{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:01:26Z","timestamp":1788188486366,"version":"build-2803163510"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["AMB Expr"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Cordyceps cicadae<\/jats:italic> is a well-known traditional Chinese medicine for treating palpitations and eye diseases. It contains several bioactive compounds such as adenosine, N6-(2-hydroxyethyl)-adenosine (HEA), and polysaccharide. Those bioactive compounds have been reported to perform anti-oxidation and anti-inflammatory properties and provide renal protection. In this study, we researched different fermentation conditions in order to enhance the biomass, adenosine, HEA, and polysaccharide productions of <jats:italic>C. cicadae<\/jats:italic> NTTU 868. Solid fermentation was carried out with different grain substrates (barley, oat, rice and wheat). Various submerged fermentation scales were used to produce the <jats:italic>C. cicadae<\/jats:italic> NTTU 868 mycelium. The results of solid fermentation revealed that <jats:italic>C. cicadae<\/jats:italic> NTTU 868 produced higher adenosine and HEA concentrations in oat rather than in other substrates. <jats:italic>C. cicadae<\/jats:italic> NTTU 868 mycelium had obtained the highest concentrations of adenosine and HEA on Day 2 as using the small-scale submerged fermentation. Furthermore, potato dextrose broth with extra 0.2% of yeast extract was able to result in higher HEA concentration. In conclusion, using submerged fermentation to culture <jats:italic>C. cicadae<\/jats:italic> NTTU 868 resulted in more efficient adenosine, HEA, and polysaccharide productions than using solid-fermentation, especially when 0.2% of yeast extract was used in the PDB. Importantly, this can be easily scaled-up in the fermentation industry.<\/jats:p>","DOI":"10.1186\/s13568-019-0892-4","type":"journal-article","created":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T13:03:01Z","timestamp":1575896581000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Using submerged fermentation to fast increase N6-(2-hydroxyethyl)-adenosine, adenosine and polysaccharide productions of Cordyceps cicadae NTTU 868"],"prefix":"10.1186","volume":"9","author":[{"given":"Bo-Jun","family":"Ke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Lin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,12,9]]},"reference":[{"issue":"3","key":"892_CR1","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1111\/j.1365-2672.2005.02640.x","volume":"99","author":"CH Dong","year":"2005","unstructured":"Dong CH, Yao YJ (2005) Nutritional requirements of mycelial growth of Cordyceps sinensis in submerged culture. 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