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Because of the wide application of macrolides in clinical treatment, macrolide-resistant\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    strains have become increasingly common worldwide. However, the molecular mechanisms underlying drug resistance in\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    are poorly understood. In the present work, we analyzed the whole proteomes of macrolide-sensitive and macrolide-resistant strains of\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    using a tandem mass tag-labeling quantitative proteomic technique, Data are available via ProteomeXchange with identifier\u00a0PXD022220. In total, 165 differentially expressed proteins were identified, of which 80 were upregulated and 85 were downregulated in the drug-resistant strain compared with the sensitive strain. Functional analysis revealed that these proteins were predominantly involved in protein and peptide biosynthesis processes, the ribosome, and transmembrane transporter activity, which implicates them in the mechanism(s) of resistance of\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    to macrolides. Our results provide new insights into drug resistance in\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    and identify potential targets for further studies on resistance mechanisms in this bacterium.\n                  <\/jats:p>","DOI":"10.1186\/s13568-021-01187-8","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T15:40:35Z","timestamp":1613144435000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants"],"prefix":"10.1186","volume":"11","author":[{"given":"Shaoli","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guanhua","family":"Xue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanqing","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghua","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghui","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,2,12]]},"reference":[{"key":"1187_CR1","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1128\/aac.11.4.661","volume":"11","author":"NE Allen","year":"1977","unstructured":"Allen NE (1977) Macrolide resistance in Staphylococcus aureus: induction of macrolide-resistant protein synthesis. 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The patient data were anonymously reported. Based on the guidelines of the Ethics Committee of the Capital Institute of Pediatrics, informed consent was not sought from the patients.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that there are no competing interests associated with this manuscript.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"26"}}