","year":"2012","unstructured":"Croset A, Delafosse L, Gaudry JP, Arod C, Glez L, Losberger C, Begue D, Krstanovic A, Robert F, Vilbois F, Chevalet L, Antonsson B (2012) Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells. J Biotechnol 161(3):336\u2013348. https:\/\/doi.org\/10.1016\/j.jbiotec.2012.06.038","journal-title":"J Biotechnol"},{"issue":"5","key":"856_CR03","doi-asserted-by":"publisher","first-page":"1889","DOI":"10.1007\/s00253-016-7973-y","volume":"101","author":"K Ding","year":"2017","unstructured":"Ding K, Han L, Zong H, Chen J, Zhang B, Zhu J (2017) Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein. Appl Microbiol Biotechnol 101(5):1889\u20131898. https:\/\/doi.org\/10.1007\/s00253-016-7973-y","journal-title":"Appl Microbiol Biotechnol"},{"issue":"6","key":"856_CR04","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1016\/j.copbio.2009.10.008","volume":"20","author":"Y Durocher","year":"2009","unstructured":"Durocher Y, Butler M (2009) Expression systems for therapeutic glycoprotein production. Curr Opin Biotechnol 20(6):700\u2013707. https:\/\/doi.org\/10.1016\/j.copbio.2009.10.008","journal-title":"Curr Opin Biotechnol"},{"key":"856_CR5","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1111\/j.1365-2826.2010.02011.x","volume":"22","author":"I Fernandez","year":"2010","unstructured":"Fernandez I, Touraine P, Goffin V (2010) Prolactin and human tumourigenesis. J Neuroendocrinol 22:771\u2013777","journal-title":"J Neuroendocrinol"},{"issue":"4","key":"856_CR6","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1159\/000337783","volume":"77","author":"HL Fideleff","year":"2012","unstructured":"Fideleff HL, Sequera AM, Ruibal GF, Boquete HR, Suarez MG, Colombani M, Azaretzky M, Brunetto O, Scaglia HE (2012) Cosecretion of glycosylated prolactin and non-glycosylated prolactin from childhood to the end of puberty. Horm Res Paediatr 77(4):229\u2013234","journal-title":"Horm Res Paediatr"},{"issue":"6","key":"856_CR05","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/j.nbt.2014.11.005","volume":"32","author":"L Fliedl","year":"2015","unstructured":"Fliedl L, Grillari J, Grillari-Voglauer R (2015) Human cell lines for the production of recombinant proteins: on the horizon. New Biotechnol 32(6):673\u2013679. https:\/\/doi.org\/10.1016\/j.nbt.2014.11.005","journal-title":"New Biotechnol"},{"issue":"4","key":"856_CR7","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1152\/physrev.2000.80.4.1523","volume":"80","author":"ME Freeman","year":"2000","unstructured":"Freeman ME, Kanyicska B, Lerant A, Nagy G (2000) Prolactin: structure, function, and regulation of secretion. Physiol Rev 80(4):1523\u20131631","journal-title":"Physiol Rev"},{"issue":"3","key":"856_CR8","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1002\/ajpa.22058","volume":"148","author":"LT Gettler","year":"2012","unstructured":"Gettler LT, McDade TW, Feranil AB, Kuzawa CW (2012) Prolactin, fatherhood, and reproductive behavior in human males. Am J Phys Anthropol 148(3):362\u2013370. https:\/\/doi.org\/10.1002\/ajpa.22058","journal-title":"Am J Phys Anthropol"},{"issue":"3","key":"856_CR9","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1210\/jc.2005-1831","volume":"91","author":"A Glezer","year":"2006","unstructured":"Glezer A, Soares CR, Vieira JG, Giannella-Neto D, Ribela MT, Goffin V, Bronstein MD (2006) Human macroprolactin displays low biological activity via its homologous receptor in a new sensitive bioassay. J Clin Endocrinol Metab 91(3):1048\u20131055.","journal-title":"J Clin Endocrinol Metab"},{"key":"856_CR10","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1146\/annurev.physiol.64.081501.131049","volume":"64","author":"V Goffin","year":"2002","unstructured":"Goffin V, Binart N, Touraine P, Kelly PA (2002) Prolactin: the new biology of an old hormone. Annu Rev Physiol 64:47\u201367. https:\/\/doi.org\/10.1146\/annurev.physiol.64.081501.131049","journal-title":"Annu Rev Physiol"},{"key":"856_CR06","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.ejpb.2016.11.012","volume":"112","author":"A Gugliotta","year":"2017","unstructured":"Gugliotta A, Ceaglio N, Raud B, Forno G, Mauro L, Kratje R, Oggero M (2017) Glycosylation and antiproliferative activity of hyperglycosylated IFN-\u03b12 potentiate HEK293 cells as biofactories. Eur J Pharm Biopharm 112:119\u2013131. https:\/\/doi.org\/10.1016\/j.ejpb.2016.11.012","journal-title":"Eur J Pharm Biopharm"},{"issue":"2","key":"856_CR11","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1210\/jcem-71-2-379","volume":"71","author":"LS Haro","year":"1990","unstructured":"Haro LS, Lee DW, Singh RN, Bee G, Markoff E, Lewis UJ (1990) Glycosylated human prolactin: alterations in glycosylation pattern modify affinity for lactogen receptor and values in prolactin radioimmunoassay. J Clin Endocrinol Metab 71(2):379\u2013383. https:\/\/doi.org\/10.1210\/jcem-71-2-379","journal-title":"J Clin Endocrinol Metab"},{"issue":"4","key":"856_CR12","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.jbiotec.2009.12.019","volume":"145","author":"SR Heller","year":"2010","unstructured":"Heller SR, Rodrigues Goulart H, Arthuso FS, Oliveira TL, Bartolini P, Soares CR (2010) Synthesis, purification and characterization of recombinant glycosylated human prolactin (G-hPRL) secreted by cycloheximide-treated CHO cells. J Biotechnol 145(4):334\u2013340. https:\/\/doi.org\/10.1016\/j.jbiotec.2009.12.019","journal-title":"J Biotechnol"},{"issue":"1","key":"856_CR07","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1159\/000485501","volume":"205","author":"J Hu","year":"2018","unstructured":"Hu J, Han J, Li H, Zhang X, Liu LL, Chen F, Zeng B (2018) Human embryonic kidney 293 cells: a vehicle for biopharmaceutical manufacturing, structural biology, and electrophysiology. Cells Tissues Organs 205(1):1\u20138. https:\/\/doi.org\/10.1159\/000485501","journal-title":"Cells Tissues Organs"},{"issue":"6","key":"856_CR08","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1080\/10826068.2018.1479865","volume":"49","author":"M Mortazavi","year":"2019","unstructured":"Mortazavi M, Shokrgozar MA, Sardari S, Azadmanesh K, Mahdian R, Kaghazian H, Hosseini SN, Hedayati MH (2019) Using chemical chaperones to increase recombinant human erythropoietin secretion in CHO cell line. Prep Biochem Biotechnol 49(6):535\u2013544. https:\/\/doi.org\/10.1080\/10826068.2018.1479865","journal-title":"Prep Biochem Biotechnol"},{"issue":"11","key":"856_CR13","doi-asserted-by":"publisher","first-page":"4827","DOI":"10.1210\/endo.136.11.7588213","volume":"136","author":"AE Price","year":"1995","unstructured":"Price AE, Logvinenko KB, Higgins EA, Cole ES, Richards SM (1995) Studies on the microheterogeneity and in vitro activity of glycosylated and nonglycosylated recombinant human prolactin separated using a novel purification process. Endocrinology 136(11):4827\u20134833. https:\/\/doi.org\/10.1210\/endo.136.11.7588213","journal-title":"Endocrinology"},{"key":"856_CR14","volume-title":"Draft report of an international collaborative study of the proposed WHO reference reagents for rDNA-derived prolactin and its glycosylated and non-glycosylated components","author":"B Rafferty","year":"2001","unstructured":"Rafferty B, Rigsby P, Gaines-Das RE (2001) Draft report of an international collaborative study of the proposed WHO reference reagents for rDNA-derived prolactin and its glycosylated and non-glycosylated components. WHO, Genova"},{"key":"856_CR15","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18020131","author":"MT Ribela","year":"2017","unstructured":"Ribela MT, Damiani R, Silva FD, Lima ER, Oliveira JE, Peroni CN, Torjesen PA, Soares CR, Bartolini P (2017) N-Glycoprofiling analysis for carbohydrate composition and site-occupancy determination in a poly-glycosylated protein: human thyrotropin of different origins. Int J Mol Sci. https:\/\/doi.org\/10.3390\/ijms18020131","journal-title":"Int J Mol Sci"},{"issue":"3","key":"856_CR16","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1007\/s00253-017-8684-8","volume":"102","author":"PM Sant\u2019Ana","year":"2018","unstructured":"Sant\u2019Ana PM, Oliveira JE, Lima ER, Soares CRJ, Peroni CN, Bartolini P, Ribela M (2018) Human thyroid-stimulating hormone synthesis in human embryonic kidney cells and related N-glycoprofiling analysis for carbohydrate composition determination. Appl Microbiol Biotech 102(3):1215\u20131228. https:\/\/doi.org\/10.1007\/s00253-017-8684-8","journal-title":"Appl Microbiol Biotech"},{"issue":"1\u20133","key":"856_CR17","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1007\/BF00762394","volume":"15","author":"M Shelikoff","year":"1994","unstructured":"Shelikoff M, Sinskey AJ, Stephanopoulos G (1994) The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin. Cytotechnology 15(1\u20133):195\u2013208","journal-title":"Cytotechnology"},{"issue":"3","key":"856_CR18","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1210\/edrv-16-3-354","volume":"16","author":"YN Sinha","year":"1995","unstructured":"Sinha YN (1995) Structural variants of prolactin: occurrence and physiological significance. Endocr Rev 16(3):354\u2013369","journal-title":"Endocr Rev"},{"issue":"Pt 2","key":"856_CR19","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1042\/BA20000047","volume":"32","author":"CR Soares","year":"2000","unstructured":"Soares CR, Morganti L, Miloux B, Lupker JH, Ferrara P, Bartolini P (2000) High-level synthesis of human prolactin in Chinese-Hamster ovary cells. Biotechnol Appl Biochem 32(Pt 2):127\u2013135","journal-title":"Biotechnol Appl Biochem"},{"issue":"2","key":"856_CR20","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.pep.2006.04.013","volume":"48","author":"CR Soares","year":"2006","unstructured":"Soares CR, Glezer A, Okazaki K, Ueda EK, Heller SR, Walker AM, Goffin V, Bartolini P (2006) Physico-chemical and biological characterizations of two human prolactin analogs exhibiting controversial bioactivity, synthesized in Chinese hamster ovary (CHO) cells. Protein Expr Purif 48(2):182\u2013194.","journal-title":"Protein Expr Purif"},{"issue":"3","key":"856_CR21","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1002\/bab.1008","volume":"59","author":"MF Suzuki","year":"2012","unstructured":"Suzuki MF, Arthuso FS, Oliveira JE, Oliveira NAJ, Goulart HR, Capone MVN, Ribela M, Bartolini P, Soares CRJ (2012) Expression, purification, and characterization of authentic mouse prolactin obtained in Escherichia coli periplasmic space. Biotechnol Appl Bioc 59(3):178\u2013185. https:\/\/doi.org\/10.1002\/bab.1008","journal-title":"Biotechnol Appl Bioc"},{"issue":"1","key":"856_CR09","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.pep.2012.04.023","volume":"84","author":"K Swiech","year":"2012","unstructured":"Swiech K, Picanco-Castro V, Covas DT (2012) Human cells: new platform for recombinant therapeutic protein production. Protein Expr Purif 84(1):147\u2013153. https:\/\/doi.org\/10.1016\/j.pep.2012.04.023","journal-title":"Protein Expr Purif"},{"issue":"18","key":"856_CR22","doi-asserted-by":"publisher","first-page":"6814","DOI":"10.1158\/0008-5472.CAN-04-1870","volume":"64","author":"SS Tworoger","year":"2004","unstructured":"Tworoger SS, Eliassen AH, Rosner B, Sluss P, Hankinson SE (2004) Plasma prolactin concentrations and risk of postmenopausal breast cancer. Cancer Res 64(18):6814\u20136819. https:\/\/doi.org\/10.1158\/0008-5472.CAN-04-1870","journal-title":"Cancer Res"},{"issue":"24","key":"856_CR23","doi-asserted-by":"publisher","first-page":"7561","DOI":"10.1021\/bi034217s","volume":"42","author":"W Wu","year":"2003","unstructured":"Wu W, Coss D, Lorenson MY, Kuo CB, Xu X, Walker AM (2003) Different biological effects of unmodified prolactin and a molecular mimic of phosphorylated prolactin involve different signaling pathways. Biochemistry 42(24):7561\u20137570. https:\/\/doi.org\/10.1021\/bi034217s","journal-title":"Biochemistry"},{"issue":"6","key":"856_CR001","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1016\/j.ymben.2010.08.004","volume":"12","author":"P Zhang","year":"2010","unstructured":"Zhang P, Tan DL, Heng D, Wang T, Mariati, Yang Y, Song Z (2010) A functional analysis of N-glycosylation-related genes on sialylation of recombinant erythropoietin in six commonly used mammalian cell lines. Metab Eng 12(6):526\u2013536. https:\/\/doi.org\/10.1016\/j.ymben.2010.08.004","journal-title":"Metab Eng"},{"issue":"1","key":"856_CR002","doi-asserted-by":"publisher","first-page":"e2724","DOI":"10.1002\/btpr.2724","volume":"35","author":"X Zhong","year":"2019","unstructured":"Zhong X, Ma W, Meade CL, Tam AS, Llewellyn E, Cornell R, Cote K, Scarcelli JJ, Marshall JK, Tzvetkova B, Figueroa B, DiNino D, Sievers A, Lee C, Guo J, Mahan E, Francis C, Lam K, D'Antona AM, Zollner R, Zhu HL, Kriz R, Somers W, Lin L (2019) Transient CHO expression platform for robust antibody production and its enhanced N-glycan sialylation on therapeutic glycoproteins. Biotechnol Prog 35(1):e2724. https:\/\/doi.org\/10.1002\/btpr.2724","journal-title":"Biotechnol Prog"}],"container-title":["AMB Express"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13568-019-0856-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13568-019-0856-8\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13568-019-0856-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T18:53:45Z","timestamp":1721674425000},"score":1,"resource":{"primary":{"URL":"https:\/\/amb-express.springeropen.com\/articles\/10.1186\/s13568-019-0856-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,29]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["856"],"URL":"https:\/\/doi.org\/10.1186\/s13568-019-0856-8","relation":{},"ISSN":["2191-0855"],"issn-type":[{"type":"electronic","value":"2191-0855"}],"subject":[],"published":{"date-parts":[[2019,8,29]]},"assertion":[{"value":"18 June 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 August 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This article does not contain any studies with human participants or animals performed by any of the authors.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"135"}}